Differential expression of cytochrome oxidase and ALY-family genes in resistant and susceptible tomato cultivars (Solanum lycopersicum) inoculated with Tomato bushy stunt virus

Hafez, Elsayed E.; Moustafa, Mahmoud

doi:10.1016/j.jgeb.2011.05.010

Cited by 2 publications

(2 citation statements)

References 36 publications

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“…Recently, up-regulation of COX from tomato infected with tomato bushy top virus was reported. The differential expression of the transcript was demonstrated where in higher levels of expression was observed in resistant cultivars in comparison to the susceptible cultivar (HAFEZ and MOUSTAFA, 2011). Increased level of expression of CeCox in casuarina may be indicative of a basic tolerant nature of the species to pathogenic microbes.…”

Section: Distinct Defense -Related Responses Observed Following Elicimentioning

confidence: 95%

Characterization of genes expressed in Casuarina equisetifolia in response to elicitation by cell wall components of Trichosporium vesiculosum

2013

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Casuarina equisetifolia has the widest distribution of all Casuarina species and is a nitrogen-fixing tree planted in tropical/subtropical littoral zones of Asia, the Pacific and Africa for coastal reclamation, charcoal making, pulp and timber. Trichosporium vesiculosum, the causal agent of blister bark disease, is a serious pathogen of C. equisetifolia. The present study was undertaken to understand the molecular mechanisms involved during pathogen cell wall elicitation in this hardy tree species. Transcript profiling during elicitation induced by cell wall components of T. vesiculosum revealed expression of resistance genes; cytochrome oxidase; trans membrane proteins; genes involved in programmed cell death like 26S proteasome and ubiquitin activating enzyme; early nodulin gene, wound inducible metallocarboxy peptidase inhibitor, glucanase, metal binding protein and signal recognition particle. The fold expression of selected transcripts including glucanase, 26 S proteasome, signal recognition particle, cytochrome oxidase and the metal binding protein using RT-qPCR revealed 12-59 fold increase in expression after 48 hours of elicitor treatment. The expression of these transcripts during abiotic stresses like heat, mechanical wounding, salt (NaCl) and drought (PEG) was also analyzed. Glucanase was up-regulated significantly during wounding and heat stress while proteasome was up-regulated 1-4 fold during NaCl, PEG and wounding stress. The novel transcript CeHMA was up-regulated under all the stress conditions. This is the first report on molecular defense in C. equisetifolia and has provided a pool of candidate genes for detailed molecular dissection to further broaden the knowledge on the response of woody perennials during pathogen cell wall elicitation.

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Section: Distinct Defense -Related Responses Observed Following Elicimentioning

confidence: 95%

Characterization of genes expressed in Casuarina equisetifolia in response to elicitation by cell wall components of Trichosporium vesiculosum

2013

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“…Pathogen induced plant Glycotransferase-like proteins have been shown to be associated with defence responses in plant [97]. Cytochrome oxidase genes were upregulated in disease resistant varieties in comparision to susceptible tomato varieties [98]. Moreover, cytochrome oxidase (chlorophyll) related genes were found to be downregulated and targeted by Zymoseptoria during infection of wheat [57].…”

Section: Studies Of Microbiome Mediated Suppression Of Soilborne Dise...mentioning

confidence: 99%

Multi omics aided small RNA profiling of wheat rhizosphere and their potential targets in contrasting soils forRhizoctonia solani-AG8 suppression

Regmi,

Panchal,

Hicks

et al. 2024

Preprint

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Next-generation sequencing helps describe microbial communities in rhizosphere environments, but understanding rhizosphere-plant interactions’ synergistic effects on plant traits and health outcomes remains challenging. This study analyses rhizosphere sRNAs’ ability to manipulate host gene targets in plants grown in suppressive (SP) and non-suppressive (NSP) soils with an integrated multi omics dataset. The results showed that rhizosphere sRNAs exhibited specific compositional features that may be important for rhizosphere-plant interaction. Small RNAs, less than 30 nt in size, were predominant in both samples, with a 5-prime bias towards cytosine enrichment, suggesting potential association with wheat specific argonauts. Mapping of sRNA reads to microbial metagenomes assembled draft genomes from SP and NSP soils showed sRNA loci were differentially expressed (DE) between the soils with contrasting disease suppressive capacities. In total, 96 and 132 non redundant rhizosphere sRNAs were abundant in SP and NSP rhizosphere communities, respectively. While 55 known bacterial sRNA loci were predicted from both SP and NSP metagenomes, 127 sRNAs originated from these loci were differentially expressed. Global wheat target prediction and functional analysis from DE rhizosphere sRNAs showed both soil type specific and common pathways. Upregulated NSP sRNAs target metabolic pathways, secondary metabolite biosynthesis, MAPK signalling, while SP sRNAs target glycerophospholipid metabolism, pathways such as polycomb repressive complex, starch/sucrose metabolism, and plant-pathogen interactions were targeted by both sets of sRNAs. This is the first study showing evidence for rhizosphere sRNAs and their corresponding plant transcripts in the context of biological disease suppression in agricultural soils.ImportanceSmall RNAs (sRNAs) have gained attention in host-microbe interactions due to their diverse roles in controlling biological processes. Studies have identified numerous sRNAs with novel functions across various organisms. Echoing growing evidence of sRNAs in different plant-microbe interaction, we show an evidence of rhizosphere sRNAs regulating wheat genes in soil disease suppression context. This understanding could significantly enhance our comprehension of gene regulation in biological functions, potentially paving the way for the development of microbiome-based methods to influence host traits. Understanding the microbiome community’s mechanisms in different environments offers opportunities to modify them for agriculture, including modifying farming practices, host genetics/immunity, and synthetic communities for disease suppression.

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Differential expression of cytochrome oxidase and ALY-family genes in resistant and susceptible tomato cultivars (Solanum lycopersicum) inoculated with Tomato bushy stunt virus

Cited by 2 publications

References 36 publications

Characterization of genes expressed in Casuarina equisetifolia in response to elicitation by cell wall components of Trichosporium vesiculosum

Characterization of genes expressed in Casuarina equisetifolia in response to elicitation by cell wall components of Trichosporium vesiculosum

Multi omics aided small RNA profiling of wheat rhizosphere and their potential targets in contrasting soils forRhizoctonia solani-AG8 suppression

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