Differential expression of matrix metalloproteinase-2 in human coronal and radicular sound and carious dentine

Toledano, Manuel; Nieto-Aguilar, Renato; Osorio, Raquel; Campos, Antonìo; Osorio, Estrella; Tay, Franklin R.; Alaminos, Miguel

doi:10.1016/j.jdent.2010.05.001

Cited by 69 publications

(62 citation statements)

References 28 publications

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“…While salivary enzymes may readily access outer, caries-infected dentin, it is unlikely that they would contribute to destruction of caries-affected dentin (Toledano et al 2010;Vidal et al 2014), in which dentinal fluid rather than saliva (due to the hydrostatic pressure working in favor of an outward flow of dentinal fluid) may be the source of increased activities. Alternatively, dentin matrix-bound enzymes may not necessarily be readily activated after simple in vitro demineralization.…”

Section: Dental Caries and Role Of Mmps In Caries Progressionmentioning

confidence: 99%

Role of Dentin MMPs in Caries Progression and Bond Stability

et al. 2014

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Dentin can be described as a biological composite with collagen matrix embedded with nanosized hydroxyapatite mineral crystallites. Matrix metalloproteinases (MMPs) and cysteine cathepsins are families of endopeptidases. Enzymes of both families are present in dentin and collectively capable of degrading virtually all extracellular matrix components. This review describes these enzymes and their presence in dentin, mainly focusing on their role in dentin caries pathogenesis and loss of collagen in the adhesive hybrid layer under composite restorations. MMPs and cysteine cathepsins present in saliva, mineralized dentin, and/or dentinal fluid may affect the dentin caries process at the early phases of demineralization. Changes in collagen and noncollagenous protein structure may participate in observed decreases in mechanical properties of caries-affected dentin and reduce the ability of caries-affected dentin to remineralize. These endogenous enzymes also remain entrapped within the hybrid layer during the resin infiltration process, and the acidic bonding agents themselves (irrespective of whether they are etch-and-rinse or self-etch) can activate these endogenous protease proforms. Since resin impregnation is frequently incomplete, denuded collagen matrices associated with free water (which serves as a collagen cleavage reagent for these endogenous hydrolase enzymes) can be enzymatically disrupted, finally contributing to the degradation of the hybrid layer. There are multiple in vitro and in vivo reports showing that the longevity of the adhesive interface is increased when nonspecific enzyme-inhibiting strategies are used. Different chemicals (i.e., chlorhexidine, galardin, and benzalkonium chloride) or collagen cross-linker agents have been successfully employed as therapeutic primers in the bonding procedure. In addition, the incorporation of enzyme inhibitors (i.e., quaternary ammonium methacrylates) into the resin blends has been recently promoted. This review will describe MMP functions in caries and hybrid layer degradation and explore the potential therapeutic role of MMP inhibitors for the development of improved intervention strategies for MMP-related oral diseases.

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Section: Dental Caries and Role Of Mmps In Caries Progressionmentioning

confidence: 99%

Role of Dentin MMPs in Caries Progression and Bond Stability

et al. 2014

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“…Caries also tends to increase the level of endogenous MMP-2 synthesis. 28 Acidic pH during the carious process may both induce MMP production by the odontoblasts as well as their activation, thereby potentiating MMP proteolytic capacity, leading to enhanced dentine matrix degradation. MMP-2, MMP-20 and cathepsin B present in dentinal fluid further contributes to peritubular dentine degradation.…”

Section: Role Of Mmps In Dental Cariesmentioning

confidence: 99%

Role of matrix metalloproteinases in dental caries, pulp and periapical inflammation: An overview

Jain¹,

Bahuguna²

2015

Journal of Oral Biology and Craniofacial Research

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“…1,[3][4][5] Collagen fibrils that are not encased by monomers during the bonding procedure, 6,7 as well as those exposed by the polymer degradation over time, are highly susceptible to enzymatic hydrolysis. [8][9][10][11][12] Matrix metalloproteinases (MMPs) and cysteine cathepsins have been identified in sound and caries affected-dentin 8,[12][13][14][15] and are allegedly responsible for the disappearance of portions of the hybrid layers. 11,16,17 Thus, improving collagen's resistance against enzymatic degradation and inactivating these proteases are important steps to enhance the quality and longevity of dentin bonding.…”

Section: Introductionmentioning

confidence: 99%

Transdentinal Cytotoxicity of Carbodiimide (EDC) and Glutaraldehyde on Odontoblast-like Cells

Scheffel

Bianchi

Soares

et al. 2015

Operative Dentistry

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Carbodiimide (EDC) and glutaraldehyde are cross-linking agents capable of increasing resin-dentin bond durability. This study showed that these substances might be safely applied on acid-etched dentin since they have no transdentinal cytotoxic effects on cultured odontoblast-like cells. SUMMARYObjective: To evaluate the transdentinal cytotoxicity of three different concentrations of carbodiimide (EDC) or 5% glutaraldehyde (GA) on MDPC-23 cells.Methods: Seventy 0.4-mm-thick dentin disks obtained from human molars were adapted to artificial pulp chambers. MDPC-23 cells were seeded on the pulpal surface of the disks. After 48 hours, the occlusal dentin was acid-etched and treated for 60 seconds with one of the Results: EDC at all test concentrations did not reduce cell viability, while 5% GA did increase cell metabolism. Cell death by necrosis was not elicited by EDC or 5% GA. At the 24-hour period, 0.3 M and 0.5 M EDC reduced TP production by 18% and 36.8%, respectively. At seven days, increased TP production was observed in all groups. Collagen production at the 24-hour period was reduced when 0.5 M EDC was used. After seven days, no difference was observed among the groups. SEM showed no alteration in cell morphology or number, except in the hydrogen peroxide group.Conclusions: Treatment of acid-etched dentin with EDC or GA did not cause transdentinal cytotoxic effects on odontoblast-like cells.

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Differential expression of matrix metalloproteinase-2 in human coronal and radicular sound and carious dentine

Cited by 69 publications

References 28 publications

Role of Dentin MMPs in Caries Progression and Bond Stability

Role of Dentin MMPs in Caries Progression and Bond Stability

Role of matrix metalloproteinases in dental caries, pulp and periapical inflammation: An overview

Transdentinal Cytotoxicity of Carbodiimide (EDC) and Glutaraldehyde on Odontoblast-like Cells

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