Differential expression of EphA5 protein in gastric carcinoma and its clinical significance

Abstract

The aim of the present study was to evaluate ephrin type-A receptor 5 (EphA5) expression and its clinicopathological significance in gastric cancer. Gastric cancer tissues were analyzed by immunohistochemistry. The association between EphA5 expression and clinicopathological parameters, human epidermal growth factor receptor 2 (HER2) status and Ki-67 proliferation index was statistically analyzed. EphA5 expression was detected in all non-tumor gastric epithelia but was differentially expressed among gastric cancer samples. EphA5 was negatively expressed in 30/110 (27.3%) and positively expressed in 80/110 (72.3%) samples from patients with gastric cancer. EphA5 expression was significantly associated with Lauren classification (P= 0.032), lymph node metastasis (P<0.001), HER2 expression (P=0.020) and Ki-67 expression (P=0.005). No significant association was determined between EphA5 expression and age, sex, primary location, depth of invasion and Tumor-Node-Metastasis stage. The present data indicated that EphA5 is differentially expressed in gastric cancer. EphA5 may therefore be a potential therapeutic target and may have clinical utility as a marker for lymph node metastasis in gastric cancer.

EphA5 knockdown enhances the invasion and migration ability of esophageal squamous cell carcinoma via epithelial-mesenchymal transition through activating Wnt/β-catenin pathway

“…However, other previous studies proved that EphA5 was detected to be low-expressed in some other malignant tumors [6][7][8][9][10]. The low expression of EphA5 was associated with lymph node metastasis of breast cancer [10], colorectal cancer [7] and gastric cancer [6], indicating poor prognosis in colorectal carcinoma [7] and ovarian cancer [8]. Contradictory with the above reports, we found that high EphA5 expression implies a greater likelihood of regional lymph node metastasis and advanced tumor stage, while low EphA5 expression may be related to nerve invasion.…”

“…And in high-grade hepatocellular carcinoma,the EphA5 gene was also demonstrated to be upregulated [15,16]. However, other previous studies proved that EphA5 was detected to be low-expressed in some other malignant tumors [6][7][8][9][10]. The low expression of EphA5 was associated with lymph node metastasis of breast cancer [10], colorectal cancer [7] and gastric cancer [6], indicating poor prognosis in colorectal carcinoma [7] and ovarian cancer [8].…”

“…Recent studies have also shown that these receptors regulate cell adhesion, differentiation and stemness [5]. EphA5, a member of Eph receptors, has been implicated in various biological activities, including tumorigenesis and progression of different cancers [6][7][8][9][10], radioresistance in lung cancer [11] and sensitivity to trastuzumab in HER2-positive breast cancer [12]. However, the role of EphA5 in patients with ESCC remains unknown.…”

The Significance of MicroRNAs Expression in Regulation of Extracellular Matrix and Other Drug Resistant Genes in Drug Resistant Ovarian Cancer Cell Lines

“…It was reported that signal transduction by the EPHA7 receptor may activate the extracellular-signal-regulated kinase (ERK) signaling pathway that also involves MAP3K8 [86]. Upregulation of EPHA7 was observed in many cancers, including hepatocellular carcinoma, where it was associated with tumor progression, invasiveness, and metastasis [87]. MAP3K8 was described as a mediator of vemurafenib resistance of thyroid cancer stem cells [88].…”

Multiple paragangliomas: a case report

“…Mutations in these genes have first been found in paragangliomas/pheochromocytomas. TENM3 and EPHA5 encode for proteins with important functions in neuronal cells and have been shown to be involved in tumorigenesis [17][18][19][20].…”