Differential expression of microRNA species in a freeze tolerant insect, Eurosta solidaginis

Courteau, Lynn; Storey, Kenneth B.; Morin, Pier Jr

doi:10.1016/j.cryobiol.2012.06.005

Cited by 53 publications

(34 citation statements)

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“…To build upon this family of cold-modulated miRNAs, the present work reports the amplification of four miRNAs with functions linked to key signaling pathways involved in cell growth and metabolism (miR-8, miR-9, miR-92b and miR-277), analyzing the responses of these miRNAs to freezing or anoxia exposures in E. solidaginis. MiR-8 and miR-92b can regulate key targets in the PTEN/PI3K/Akt pathway and influence cell metabolism in fly and mammalian models [19,31], whereas miR-9 and miR-277 have been shown to be differentially expressed in selected hypometabolic models [7,9]. This study reports, in frozen and anoxic E. solidaginis, the differential expression of miR-92b, the latest miRNA to join the growing list of CryomiRs.…”

Section: Introductionmentioning

confidence: 77%

“…These small RNA molecules are differentially regulated in a host of species that utilize hypometabolism as a stress survival strategy, including the hibernating ground squirrel Ictidomys tridecemlineatus [27], the aestivating sea cucumber Apostichopus japonicus [7], the freeze tolerant wood frog R. sylvatica [3], the intertidal snail Littorina littorea [5], and two cold-hardy gall forming insects including the goldenrod gall fly E. solidaginis [9,32]. Cold acclimation and freeze tolerance is a well-documented event in E. solidaginis [46].…”

Section: Discussionmentioning

confidence: 99%

“…Differential miRNA expression has been reported in various models of hypometabolism including hibernating mammals [37], freeze tolerant frogs [3], and cold-hardy insects [9,32]. Mir-1, capable of targeting a large number of transcript targets including histone deacetylases (HDACs) and Hsp60, is a hallmark example of an over-expressed miRNA in cold-stressed E. solidaginis larvae [32].…”

Section: Introductionmentioning

confidence: 99%

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Expression of miRNAs in response to freezing and anoxia stresses in the freeze tolerant fly Eurosta solidaginis

2015

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Section: Introductionmentioning

confidence: 77%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Expression of miRNAs in response to freezing and anoxia stresses in the freeze tolerant fly Eurosta solidaginis

2015

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“…Highlighting the role of miRNAs in the regulation of the hypometabolic state, studies have documented stress-responsive miRNA expression during mammalian hibernation (Morin et al, 2008;Kornfeld et al, 2012;Maistrovski et al, 2012;Biggar and Storey, 2014a), frog and insect freeze tolerance (Biggar et al, 2009;Courteau et al, 2012) and turtle and marine snail anoxia tolerance (Table 1) . These studies collectively indicate a conserved principle of miRNA stressresponse across phylogeny.…”

Section: јmentioning

confidence: 99%

“…However, it must be noted that this high degree of mature miRNA conservation is primarily restricted to vertebrate organisms, and invertebrate miRNA studies require further optimization given the limited information and high variability of miRNA sequences . Although the detection and quantification of invertebrate miRNAs is typically more difficult than for vertebrates, it has been carried out in both freeze-tolerant intertidal snails (Littorina littorea) and in cold-hardy insects (Epiblema scudderiana and Eurosta solidaginis) Courteau et al, 2012;Lyons et al, 2013). let-7a, miR-124, miR-1b, miR-2010, miR-2013, miR-252a, miR-716b, miR-71a, miR-73b, miR-79, miR-9, miR-92 Down: miR-100, miR-133a/b/c/d, miR-222, miR-29b, miR-33a Chen and Storey, 2014 MicroRNA regulation during stress adaptation…”

Section: јmentioning

confidence: 99%

Insight into post-transcriptional gene regulation: stress-responsive microRNAs and their role in the environmental stress survival of tolerant animals

Biggar

Storey

2015

Journal of Experimental Biology

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Living animals are constantly faced with various environmental stresses that challenge normal life, including: oxygen limitation, very low or high temperature, as well as restriction of water and food. It has been well established that in response to these stresses, tolerant organisms regularly respond with a distinct suite of cellular modifications that involve transcriptional, translational and posttranslational modification. In recent years, a new mechanism of rapid and reversible transcriptome regulation, via the action of noncoding RNA molecules, has emerged into post-transcriptional regulation and has since been shown to be part of the survival response. However, these RNA-based mechanisms by which tolerant organisms respond to stressed conditions are not well understood. Recent studies have begun to show that non-coding RNAs control gene expression and translation of mRNA to protein, and can also have regulatory influence over major cellular processes. For example, select microRNAs have been shown to have regulatory influence over the cell cycle, apoptosis, signal transduction, muscle atrophy and fatty acid metabolism during periods of environmental stress. As we are on the verge of dissecting the roles of non-coding RNA in environmental stress adaptation, this Commentary summarizes the hallmark alterations in microRNA expression that facilitate stress survival.

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Molecular Biology of Freezing Tolerance

Storey

2013

Comprehensive Physiology

163

149

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Winter survival for many kinds of animals involves freeze tolerance, the ability to endure the conversion of about 65% of total body water into extracellular ice and the consequences that freezing imposes including interruption of vital processes (e.g., heartbeat and breathing), cell shrinkage, elevated osmolality, anoxia/ischemia, and potential physical damage from ice. Freeze-tolerant animals include various terrestrially hibernating amphibians and reptiles, many species of insects, and numerous other invertebrates inhabiting both terrestrial and intertidal environments. Well-known strategies of freezing survival include accumulation of low molecular mass carbohydrate cryoprotectants (e.g., glycerol), use of ice nucleating agents/proteins for controlled triggering of ice growth and of antifreeze proteins that inhibit ice recrystallization, and good tolerance of anoxia and dehydration. The present article focuses on more recent advances in our knowledge of the genes and proteins that support freeze tolerance and the metabolic regulatory mechanisms involved. Important roles have been identified for aquaporins and transmembrane channels that move cryoprotectants, heat shock proteins and other chaperones, antioxidant defenses, and metabolic rate depression. Genome and proteome screening has revealed many new potential targets that respond to freezing, in particular implicating cytoskeleton remodeling as a necessary facet of low temperature and/or cell volume adaptation. Key regulatory mechanisms include reversible phosphorylation control of metabolic enzymes and microRNA control of gene transcript expression. These help to remodel metabolism to preserve core functions while suppressing energy expensive metabolic activities such as the cell cycle. All of these advances are providing a much more complete picture of life in the frozen state.

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Differential expression of microRNA species in a freeze tolerant insect, Eurosta solidaginis

Cited by 53 publications

References 35 publications

Expression of miRNAs in response to freezing and anoxia stresses in the freeze tolerant fly Eurosta solidaginis

Expression of miRNAs in response to freezing and anoxia stresses in the freeze tolerant fly Eurosta solidaginis

Insight into post-transcriptional gene regulation: stress-responsive microRNAs and their role in the environmental stress survival of tolerant animals

Molecular Biology of Freezing Tolerance

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