Differential expression of neuronal calcium sensor‐1 in the developing chick retina

Bergmann, Michèle; Grabs, Detlev; Roder, John; Rager, Günter; Jeromin, Andreas

doi:10.1002/cne.10302

Cited by 19 publications

(11 citation statements)

References 47 publications

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“…Therefore, the subcellular localization of NCS-1 revealed by the present study suggests that NCS-1 participates in many aspects of neuronal activity, including neurotransmission and synaptogenesis, in the developing rat spinal cord, as it appeared in neuronal cell bodies, dendrites, axons, and presynaptic terminals. This finding is in agreement with the previous studies showing the localization of NCS-1 in both the pre-and postsynaptic sites (Olafsson et al, 1997;Martone et al, 1999;Bergmann et al, 2002;Jinno et al, 2002).…”

Section: Discussionsupporting

confidence: 95%

Spatiotemporal distribution of neuronal calcium sensor‐1 in the developing rat spinal cord

Kawasaki

Nishio

Kurosawa

et al. 2003

J of Comparative Neurology

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The present study revealed the localization of neuronal calcium sensor (NCS)-1 immunoreactivity (IR) in the developing rat spinal cord. The NCS-1 IR first appeared at embryonic day 12 in the peripheral nerves and their somata. Intense NCS-1 IR was expressed in ascending and descending tracts in the white matter during the late prenatal period, which gradually decreased to the faint level during postnatal development. Intense NCS-1 IR was colocalized with growth associated protein (GAP)-43 IR in the marginal zone and with the glutamate-aspartate transporter (GLAST) IR in the radial processes traversing the marginal zone. In the adult rat white matter, radially oriented astrocytes and astrocytes in the glia limitans were double-labeled for NCS-1 and glial fibrillary acidic protein (GFAP), whereas small dots on finger-like dendritic projections were double-labeled for NCS-1 and synaptophysin. In the developing gray matter, the NCS-1 IR appeared at embryonic day 12 and gradually increased in the neuronal somata and neuropil, reaching a plateau after the end of the 4th postnatal week. The small dots in neuropil were colabeled for NCS-1 and GFAP or NCS-1 and synaptophysin in the adult rat gray matter. These results strongly suggest that NCS-1 is involved in axogenesis and synaptogenesis in the developing rat spinal cord. NCS-1 can serve as a Ca(2+)-sensor not only in neurons but also in radial glial cells or even in radially oriented astrocytes in the adult rat spinal cord.

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Section: Discussionsupporting

confidence: 95%

Spatiotemporal distribution of neuronal calcium sensor‐1 in the developing rat spinal cord

Kawasaki

Nishio

Kurosawa

et al. 2003

J of Comparative Neurology

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“…Recent studies have shown that NCS-1 is expressed within neurons of the developing and adult mouse inner ear (Sage et al, 2000), and that NCS-1 mediates survival of neurons within the avian cochlear nucleus (Wilkinson et al, 2003). Based on the pattern of NCS-1 expression, it has been suggested that NCS-1 may be involved in axogenesis and synaptogenesis (Reynolds et al, 2001;Bergmann et al, 2002;Kawaski et al, 2003). We examined apoptosis-mediated cell death in the developing otocysts of ncs-1a morphant embryos using a whole-mount TUNEL assay and found very little cell death in the ncs-1a morphant embryos and their wild-type clutch-mates (data not shown).…”

Section: Discussionmentioning

confidence: 95%

Neuronal calcium sensor-1 genencs-1a is essential for semicircular canal formation in zebrafish inner ear

et al. 2005

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We have analyzed the functional role of neuronal calcium sensor-1 (Ncs-1) in zebrafish development. We identified two orthologs of the mammalian NCS-1 gene. Full-length cDNAs encoding zebrafish Ncs-1a and Ncs-1b polypeptides were cloned and characterized. Whole-mount in situ hybridization revealed that ncs-1a mRNA was expressed beginning at early somitogenesis. As development progressed, ncs-1a mRNA was present throughout the embryo with expression detected in ventral hematopoietic mesoderm, pronephric tubules, CNS nuclei, and otic vesicle. By 4.5 days post fertilization (dpf), ncs-1a expression was detected primarily in the brain. Expression of ncs-1b mRNA was first detected at 36 hours post fertilization (hpf) and was restricted to the olfactory bulb. By 4.5 dpf, ncs-1b was expressed at low levels throughout the brain. Knockdown of ncs-1a mRNA translation with antisense morpholinos blocked formation of semicircular canals. These studies identify a novel function for ncs-1a in inner ear development and suggest that this calcium sensor plays an important role in vestibular function.

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“…layer at E5, before ganglion cell synapses in either the retina or tectum (Bergmann et al, 2002). Similarly, in the developing mouse inner ear, NCS-1 is in the vestibular and cochlear afferents before synapse formation (Sage et al, 2000).…”

Section: Ncs-1 and Axon Outgrowthmentioning

confidence: 98%

Expression of the neuronal calcium sensor protein NCS‐1 in the developing mouse olfactory pathway

Treloar

Uboha

Jeromin

et al. 2004

J of Comparative Neurology

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Neuron specific calcium sensor 1 (NCS-1) is widely expressed in the developing and adult nervous system. Like calmodulin, NCS-1 is a member of a family of calcium binding proteins that contain EF-hand motifs, which bind calcium and induce conformational changes in the protein. Their binding varies with calcium concentration, allowing them to act as true calcium sensors rather than just calcium binding proteins. This family of proteins has been implicated in important synaptic events including neurotransmitter release and synapse formation. We examined the expression of NCS-1 in the developing and mature olfactory system to determine whether this molecule may be playing a role in establishing and/or maintaining olfactory circuitry. During development, expression of NCS-1 in the olfactory epithelium was localized in the dendritic knobs and axons of olfactory sensory neurons. Axonal expression was down-regulated after synapse formation. In the developing olfactory bulb, NCS-1 was expressed in the processes of mitral/tufted and granule cells. However, in the adult olfactory bulb, strongest expression was found in a subset of periglomerular cells (PGCs). This subset of PGCs did not express other known markers of PGCs including tyrosine hydroxylase, glutamic acid decarboxylase, calbindin, or calretinin, and only partially overlapped with the subpopulation of PGCs that express parvalbumin. Together, these data suggest multiple and overlapping roles of NCS-1 in the developing and mature olfactory system.

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Differential expression of neuronal calcium sensor‐1 in the developing chick retina

Cited by 19 publications

References 47 publications

Spatiotemporal distribution of neuronal calcium sensor‐1 in the developing rat spinal cord

Spatiotemporal distribution of neuronal calcium sensor‐1 in the developing rat spinal cord

Neuronal calcium sensor-1 genencs-1a is essential for semicircular canal formation in zebrafish inner ear

Expression of the neuronal calcium sensor protein NCS‐1 in the developing mouse olfactory pathway

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