Differential Expression of EC‐SOD, Mn‐SOD and CuZn‐SOD in Rat Lung Exposed to Crystalline Silica

Kim, Heungnam; Morimoto, Yasuo; Ogami, Akira; Nagatomo, Hiroko; Hirohashi, Masami; Oyabu, Takako; Kawanami, Yukiko; Kuroda, Etsushi; Higashi, Toshiaki; Tanaka, Isamu

doi:10.1539/joh.49.242

Cited by 24 publications

(27 citation statements)

References 25 publications

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“…Our previous study using crystalline silica showed that even if the particles are of the same chemical composition, their biological effects vary depending on their particle size 4) . Hazard assessment of newly-generated respirable particles must therefore be conducted for such differences and various biomarker studies are currently being implemented to objectively and quantitatively assess the hazards of such substances [5][6][7][8][9][10][11][12][13][14] .…”

Section: Background and Objectivesmentioning

confidence: 99%

Analysis of Bronchoalveolar Lavage Fluid Adhering to Lung Surfactant

Kadoya¹,

Ogami²,

Morimoto³

et al. 2012

Ind Health

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Nickel oxide with two different particle sizes, micron size (NiO) and submicron size (nNiOm), as well as crystalline silica as a positive control and titanium dioxide as a negative control, were intratracheally instilled in rats and the phospholipid concentration and the protein concentration and surface tension of bronchoalveolar lavage fluid (BALF), which are used in surfactant assessment, were measured to see if they could be effective biomarkers in toxicity assessment. The results showed that the NiO instilled group showed no significant difference compared to the control group throughout the observation period. In contrast, a significant difference was found in the nNiOm instilled group compared to the control group throughout the observation period. Moreover, a significant difference was found in the crystalline silica instilled group for each measurement compared to the control group while for the titanium dioxide group, almost no significant difference was found. These results indicate that submicronsized particles of nickel oxide with smaller median diameters potentially have a stronger biological effect than micron size particles. They also indicate that screening can be done by measuring the phospholipid concentration and the protein concentration and surface tension of BALF.

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Section: Background and Objectivesmentioning

confidence: 99%

Analysis of Bronchoalveolar Lavage Fluid Adhering to Lung Surfactant

Kadoya¹,

Ogami²,

Morimoto³

et al. 2012

Ind Health

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“…It catalyzes the dismutation of superoxide radicals into hydrogen peroxide and oxygen (11). EC-SOD is highly expressed in arteries (3,12), lungs, and airways (13,14), with high levels in alveolar macrophages (15). Human (16) and mouse studies (17)(18)(19) have shown that EC-SOD plays a key role in attenuating lung injury by blocking oxidative stressmediated lung inflammation; however, little is known about the role of EC-SOD in modulating allergic airway disease.…”

Section: Introductionmentioning

confidence: 99%

The R213G polymorphism in SOD3 protects against allergic airway inflammation

Gaurav¹,

Varasteh²,

Weaver³

et al. 2017

JCI Insight

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“…In lung disorders caused by dust, phagocytosis of dust induces infiltration of neutrophils and alveolar macrophages, and persistent or progressed inflammation is likely to cause lung injury and lead to irreversible changes, such as fibrosis and tumors (Nishi et al 2009; Borm and Driscoll 1996; Kim et al 2007). Persistent inflammation has been reported in an animal exposure model using asbestos and silica as materials known to have high toxicity (Fubini and Hubbard 2003; Schins 2002).…”

Section: Introductionmentioning

confidence: 99%

Pulmonary toxicity of well-dispersed cerium oxide nanoparticles following intratracheal instillation and inhalation

et al. 2015

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We performed inhalation and intratracheal instillation studies of cerium dioxide (CeO2) nanoparticles in order to investigate their pulmonary toxicity, and observed pulmonary inflammation not only in the acute and but also in the chronic phases. In the intratracheal instillation study, F344 rats were exposed to 0.2 mg or 1 mg of CeO2 nanoparticles. Cell analysis and chemokines in bronchoalveolar lavage fluid (BALF) were analyzed from 3 days to 6 months following the instillation. In the inhalation study, rats were exposed to the maximum concentration of inhaled CeO2 nanoparticles (2, 10 mg/m3, respectively) for 4 weeks (6 h/day, 5 days/week). The same endpoints as in the intratracheal instillation study were examined from 3 days to 3 months after the end of the exposure. The intratracheal instillation of CeO2 nanoparticles caused a persistent increase in the total and neutrophil number in BALF and in the concentration of cytokine-induced neutrophil chemoattractant (CINC)-1, CINC-2, chemokine for neutrophil, and heme oxygenase-1 (HO-1), an oxidative stress marker, in BALF during the observation time. The inhalation of CeO2 nanoparticles also induced a persistent influx of neutrophils and expression of CINC-1, CINC-2, and HO-1 in BALF. Pathological features revealed that inflammatory cells, including macrophages and neutrophils, invaded the alveolar space in both studies. Taken together, the CeO2 nanoparticles induced not only acute but also chronic inflammation in the lung, suggesting that CeO2 nanoparticles have a pulmonary toxicity that can lead to irreversible lesions.

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Differential Expression of EC‐SOD, Mn‐SOD and CuZn‐SOD in Rat Lung Exposed to Crystalline Silica

Cited by 24 publications

References 25 publications

Analysis of Bronchoalveolar Lavage Fluid Adhering to Lung Surfactant

Analysis of Bronchoalveolar Lavage Fluid Adhering to Lung Surfactant

The R213G polymorphism in SOD3 protects against allergic airway inflammation

Pulmonary toxicity of well-dispersed cerium oxide nanoparticles following intratracheal instillation and inhalation

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