Differential Expression of AtXTH17, AtXTH18, AtXTH19 and AtXTH20 Genes in Arabidopsis Roots. Physiological Roles in Specification in Cell Wall Construction

Vissenberg, Kris; Oyama, Mika; Osato, Y.; Yokoyama, Ryusuke; Verbelen, Jean‐Pierre; Nishitani, Kazuhiko

doi:10.1093/pcp/pci013

Cited by 119 publications

(142 citation statements)

References 42 publications

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“…Eight of these genes are differentially regulated in ise1 and/or ise2 compared with WT (Table 2). XTH19 has been reported as root-specific (36,37), yet it is strongly induced in both mutants. Indeed, XTH19 is the most strongly induced gene in ise1 (50-fold higher than WT levels), and it is also strongly induced in ise2 (15-fold WT levels).…”

Section: Ise2 Is Found In Chloroplastsmentioning

confidence: 95%

Organelle–nucleus cross-talk regulates plant intercellular communication via plasmodesmata

Burch‐Smith¹,

Brunkard²,

Choi

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

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We use Arabidopsis thaliana embryogenesis as a model system for studying intercellular transport via plasmodesmata (PD). A forward genetic screen for altered PD transport identified increased size exclusion limit (ise) 1 and ise2 mutants with increased intercellular transport of fluorescent 10-kDa tracers. Both ise1 and ise2 exhibit increased formation of twinned and branched PD. ISE1 encodes a mitochondrial DEAD-box RNA helicase, whereas ISE2 encodes a DEVH-type RNA helicase. Here, we show that ISE2 foci are localized to the chloroplast stroma. Surprisingly, plastid development is defective in both ise1 and ise2 mutant embryos. In an effort to understand how RNA helicases that localize to different organelles have similar impacts on plastid and PD development/function, we performed whole-genome expression analyses. The most significantly affected class of transcripts in both mutants encode products that target to and enable plastid function. These results reinforce the importance of plastid-mitochondria-nucleus cross-talk, add PD as a critical player in the plant cell communication network, and thereby illuminate a previously undescribed signaling pathway dubbed organelle-nucleus-plasmodesmata signaling. Several genes with roles in cell wall synthesis and modification are also differentially expressed in both mutants, providing new targets for investigating PD development and function.

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Section: Ise2 Is Found In Chloroplastsmentioning

confidence: 95%

Organelle–nucleus cross-talk regulates plant intercellular communication via plasmodesmata

Burch‐Smith¹,

Brunkard²,

Choi

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

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“…For example, XTHs are involved in cell elongation by hydrolyzing the cell wall component, xyloglucan, and subsequent loosening of the cell wall (Rose et al, 2002). Among the seven XTH genes that were suppressed in the axr3-1/iaa17-1 mutant, At-XTH17 is expressed in the elongation and differentiating regions of the roots, and At-XTH20 is specifically expressed in the basal mature region of the roots (Vissenberg et al, 2005). Repression of these two XTH genes may be responsible for root growth inhibition in the axr3-1/iaa17-1 mutant.…”

Section: The Axr3/iaa17-3 Mutation and Its Effects On Global Gene Expmentioning

confidence: 99%

Functional Genomic Analysis of theAUXIN/INDOLE-3-ACETIC ACIDGene Family Members inArabidopsis thaliana [W]

et al. 2005

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Auxin regulates various aspects of plant growth and development. The AUXIN/INDOLE-3-ACETIC ACID (Aux/IAA) genes encode short-lived transcriptional repressors that are targeted by the TRANSPORT INHIBITOR RESPONSE1/AUXIN RECEPTOR F-BOX proteins. The Aux/IAA proteins regulate auxin-mediated gene expression by interacting with members of the AUXIN RESPONSE FACTOR protein family. Aux/IAA function is poorly understood; herein, we report the identification and characterization of insertion mutants in 12 of the 29 Aux/IAA family members. The mutants show no visible developmental defects compared with the wild type. Double or triple mutants of closely related Aux/IAA genes, such as iaa8-1 iaa9-1 or iaa5-1 iaa6-1 iaa19-1, also exhibit wild-type phenotypes. Global gene expression analysis reveals that the molecular phenotypes of auxin-treated and untreated light-grown seedlings are unaffected in the iaa17-6 and iaa5-1 iaa6-1 iaa19-1 mutants. By contrast, similar analysis with the gain-of-function axr3-1/iaa17-1 mutant seedlings reveals dramatic changes in basal and auxin-induced gene expression compared with the wild type. Expression of several type-A ARABIDOPSIS RESPONSE REGULATOR genes and a number of genes involved in cell wall biosynthesis and degradation is repressed in axr3-1/iaa17-1. The data suggest extensive functional redundancy among Aux/IAA gene family members and that enhanced stability of the AXR3/IAA17 protein severely alters the molecular phenotype, resulting in developmental defects.

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“…Nevertheless, cesa5 mutants have a seed mucilage phenotype (Sullivan et al, 2011), and vegetative plants are indistinguishable from the wild type (Desprez et al, 2007). XTH17 is expressed mostly in roots (Vissenberg et al, 2005;Becnel et al, 2006). Furthermore, our FT-IR and monosaccharide analyses do not support this hypothesis of KAK involvement in cell wall processes, because the results did not show any major differences in cell wall composition or between esk1, beem396b, esk1 kak-7, and esk1 kak-8.…”

Section: Kak May Be Involved In Vascular Developmentmentioning

confidence: 62%

Suppression of Dwarf and irregular xylem Phenotypes Generates Low-Acetylated Biomass Lines in Arabidopsis

et al. 2015

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(S.H.).eskimo1-5 (esk1-5) is a dwarf Arabidopsis (Arabidopsis thaliana) mutant that has a constitutive drought syndrome and collapsed xylem vessels, along with low acetylation levels in xylan and mannan. ESK1 has xylan O-acetyltransferase activity in vitro. We used a suppressor strategy on esk1-5 to screen for variants with wild-type growth and low acetylation levels, a favorable combination for ethanol production. We found a recessive mutation in the KAKTUS (KAK) gene that suppressed dwarfism and the collapsed xylem character, the cause of decreased hydraulic conductivity in the esk1-5 mutant. Backcrosses between esk1-5 and two independent knockout kak mutants confirmed suppression of the esk1-5 effect. kak single mutants showed larger stem diameters than the wild type. The KAK promoter fused with a reporter gene showed activity in the vascular cambium, phloem, and primary xylem in the stem and hypocotyl. However, suppression of the collapsed xylem phenotype in esk1 kak double mutants was not associated with the recovery of cell wall O-acetylation or any major cell wall modifications. Therefore, our results indicate that, in addition to its described activity as a repressor of endoreduplication, KAK may play a role in vascular development. Furthermore, orthologous esk1 kak double mutants may hold promise for ethanol production in crop plants.

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Differential Expression of AtXTH17, AtXTH18, AtXTH19 and AtXTH20 Genes in Arabidopsis Roots. Physiological Roles in Specification in Cell Wall Construction

Cited by 119 publications

References 42 publications

Organelle–nucleus cross-talk regulates plant intercellular communication via plasmodesmata

Organelle–nucleus cross-talk regulates plant intercellular communication via plasmodesmata

Functional Genomic Analysis of theAUXIN/INDOLE-3-ACETIC ACIDGene Family Members inArabidopsis thaliana [W]

Suppression of Dwarf and irregular xylem Phenotypes Generates Low-Acetylated Biomass Lines in Arabidopsis

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