Differential expression of GDF9, TGFB1, TGFB2 and TGFB3 in porcine oocytes isolated from follicles of different size before and after culture in vitro

Jackowska, M.; Kempisty, Bartosz; Woźna, M.; Piotrowska, H.; Antosik, Paweł; Zawierucha, Piotr; Bukowska, Dorota; Nowicki, Michał; Jaśkowski, J. M.; Brüssow, Klaus‐Peter

doi:10.1556/avet.2012.061

Cited by 26 publications

(23 citation statements)

References 25 publications

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“…Our recent study focused on the expression analysis of TGFB genes including TGFB1, TGFB2, TGFB3, and GDF9 in porcine oocytes before and after in vitro maturation (IVM), as well as in gametes collected from follicles of various sizes (16). We found an increased expression of TGFB1 and TGFB2 in oocytes isolated from larger size follicles compared to medium and small ones, whereas TGFB3 and GDF9 expression was unchanged.…”

Section: Resultsmentioning

confidence: 98%

Expression of integrins and GDF9 mRNAs is associated with ovarian follicle size and donor puberty status in pigs

Antosik¹,

Ješeta²,

Kranc³

et al. 2016

Medycyna Weterynaryjna

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The proper development of cumulus-oocyte complexes (COCs) is orchestrated by the normal course of folliculogenesis and oogenesis. It is well accepted that folliculogenesis and oogenesis are associated with many morphological, molecular, and metabolic changes within growing ovarian follicles and COCs (8,11,12,19,20,27,28,30). These modifications involve the secretion of multiple factors, including growth factors and hormones that regulate synchronous nuclear Expression of integrins and GDF9 mRNAs is associated with ovarian follicle size and donor puberty status in pigs 1) Summary Ovarian folliculogenesis and oogenesis has a significant impact on embryo growth and development in preimplantation stages. Although both processes are widely understood in several species of mammals, including pigs, the factors influencing the proper maturation capability of oocytes, as well as the developmental competence of the surrounding somatic granulosa cells (GCs) and cumulus cells (CCs), are still not entirely known. This study aimed to investigate the expression of growth differentiation factor 9 (GDF9) and integrins (ITGB1, ITGB2, ITGB3 and ITGB4) in porcine oocytes isolated from follicles of various size and donors characterized by different puberty status. The relative abundance of GDF9, ITGB1, ITGB2, ITGB3, and ITGB4 mRNAs in porcine oocytes isolated from medium follicles of cycling sows (MFCS), small follicles of juvenile gilts (SFJG), and small follicles of cycling sows (SFCS) was assessed by an RT-qPCR assay. It was found an increased expression of GDF9 in oocytes isolated from the small follicles of juvenile gilts as compared to the other two groups (P < 0.001). A significant down-regulation of ITGB1 and ITGB2 oocyte mRNAs collected from medium follicles of cycling sows was observed (P < 0.05 and P < 0.001, respectively). The ITGB3 mRNA expression was significantly decreased in oocytes isolated from small follicles of juvenile gilts (P < 0.001), whereas a lower expression of ITGB4 in oocytes from both medium follicles (cycling sows) and small follicles (juvenile gilts) was observed. In conclusion, GDF9 may be recognized as the main factor regulating follicle growth at early stages of folliculogenesis. The expression of ITGBs is significantly regulated by the puberty status of donor pigs, and different follicular sizes may play a subordinate role in integrin expression during in vivo follicle development in pigs.

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Section: Resultsmentioning

confidence: 98%

Expression of integrins and GDF9 mRNAs is associated with ovarian follicle size and donor puberty status in pigs

Antosik¹,

Ješeta²,

Kranc³

et al. 2016

Medycyna Weterynaryjna

Self Cite

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“…Genes belonging to TGFB superfamily are important regulators; they are called also “checkpoints of oocyte maturation potential" [23]. INHA and INHB are members of TGFB superfamily and it is suggested that they regulate important stages of oocyte maturation in vivo and in vitro [24–26].…”

Section: Discussionmentioning

confidence: 99%

Expression and Cellular Distribution of INHA and INHB before and afterIn VitroCultivation of Porcine Oocytes Isolated from Follicles of Different Size

