Differential Expression of Hox and Notch Genes in Larval and Adult Stages of Echinococcus granulosus

Dezaki, Ebrahim Saedi; Yaghoobi, Mohammad Mehdi; Taheri, Elham; Almani, Pooya Ghaseminejad; Tohidi, Farideh; Gottstein, Bruno; Harandi, Majid Fasihi

doi:10.3347/kjp.2016.54.5.653

Cited by 17 publications

(8 citation statements)

References 33 publications

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“…As an example, in the ORF strain, down-regulated genes included homeobox B7 (HoxB7) (TcrWFU_04386), which is mainly related with processes of cell differentiation and segmentation in the adult stage; since they are presented in protoscoleces and in adult worms of E. granulosus ( Dezaki et al., 2016 ). HoxB7 420 participates in processes such as cell proliferation and differentiation including 421 anterior/posterior pattern formation ( Carè et al., 1999 ).…”

Section: Discussionmentioning

confidence: 99%

The Genomes of Two Strains of Taenia crassiceps the Animal Model for the Study of Human Cysticercosis

Bobes

Estrada

Ríos-Valencia

et al. 2022

Front. Cell. Infect. Microbiol.

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Human cysticercosis by Taenia solium is the major cause of neurological illness in countries of Africa, Southeast Asia, and the Americas. Publication of four cestode genomes (T. solium, Echinococcus multilocularis, E. granulosus and Hymenolepis microstoma) in the last decade, marked the advent of novel approaches on the study of the host-parasite molecular crosstalk for cestode parasites of importance for human and animal health. Taenia crassiceps is another cestode parasite, closely related to T. solium, which has been used in numerous studies as an animal model for human cysticercosis. Therefore, characterization of the T. crassiceps genome will also contribute to the understanding of the human infection. Here, we report the genome of T. crassiceps WFU strain, reconstructed to a noncontiguous finished resolution and performed a genomic and differential expression comparison analysis against ORF strain. Both strain genomes were sequenced using Oxford Nanopore (MinION) and Illumina technologies, achieving high quality assemblies of about 107 Mb for both strains. Dotplot comparison between WFU and ORF demonstrated that both genomes were extremely similar. Additionally, karyotyping results for both strains failed to demonstrate a difference in chromosome composition. Therefore, our results strongly support the concept that the absence of scolex in the ORF strain of T. crassiceps was not the result of a chromosomal loss as proposed elsewhere. Instead, it appears to be the result of subtle and extensive differences in the regulation of gene expression. Analysis of variants between the two strains identified 2,487 sites with changes distributed in 31 of 65 scaffolds. The differential expression analysis revealed that genes related to development and morphogenesis in the ORF strain might be involved in the lack of scolex formation.

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Section: Discussionmentioning

confidence: 99%

The Genomes of Two Strains of Taenia crassiceps the Animal Model for the Study of Human Cysticercosis

Bobes

Estrada

Ríos-Valencia

et al. 2022

Front. Cell. Infect. Microbiol.

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“…5,45 . The diphasic medium was S.10E.H, consisted of two phases: (i) the liquid phase, containing 260 mL of CMRL 1066 medium (Gibco, Grand Island, NY,), 100 mL of heat-inactivated fetal calf serum (FCS, Gibco-BRL, Gaithersburg, MD), 36 mL of 5% yeast extract (Sigma-Aldrich, St. Louis, MO) in CMRL 1066, 5.6 mL of 30% glucose (Sigma-Aldrich) in distilled water, 1.4 mL of 5% dog bile in PBS, 20 mM HEPES (Sigma-Aldrich), 10 mM NaHCO3 supplemented with penicillin (100 IU/mL), streptomycin (100 mg/mL), and (ii) the solid phase that is bovine serum coagulated at 76 °C for 20–30 min 42,46 . The medium was changed every 7 days and viability and morphological development of the parasite was observed under an invert microscope (TCM 400, Labomed Inc., CA).…”

Section: Methodsmentioning

confidence: 99%

Calmodulin-specific small interfering RNA induces consistent expression suppression and morphological changes in Echinococcus granulosus

