Differential expression of genes related to gain and intake in the liver of beef cattle

Zarek, C. M.; Lindholm‐Perry, A. K.; Kuehn, L. A.; Freetly, H. C.

doi:10.1186/s13104-016-2345-3

Cited by 204 publications

(247 citation statements)

References 21 publications

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“…Although the RFI values pertaining to the animals used in this study showed clear signi cant divergence between high and low-RFI groups in both heifers and bulls, we failed to identify any DEGs within the liver tissue of the heifers and the skeletal muscle tissue of the bulls. Similarly, other studies evaluating the molecular control of RFI divergence in crossbred steers reported no DEGs following correction for multiple testing [29][30][31]. Therefore the main observation of the current study is the inconsistent effect of RFI across (i) gender and (ii) tissue for DEG pro les despite a 10% and 15% difference in DMI between high and low RFI heifers and bulls, respectively, with no difference in ADG in the current study.…”

Section: Discussionsupporting

confidence: 62%

Effect of Gender and Residual Feed Intake Phenotype on the Transcriptome of Liver and Skeletal Muscle Tissue in Purebred Simmental Beef Cattle

McKenna

Keogh

Porter

et al. 2020

Preprint

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Background: The selection of cattle with enhanced feed efficiency is of paramount importance with regard to reducing feed costs in the beef industry. However the role, if any, of gender to the underlying molecular control of feed efficiency in cattle is not currently known. Global transcriptome profiling was undertaken on liver and skeletal muscle biopsies from Simmental heifers and bulls divergent in residual feed intake (RFI) feed efficiency phenotype, in order to identify differentially expressed genes that may be associated with this trait. Results: We identified 5 genes (p<0.001; false discovery rate (FDR) <0.1) to be differentially expressed in skeletal muscle between high and low RFI heifers with all 5 transcripts being up-regulated in the low RFI phenotype. Among these differentially expressed genes, all transcripts were involved in oxidative phosphorylation and mitochondrial homeostasis. A total of 11 genes (p<0.001;FDR <0.1) were differentially expressed in hepatic tissue between high and low RFI bulls with 8 transcripts being up-regulated and 3 being down-regulated in the low RFI phenotype. These differentially expressed genes were related to oxidative response, protein mediation and cell signalling. No genes were identified as differentially expressed in both heifer liver and bull muscle analyses. Conclusions: Results from this study show a clear effect of gender to the underlying molecular control of RFI in cattle, which may be attributable to differences in the physiological age between heifers and bulls. Despite this we have highlighted a number of genes that may hold potential as molecular biomarkers for RFI cattle.

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Section: Discussionsupporting

confidence: 62%

Effect of Gender and Residual Feed Intake Phenotype on the Transcriptome of Liver and Skeletal Muscle Tissue in Purebred Simmental Beef Cattle

McKenna

Keogh

Porter

et al. 2020

Preprint

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“…Amongst the differences identified were the poor knowledge on bTB transmission to people, consumption of uninspected meat and the habit of drinking raw milk during milking, implying that the herdsmen are more at risk of M. bovis infection. Several investigations have reported the potential risk of bTB transmission through drinking of raw milk and contact with infected cattle [48][49][50]. Poor practices regarding TB have been reported amongst high risk groups such as the herdsmen and M. bovis transmission to people was confirmed in a study of livestock workers in Nigeria [35,51].…”

Section: Discussionmentioning

confidence: 97%

Risk practices for bovine tuberculosis transmission to cattle and livestock farming communities living at wildlife-livestock-human interface in northern KwaZulu Natal, South Africa