Kempisty

Jackowska

Woźna

et al. 2012

Journal of Biomedicine and Biotechnology

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Cumulus-oocyte-complexes (COCs) were collected from small (<3 mm), medium (3–5 mm), and large (>5 mm) porcine follicles, and the INHA and INHB expression and cellular localization were studied. Developmentally competent (BCB+) COCs were cultured for 44 h. Samples of mRNA were isolated before and after in vitro maturation (IVM) from oocytes collected from follicles of different size for RQ-PCR assay. The INHA and INHB protein distribution within the oocytes was observed by confocal microscopy. INHA mRNA expression was increased in oocytes from large compared to medium and small follicles before IVM (P < 0.001), and to oocytes of small follicles after IVM (P < 0.001). The INHB expression was not different before IVM, but the IHNB mRNA level was gradually higher in oocytes from large follicles after IVM (P < 0.01). INHA was not differently expressed before IVM; however, in large follicle oocytes the protein was distributed in the peripheral area of the cytoplasm; in oocytes from small follicles it was in the entire cytoplasm. After IVM, INHA was strongly expressed in oocytes from small follicles and distributed particularly in the zona pellucida (ZP). Similarly and both before and after IVM, INHB protein was highly expressed in small follicle oocytes and within the cytoplasm. In summary, INHs can be recognized as a marker of porcine oocyte quality.

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“…Gene expression studies using mice and rats showed that TGFB3 are involved in reproductive functions, such as gonadal and secondary sex organ [42][43][44][45]. In swine, the TGFB gene superfamily, which includes TGFB3, has been identified to be expressed in ovarian follicles of different sizes before and after in vitro culture in porcine oocytes collected from crossbred Landrace gilts at 155 days of age [46]. This study suggested that the TGFB gene superfamily is associated with the stage of maturation of porcine oocytes and the follicle size [46].…”

Section: Genome-wide Association Analysismentioning

confidence: 99%

“…In swine, the TGFB gene superfamily, which includes TGFB3, has been identified to be expressed in ovarian follicles of different sizes before and after in vitro culture in porcine oocytes collected from crossbred Landrace gilts at 155 days of age [46]. This study suggested that the TGFB gene superfamily is associated with the stage of maturation of porcine oocytes and the follicle size [46]. With regards to IRF2BPL, this gene encodes for a transcription factor that regulates neuronal networks controlling female reproductive function in nonhuman primates and rodents [47].…”

Section: Genome-wide Association Analysismentioning

confidence: 99%

Genetic and genomic characterization of vulva size traits in Yorkshire and Landrace gilts

Corredor

Sanglard

Leach³

et al. 2020

BMC Genet

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Background: Reproductive performance is critical for efficient swine production. Recent results indicated that vulva size (VS) may be predictive of reproductive performance in sows. Study objectives were to estimate genetic parameters, identify genomic regions associated, and estimate genomic prediction accuracies (GPA) for VS traits. Results: Heritability estimates of VS traits, vulva area (VA), height (VH), and width (VW) measurements, were moderately to highly heritable in Yorkshire, with 0.46 ± 0.10, 0.55 ± 0.10, 0.31 ± 0.09, respectively, whereas these estimates were low to moderate in Landrace, with 0.16 ± 0.09, 0.24 ± 0.11, and 0.08 ± 0.06, respectively. Genetic correlations within VS traits were very high for both breeds, with the lowest of 0.67 ± 0.29 for VH and VW for Landrace. Genome-wide association studies (GWAS) for Landrace, reveled genomic region associated with VS traits on Sus scrofa chromosome (SSC) 2 (154-157 Mb), 7 (107-110 Mb), 8 (4-6 Mb), and 10 (8-19 Mb). For Yorkshire, genomic regions on SSC 1 (87-91 and 282-287 Mb) and 5 (67 Mb) were identified. All regions explained at least 3.4% of the genetic variance. Accuracies of genomic prediction were moderate in Landrace, ranging from 0.30 (VH) to 0.61 (VA), and lower for Yorkshire, with 0.07 (VW) to 0.11 (VH). Between-breed and multi-breed genomic prediction accuracies were low. Conclusions: Our findings suggest that VS traits are heritable in Landrace and Yorkshire gilts. Genomic analyses show that major QTL control these traits, and they differ between breed. Genomic information can be used to increase genetic gains for these traits in gilts. Additional research must be done to validate the GWAS and genomic prediction results reported in our study.

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Differential expression of GDF9, TGFB1, TGFB2 and TGFB3 in porcine oocytes isolated from follicles of different size before and after culture in vitro

Cited by 26 publications

References 25 publications

Expression of integrins and GDF9 mRNAs is associated with ovarian follicle size and donor puberty status in pigs

Expression of integrins and GDF9 mRNAs is associated with ovarian follicle size and donor puberty status in pigs

Expression and Cellular Distribution of INHA and INHB before and afterIn VitroCultivation of Porcine Oocytes Isolated from Follicles of Different Size

Genetic and genomic characterization of vulva size traits in Yorkshire and Landrace gilts

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