Differential expression of insulin‐like growth factor binding protein 1 and ferritin light polypeptide in gestational trophoblastic neoplasia

Feng, Huichen; Tsao, Sai‐Wah; Ngan, Hextan Y.S.; Xue, Weicheng; Chiu, Pei-Chen; Cheung, Annie N.Y.

doi:10.1002/cncr.21483

Cited by 9 publications

(13 citation statements)

References 38 publications

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“…Evolved from the suppressive polymerase chain reaction (PCR) technique, SSH has a number of advantages, including ease, specificity, and the capacity to isolate low copy transcripts [10]. SSH has been successfully employed in studies of developmental biology, cancer, and immune regulation [11,12,13]. We report here the identification of a large number of genes differentially expressed during postnatal kidney development.…”

Section: Introductionmentioning

confidence: 99%

Suppression Subtractive Hybridization Analysis of Gene Expression during Late Kidney Development Identifies the Developmentally Regulated Gene <i>rPEA3</i>

Chen

Huang

et al. 2009

Nephron Exp Nephrol

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Background: While early kidney development has been studied exhaustively, the later stages of nephrogenesis that occur after birth in the rodent are relatively poorly understood. To gain insight into this process, we detected the alterations in gene expression in rat kidney at two postnatal stages, P0 (0 day after birth), the time at which nephrogensis is still active, and P21 (21 days after birth), when nephrogenesis is complete. Methods: Sprague-Dawley rats were mated, and appearance of a vaginal plug was designated as E0. Kidneys were dissected from embryos at E13, E15, E17 and E19, and from postnatal days P0, P7, P14, P21 and adult rats. Suppression subtractive hybridization (SSH) analysis was performed and highly expressed genes were evaluated as molecular markers by real-time reverse transcription-polymerase chain reaction (RT-PCR), in situ hybridization, immunofluorescence, and Western blot. Results: Several differentially expressed genes were identified, including rPEA3, a member of the PEA3 subfamily of Ets domain transcription factors. Real time RT-PCR analysis revealed that rPEA3 exhibited dynamic developmental regulation, with high levels of expression from embryonic day E15 until birth, and declining levels thereafter. By in situ hybridization, rPEA3 mRNA was detected in the ureteric bud (UB) and surrounding metanephric mesenchyme of the kidneys from E15 until birth, but was undetectable in mature kidneys. Double-immunofluorescence staining showed that both rPEA3 and WT1 expressed in the condensed mesenchymal cells at E15 and E17; and later from E19 to P7, both expressed in the epithelial cells of ureteric bud and their branches. Conclusions: These studies provide compelling evidence that SSH is an effective method for identifying genes that are regulated during renal development, and suggest that the newly identified gene rPEA3 may play an important role in kidney development and differentiation.

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Section: Introductionmentioning

confidence: 99%

Suppression Subtractive Hybridization Analysis of Gene Expression during Late Kidney Development Identifies the Developmentally Regulated Gene <i>rPEA3</i>

Chen

Huang

et al. 2009

Nephron Exp Nephrol

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“…Recently, FTL was reported to be downregulated in gestational trophoblastic neoplasia. 32 However, so far neither FTL nor RPL18 nor ZIN have been implicated in glioma pathogenesis. The same applies for the spermidine synthase gene SRM, whose transcript levels were also lower in 1p/19q-deleted tumors.…”

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confidence: 99%

Identification of novel oligodendroglioma‐associated candidate tumor suppressor genes in 1p36 and 19q13 using microarray‐based expression profiling