Mousavi

Afgar

Mohammadi

et al. 2019

Sci Rep

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Among parasitic helminths, biological features of Echinococcus granulosus have been a focus of particular interest in biology and medicine. The determinants and underlying molecular mechanisms of Echinococcus development in different host settings is largely unknown. The phenomenal bi-directional development of E. granulosus protoscoleces into multi-proglottid and/or microcysts, is a fascinating feature of the parasite cultivation. Calmodulin (CaM) is the major intracellular Ca2+ binding protein in plant and animal organisms. Many Ca 2+ -related processes in the physiology of eukaryotic organisms are CaM-dependent, however little is known on the role of CaM in platyhelminths growth and development. Small interfering (si) RNA-induced manipulations of the genes involving in the parasite development is an opportunity to explore novel approaches for cystic echinococcosis (CE) prevention and management. Regarding the fundamental role of CaM in cellular function of the parasites, in this study, we investigated the molecular and morphological changes induced by siRNA on CaM in different in vitro stages of E. granulosus . Three developmental stages of the tapeworm, protoscoleces, microcysts and strobilated worms, were cultivated in vitro in mono- and di-phasic media and three delivery methods, i.e. electroporation, soaking and electro-soaking, were used for RNA interference. The level of mRNA suppression as well as the phenotypic changes of the parasites were measured. Following RNA interference, EgCaM mRNA suppressions of 65–99% were recorded in different stages of the tapeworm as compared to untreated/unrelated siRNA controls. Lower viability, growth retardation, morphological abnormalities as well as EgCaM expression suppression were documented in the parasite implying potential of siRNA technology for the prevention and management of CE.

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“…hydatid cyst. Differential expression of signaling proteins among different E. granulosus and Hymenolepis microstoma developmental stages has been previously demonstrated [ 77 , 78 ]. In E. multilocularis and H. microstoma , Wnt protein expression patterns during larval metamorphosis have been elucidated [ 79 ].…”

Section: Discussionmentioning

confidence: 99%

Proteomic profiling of hydatid fluid from pulmonary cystic echinococcosis

et al. 2022

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Background Most cystic echinococcosis cases in Southern Brazil are caused by Echinococcus granulosus and Echinococcus ortleppi. Proteomic studies of helminths have increased our knowledge about the molecular survival strategies that are used by parasites. Here, we surveyed the protein content of the hydatid fluid compartment in E. granulosus and E. ortleppi pulmonary bovine cysts to better describe and compare their molecular arsenal at the host-parasite interface. Methods Hydatid fluid samples from three isolates of each species were analyzed using mass spectrometry-based proteomics (LC-MS/MS). In silico functional analyses of the identified proteins were performed to examine parasite survival strategies. Results The identified hydatid fluid protein profiles showed a predominance of parasite proteins compared to host proteins that infiltrate the cysts. We identified 280 parasitic proteins from E. granulosus and 251 from E. ortleppi, including 52 parasitic proteins that were common to all hydatid fluid samples. The in silico functional analysis revealed important molecular functions and processes that are active in pulmonary cystic echinococcosis, such as adhesion, extracellular structures organization, development regulation, signaling transduction, and enzyme activity. Conclusions The protein profiles described here provide evidence of important mechanisms related to basic cellular processes and functions that act at the host-parasite interface in cystic echinococcosis. The molecular tools used by E. granulosus and E. ortleppi for survival within the host are potential targets for new therapeutic approaches to treat cystic echinococcosis and other larval cestodiases. Graphical Abstract

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Differential Expression of Hox and Notch Genes in Larval and Adult Stages of Echinococcus granulosus

Cited by 17 publications

References 33 publications

The Genomes of Two Strains of Taenia crassiceps the Animal Model for the Study of Human Cysticercosis

The Genomes of Two Strains of Taenia crassiceps the Animal Model for the Study of Human Cysticercosis

Calmodulin-specific small interfering RNA induces consistent expression suppression and morphological changes in Echinococcus granulosus

Proteomic profiling of hydatid fluid from pulmonary cystic echinococcosis

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