Differential expression of growth and immunity related genes influenced by in ovo supplementation of amino acids in broiler chickens

Bhanja, S.K.; Sudhagar, M; Goel, Akshat; Pandey, Nitin; Mehra, Manish; Agarwal, Sushil; Mandal, Asit Baran

doi:10.17221/7651-cjas

Cited by 43 publications

(48 citation statements)

References 45 publications

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“…() showed the enhanced FI of in ovo Thr‐administered group during days 14–21 of age. Furthermore, Arg, Thr and methionine + cysteine in ovo inoculation was found to increase the mucin gene expression in broiler chicks which may contribute to GIT development and subsequently higher FI (Kadam et al., ; Bhanja et al., ). Saki et al.…”

Section: Discussionmentioning

confidence: 99%

Effect of arginine and threonine administered in ovo on digestive organ developments and subsequent growth performance of broiler chickens

Tahmasebi

Toghyani

2015

Animal Physiology Nutrition

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This trial was conducted to investigate the effect of arginine (Arg), threonine (Thr) and Arg + Thr administered in ovo on growth performance, digestive organs and intestinal morphology in broiler chickens. On day 14 of incubation, 400 fertile eggs were randomly allotted to five experimental treatments through injection in the amniotic fluid including: (i) control (none injected), (ii) sham (0.5 ml of 0.5% saline), (iii) Arg (35 mg/egg), (iv) Thr (25 mg/egg) and (v) Arg + Thr (35 + 25 mg/egg). After hatching, chicks were given a commercial corn-soya bean diet up to 42 days of age. Daily feed intake (FI) and body weight (BW) of chicks were measured during different periods of the trial. Digestive organs were measured for their relative weight and intestinal length on days 11 and 42 of age. Intestinal morphometric traits were evaluated on day 11 of the experiment. Supplementing amino acids affected the performance of broiler chicks as Thr significantly increased FI and BW across starter, grower and finisher periods compared with sham and control (p < 0.05). Furthermore, Arg + Thr injection increased jejunal weight compared with control on day 42 (p < 0.05). Moreover, Arg inclusion led to the greatest villus height and crypt depth among treatments in duodenum (p > 0.05); however, amino acid supplemented groups had lower villus height than control in jejunum (p < 0.05). Negative correlations found between digestive organs related to day 11 of age containing pancreas (r = -0.484; p = 0.030), duodenal (r = -0.577; p = 0.007) as well as ileal lengths (r = -0.471; p = 0.035) and FI of entire period. Otherwise, positive relationships were observed between duodenum (r = 0.580; p = 0.007) and ileum (r = 0.582; p = 0.007) weights on day 42 and FI of chickens across the entire phase. In conclusion, Arg and particularly Thr injection into amnion can improve FI and post-hatch growth performance of chickens which may be mediated by the development of digestive organs.

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Section: Discussionmentioning

confidence: 99%

Effect of arginine and threonine administered in ovo on digestive organ developments and subsequent growth performance of broiler chickens

Tahmasebi

Toghyani

2015

Animal Physiology Nutrition

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“…The total RNA isolation from tissue and cDNA synthesis was carried out as per the procedure of Bhanja et al (2014). The Oligonucleotide sequences of the primers used for the gene expression study are presented in Table 2.…”

Section: Methodsmentioning

confidence: 99%

Effect of mannan-oligosaccharides (MOS) supplementation on performance, immunity and HSP70 gene expression in broiler chicken during hot-dry summer

Rokade

Kagate

Bhanja

et al. 2017

IJAR

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An experiment was conducted on broiler chicks (n=120), reared in cages on a standard diet up to 14 th day of age and thereafter up to 42 nd day (14-42d) on test diets with or without MOS. The 14 th day old chicks were randomly distributed into three dietary treatment groups viz., T 1 (control diet), T 2 (control diet with MOS @ 0.3%) and T 3 (control diet with MOS @ 0.5%). Each dietary treatment comprised of four replicates of eight birds each. Experiment was carried out during hot-dry summer. It was found that production indices improved (P<0.001) significantly during all phases. Cellular and humoral immunity at 28 th day of age improved significantly (P<0.05) due to MOS supplementation. The percentage of hemoglobin, protein, AST and ALT increased significantly (P<0.001) in MOS supplemented group at 28 th as well as 42 nd day of age. While H:L ratio, serum corticosterone and cholesterol levels decreased significantly (P<0.001) in MOS supplemented groups. Supplementation of MOS at the both levels caused significant (P<0.001) down regulation of HSP70 expression in jejunum tissues during 28 th and 42 nd day. Based on this study it was concluded that supplementation of 0.3% MOS in broiler diets significantly improved their performance and welfare during heat stressed conditions.

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“…The weight of bursa, spleen and thymus were recorded and expressed as per cent of live weight. Approximately 150 mg of spleen tissue was collected from each bird and total RNA isolation and cDNA synthesis were carried out as per the procedure of Bhanja et al (2014). The oligonucleotide sequences of the primers used for the gene expression study are presented in Table 1.…”

Section: Immune Organ Development Tissue Collection and Immune Gene mentioning

confidence: 99%

“…The oligonucleotide sequences of the primers used for the gene expression study are presented in Table 1. The expression of immune-related gene encoding IL-6 (interleukin 6), TLR-2 (toll-like receptor-2) and TNF-α (tumour necrosis factor) was analysed by real-time PCR, using an iQ5 cycler following a previously described procedure (Bhanja et al 2014).…”

Section: Immune Organ Development Tissue Collection and Immune Gene mentioning

confidence: 99%

Delayed post-hatch feeding affects the performance and immunocompetence differently in male and female broiler chickens

Tamboli

Goel

Mehra

et al. 2017

Journal of Applied Animal Research

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The effect of post-hatch (PH) feed deprival for 6, 12, 24 and 36 h was studied in male and female broiler chickens. At 21 d, lower body weight (BW) was recorded in 36 h feed-deprived (FD) birds; however, at 42 d PH, only 36 h FD female birds had lower BW compared to control and other FD birds. Feed intake during 0-21 d PH was lower in 36 h FD birds, but feed conversion ratio did not differ between control and FD birds. The H:L ratio significantly increased in 12-36 h FD male birds and 24-36 h FD females. The humoral immune response was similar in FD and control birds, but the cellular immune response was higher in 12 and 24 h FD female birds. At 36 h the expression of interleukin 6 (IL-6), toll-like receptor-2 (TLR-2) and tumour necrosis factor-α-gene was down-regulated in male birds only. However, the expression of IL-6 and TLR-2 was up-regulated in 12-36 h FD female birds at 7 and 14 d PH. It may be concluded that PH feed deprival for the first 24 h did not affect growth performance but improved immune response in slow-growing broiler females. ARTICLE HISTORY

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Differential expression of growth and immunity related genes influenced by in ovo supplementation of amino acids in broiler chickens

Cited by 43 publications

References 45 publications

Effect of arginine and threonine administered in ovo on digestive organ developments and subsequent growth performance of broiler chickens

Effect of arginine and threonine administered in ovo on digestive organ developments and subsequent growth performance of broiler chickens

Effect of mannan-oligosaccharides (MOS) supplementation on performance, immunity and HSP70 gene expression in broiler chicken during hot-dry summer

Delayed post-hatch feeding affects the performance and immunocompetence differently in male and female broiler chickens

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