Tews

Felsberg

Hartmann

et al. 2006

Intl Journal of Cancer

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Loss of heterozygosity (LOH) on chromosomal arms 1p and 19q is the most common genetic alteration in oligodendroglial tumors and associated with response to radio-and chemotherapy as well as favorable prognosis. Using microsatellite analysis, we previously identified the chromosomal regions 1p36. 22-p36.31 and 19q13.3, as candidate tumor suppressor gene regions being commonly deleted in these tumors. To identify genes within these regions that are downregulated in oligodendroglial tumors with LOH 1p/19q, we performed cDNA microarray-based RNA expression profiling of 35 gliomas with known allelic status on 1p and 19q, including 7 oligodendrogliomas and 8 diffuse astrocytomas of World Health Organization (WHO) grade II, as well as 14 anaplastic oligodendrogliomas and 6 anaplastic oligoastrocytomas of WHO grade III. The microarrays used for expression profiling carried 7,000 gene-specific cDNAs, with complete coverage of the genes located in 1p36. 13-p36.31 and 19q13.2-q13.33. Microarray analysis identified 8 genes from these regions (MGC4399, SRM, ICMT, RPL18, FTL, ZIN, FLJ10781 and DBP), which all showed significantly lower expression in 1p/19q-deleted gliomas when compared to gliomas without 1p/19q losses. Quantitative real-time reverse transcription-PCR analyses were performed for the MGC4399, ICMT and RPL18 genes and confirmed the microarray findings. In addition, we found that the cytosolic phospholipase A2 (PLA2G4C) gene at 19q13.3 demonstrated significantly lower expression in anaplastic oligodendrogliomas (WHO grade III) when compared to well-differentiated oligodendrogliomas (WHO grade II). Taken together, our study provides a set of interesting novel candidate genes that may play important roles in the pathogenesis of oligodendroglial tumors. ' 2006 Wiley-Liss, Inc.Key words: gene expression profiling; loss of heterozygosity; DNA microarray; oligodendroglioma; tumor suppressor gene Gliomas are the most common primary brain tumors and constitute a heterogeneous group of neoplasms with respect to morphological appearance, biological behavior, genetic alterations, as well as response to therapy and clinical outcome. Astrocytic tumors are the most common gliomas, with glioblastoma multiforme being the most malignant and, at the same time, most frequent astrocytic tumor. In contrast, oligodendroglial tumors, comprising oligodendrogliomas and mixed oligoastrocytomas, are estimated to account for only 5-18% of all gliomas.1 Molecular genetic studies have revealed that the development of astrocytic and oligodendroglial gliomas is caused by distinct genetic alterations, which may be important for the refinement of glioma classification based on genomic profiling.2 The genetic hallmark of oligodendroglial tumors is the combined loss of heterozygosity (LOH) on chromosomal arms 1p and 19q (LOH 1p/19q), which is rare in astrocytic tumors but found in up to 80% of oligodendrogliomas and 50% of oligoastrocytomas.3 Importantly, LOH 1p/19q has emerged as an independent predictive marker of better response to radio-and ...

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“…Insulin-like growth factor (IGF) I and II were found to be increased in placental tissues prior onset of clinical preeclampsia and fetal growth restriction [12]. It has been reported that molar pregnancies had also high levels of IGF II, insulin receptors and insulin-like growth factor binding protein I (IGFBP-1) compared with normal placenta tissues [13][14][15]. The autocrine or paracrine actions of IGF-II and IGFBP-1 may be especially important during implantation and trophoblast invasion [16].…”

Section: Discussionmentioning

confidence: 98%

Increased insulin resistance and C-reactive protein in women with complete hydatidiform mole

Verit

Hilali

2011

Gynecological Endocrinology

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Differential expression of insulin‐like growth factor binding protein 1 and ferritin light polypeptide in gestational trophoblastic neoplasia

Cited by 9 publications

References 38 publications

Suppression Subtractive Hybridization Analysis of Gene Expression during Late Kidney Development Identifies the Developmentally Regulated Gene <i>rPEA3</i>

Suppression Subtractive Hybridization Analysis of Gene Expression during Late Kidney Development Identifies the Developmentally Regulated Gene <i>rPEA3</i>

Identification of novel oligodendroglioma‐associated candidate tumor suppressor genes in 1p36 and 19q13 using microarray‐based expression profiling

Increased insulin resistance and C-reactive protein in women with complete hydatidiform mole

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