et al. 2020

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Bovine tuberculosis (bTB) is a disease of cattle that is transmitted through direct contact with an infected animal or ingestion of contaminated food or water. This study seeks to explore the local knowledge on bTB, obtain information on social and cultural practices regarding risk of bTB transmission to cattle and humans (zoonotic TB) in a traditional livestock farming community with a history of bTB diagnosis in cattle and wildlife. Information was collected using a qualitative approach of Focus Group Discussions (FGDs) targeting household members of livestock farmers that owned bTB tested herds. We conducted fourteen FGDs (150 individuals) across four dip tanks that included the following categories of participants from cattle owning households: head of households, herdsmen, dip tank committee members and women. The qualitative data was managed using NVivo Version 12 Pro software. Social and cultural practices were identified as major risky practices for bTB transmission to people, such as the consumption of undercooked meat, consumption of soured /raw milk and lack of protective measures during slaughtering of cattle. The acceptance of animals into a herd without bTB pre-movement testing following traditional practices (e.g. lobola, 'bride price', the temporary introduction of a bull for 'breeding'), the sharing of grazing and watering points amongst the herds and with wildlife were identified as risky practices for M. bovis infection transmission to cattle. Overall, knowledge of bTB in cattle and modes of transmission to people and livestock was found to be high. However, the community was still involved in risky practices that expose people and cattle to bovine TB. An inter-disciplinary 'One Health' approach that engages the community is recommended, to provide locally relevant interventions that allows the community to keep their traditional PLOS NEGLECTED TROPICAL DISEASES

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“…It is possible that heterogeneity among the Leishmania parasites in different endemic regions may cause differences in the immune responses of infected hosts [53]. Therefore, despite the good performance of rK39-ICT, the limitations of this test are low sensitivity and poor specificity in some of the regions endemic for VL [51,54]. Bhattacharyya et al showed that genetic diversity, and a polymorphism in the sequence of rK39 occurring in East Africa and South Asia, influence the performance of rK39-based assay [55].…”

Section: Elisa Testmentioning

confidence: 99%

Leishmania infantum recombinant kinesin degenerated derived repeat (rKDDR): A novel potential antigen for serodiagnosis of visceral leishmaniasis

et al. 2019

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Visceral leishmaniasis (VL) or kala-azar, the most severe form of leishmaniasis, can lead to death if not properly diagnosed and treated. Correct identification of infected patients and reservoirs is vital for controlling the spread of leishmaniasis. Current diagnostic kits for leishmaniasis show high sensitivity and specificity, but can also result in false negatives and cross reactions with related parasitic infections. New diagnostic methods with greater accuracy are urgently needed for diagnosis of leishmaniasis. In this study, we aimed to uncover a new highly effective antigen for the diagnosis of visceral leishmaniasis in dogs and humans, aiming to improve the accuracy compared with those of current methods of diagnosis. Initially, in-silico epitope prediction analyses identified several potential B-cell epitopes in the repetitive region of Leishmania infantum kinesin, which co-localized with predicted structural disordered regions, suggesting high potential for antigenicity. Based on this analysis, 8.5 genomic motifs, which encode the repetitive sequence of 39 degenerate amino acids, were selected for recombinant expression. BLASTn analysis of this repetitive region indicated that it is absent in the T. cruzi parasite, which is closely related to Leishmania, indicating the specificity of this region. This potentially antigenic protein, named recombinant kinesin degenerated derived repeat (rKDDR), was recombinantly expressed in Escherichia coli BL21-Star using the pET28a-TEV expression vector. We then evaluated the performance of rKDDR in correctly diagnosing Leishmania infection and compared this new assay with currently used diagnostic tests for leishmaniasis. rKDDR showed greater sensitivity and specificity in correctly diagnosing leishmaniasis both in human (sensitivity 92.86% and specificity 100%) and canine (sensitivity 88.54% and specificity 97.30%) sera compared with those of rK39 (human: sensitivity 90.48% and specificity 97.92%; canine: sensitivity 78.13% and specificity 90.09%). In addition, the rKDDR-ELISA outperformed the EIE-LVC

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Differential expression of genes related to gain and intake in the liver of beef cattle

Cited by 204 publications

References 21 publications

Effect of Gender and Residual Feed Intake Phenotype on the Transcriptome of Liver and Skeletal Muscle Tissue in Purebred Simmental Beef Cattle

Effect of Gender and Residual Feed Intake Phenotype on the Transcriptome of Liver and Skeletal Muscle Tissue in Purebred Simmental Beef Cattle

Risk practices for bovine tuberculosis transmission to cattle and livestock farming communities living at wildlife-livestock-human interface in northern KwaZulu Natal, South Africa

Leishmania infantum recombinant kinesin degenerated derived repeat (rKDDR): A novel potential antigen for serodiagnosis of visceral leishmaniasis

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