Differential expression of six genes and correlation with fatness traits in a unique broiler population

Jin, Pengcheng; Wu, Xianwen; Xu, Song-Song; Zhang, Hui; Li, Yumao; Zhi, Cao; Li, Hui; Wang, Shouzhi

doi:10.1016/j.sjbs.2015.04.014

Cited by 24 publications

(20 citation statements)

References 32 publications

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“…Obesity causes adverse health outcomes in humans, and similar problems exist in chickens ( Jin et al , 2017 ). Excessive abdominal fat accumulates in chickens, making them an ideal model organism for studying obesity-associated metabolic changes.…”

Section: Discussionmentioning

confidence: 99%

Effect of Vitamin E Supplementation on Deposition and Gene Expression Profiling of Abdominal Fat in Broiler Chickens

Zhang

Zhu

et al. 2021

J. Poult. Sci.

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The aim of this study was to study the regulation of abdominal fat deposition by DL- α -tocopherol acetate (vitamin E) in broilers. Diets supplemented with 50 IU vitamin E significantly diminished abdominal fat deposition in broilers at day 35. Transcriptome sequencing results for abdominal fat tissues of the control (FC) and 50 IU vitamin E-supplemented (FT) groups identified 602 differentially expressed genes (DEGs), which were enriched in cellular process, cell and cell part, and binding Gene Ontology terms. Pathway functional analysis revealed that the DEGs were enriched in 42 metabolic pathways. Notably, the most enriched pathway, fatty acid biosynthesis, was found to play a key role in lipid metabolism. Further, the key regulators of lipid metabolism, including fatty acid synthase, acetyl-CoA carboxylase alpha, and acyl-CoA synthetase long-chain family member 1, demonstrated decreased expression following vitamin E supplementation. Herein, we have identified pathways and genes regulated by vitamin E, thereby providing novel insights into the nutrients regulating abdominal fat deposition in broilers.

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Section: Discussionmentioning

confidence: 99%

Effect of Vitamin E Supplementation on Deposition and Gene Expression Profiling of Abdominal Fat in Broiler Chickens

Zhang

Zhu

et al. 2021

J. Poult. Sci.

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“…However, the genetic and molecular mechanisms that link to obesity are largely not known. The genes or molecules that regulating abdominal fat deposition or abdominal adipose tissue development can be identified by many different genomic approaches (Tatsuda and Fujinaka, 2001 ; Abasht and Lamont, 2007 ; Huang et al, 2015a ; Ouyang et al, 2016 ; Jin et al, 2017 ; Zhang et al, 2017 ). Comparison studies between lean and fat chickens in abdominal fat can provide useful information about the underlying genetic variation in the trait.…”

Section: Introductionmentioning

confidence: 99%

“…Recently, Jin et al ( 2017 ) investigated the expression of 6 top candidate genes [steroid 5 alpha-reductase 3 (SRD5A3), sarcoglycan zeta (SGCZ), DLC1 Rho GTPase activating protein (DLC1), 1,4-alpha-glucan branching enzyme 1 (GBE1), polypeptide N-acetylgalactosaminyltransferase 9 (GALNT9), and DnaJ heat shock protein family (Hsp40) member B6 (DNAJB6)] obtained from GWAS result (unpublished data) in abdominal fat between fat and lean broilers of NEAUHLF population. SRD5A3 and SGCZ expressions were significantly positively associated with AFW and AFP.…”

Section: Introductionmentioning

confidence: 99%

Genomic Insights Into the Multiple Factors Controlling Abdominal Fat Deposition in a Chicken Model

Abdalla

Chen²,

Nie³

et al. 2018

Front. Genet.

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Genetic selection for an increased growth rate in meat-type chickens has been accompanied by excessive fat accumulation particularly in abdominal cavity. These progressed to indirect and often unhealthy effects on meat quality properties and increased feed cost. Advances in genomics technology over recent years have led to the surprising discoveries that the genome is more complex than previously thought. Studies have identified multiple-genetic factors associated with abdominal fat deposition. Meanwhile, the obesity epidemic has focused attention on adipose tissue and the development of adipocytes. The aim of this review is to summarize the current understanding of genetic/epigenetic factors associated with abdominal fat deposition, or as it relates to the proliferation and differentiation of preadipocytes in chicken. The results discussed here have been identified by different genomic approaches, such as QTL-based studies, the candidate gene approach, epistatic interaction, copy number variation, single-nucleotide polymorphism screening, selection signature analysis, genome-wide association studies, RNA sequencing, and bisulfite sequencing. The studies mentioned in this review have described multiple-genetic factors involved in an abdominal fat deposition. Therefore, it is inevitable to further study the multiple-genetic factors in-depth to develop novel molecular markers or potential targets, which will provide promising applications for reducing abdominal fat deposition in meat-type chicken.

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“…N-acetylgalactosaminyltransferase 9 ( GALNT9 ) encodes a member of the polypeptide N-acetylgalactosaminy-ltransferase family of enzymes 51 that participates in the biosynthesis of O polyose and has been found to play an important role in glycobiology 52 . In oviductal epithelial cells, proteins can be glycosylated, resulting in the formation of glycoproteins that are stored in the form of albumen in eggs.…”

Section: Discussionmentioning

confidence: 99%

RNA-Seq reveals seven promising candidate genes affecting the proportion of thick egg albumen in layer-type chickens

Wan

Jin

et al. 2017

Sci Rep

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Eggs with a much higher proportion of thick albumen are preferred in the layer industry, as they are favoured by consumers. However, the genetic factors affecting the thick egg albumen trait have not been elucidated. Using RNA sequencing, we explored the magnum transcriptome in 9 Rhode Island white layers: four layers with phenotypes of extremely high ratios of thick to thin albumen (high thick albumen, HTA) and five with extremely low ratios (low thick albumen, LTA). A total of 220 genes were differentially expressed, among which 150 genes were up-regulated and 70 were down-regulated in the HTA group compared with the LTA group. Gene Ontology (GO) analysis revealed that the up-regulated genes in HTA were mainly involved in a wide range of regulatory functions. In addition, a large number of these genes were related to glycosphingolipid biosynthesis, focal adhesion, ECM-receptor interactions and cytokine-cytokine receptor interactions. Based on functional analysis, ST3GAL4, FUT4, ITGA2, SDC3, PRLR, CDH4 and GALNT9 were identified as promising candidate genes for thick albumen synthesis and metabolism during egg formation. These results provide new insights into the molecular mechanisms of egg albumen traits and may contribute to future breeding strategies that optimise the proportion of thick egg albumen.

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Differential expression of six genes and correlation with fatness traits in a unique broiler population

Cited by 24 publications

References 32 publications

Effect of Vitamin E Supplementation on Deposition and Gene Expression Profiling of Abdominal Fat in Broiler Chickens

Effect of Vitamin E Supplementation on Deposition and Gene Expression Profiling of Abdominal Fat in Broiler Chickens

Genomic Insights Into the Multiple Factors Controlling Abdominal Fat Deposition in a Chicken Model

RNA-Seq reveals seven promising candidate genes affecting the proportion of thick egg albumen in layer-type chickens

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