Differential expression of two cytochrome P450 genes in compatible and incompatible Hessian fly/wheat interactions

Mittapalli, Omprakash; Neal, Jonathan J.; Shukle, Richard H.

doi:10.1016/j.ibmb.2005.03.011

Cited by 23 publications

(26 citation statements)

References 38 publications

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“…Larval tissues including midgut, salivary glands, and fat body were dissected as described earlier (24). RNA was isolated from the larval tissues and different stages of development (first, second, and early third larval instars, pupae, and adults) using the RNAqueous-4PCR kit from Ambion (Austin, TX).…”

Section: Methodsmentioning

confidence: 99%

“…RNA was isolated from the larval tissues and different stages of development (first, second, and early third larval instars, pupae, and adults) using the RNAqueous-4PCR kit from Ambion (Austin, TX). RNA extracted from 200 midguts was used to construct a cDNA library using a Smart cDNA library construction kit from Clontech (Mountain View, CA) as described earlier (24).…”

Section: Methodsmentioning

confidence: 99%

“…The fold change during development was calculated by taking the expression level of the first larval instar as the calibrator. The fold change in antioxidant gene mRNA levels during compatible and incompatible interactions was assessed by dividing the REV for larvae on resistant plants by the REV for larvae on susceptible plants for all of the seven times points examined (6,12,18,24,48,72, and 96 h after hatching). The standard error represented the variance in three technical replicates for the tissue/development expression analysis and two biological replicates (two technical replicates within each) for the interaction study.…”

Section: Methodsmentioning

confidence: 99%

“…These studies suggest a plausible role for two delta GSTs (MdesGST-1 and MdesGST-3) and a CYP6 cytochrome P450 (CYP6AZ1) in detoxifying wheat allelochemicals during feeding. Furthermore, a sigma GST (MdesGST-2) and another CYP6 cytochrome P450 (CYP6BA1) were speculated to have general functions during development (23,24). In this study we report the transcription profiles of two PHGPXs (MdesPHGPX-1 and MdesPHGPX-2), two CATs (MdesCAT-1 and MdesCAT-2), and two SODs (MdesSOD-1 and MdesSOD-2) in larval tissues during development and in larvae participating in compatible and incompatible interactions.…”

mentioning

confidence: 97%

“…The presence of glutathione-S-transferases (GSTs) (23) and cytochrome P450s (24) has been documented in the Hessian fly. These studies suggest a plausible role for two delta GSTs (MdesGST-1 and MdesGST-3) and a CYP6 cytochrome P450 (CYP6AZ1) in detoxifying wheat allelochemicals during feeding.…”

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confidence: 99%

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Antioxidant defense response in a galling insect

Mittapalli¹,

Neal²,

Shukle

2007

Proc. Natl. Acad. Sci. U.S.A.

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114

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Herbivorous insect species are constantly challenged with reactive oxygen species (ROS) generated from endogenous and exogenous sources. ROS produced within insects because of stress and prooxidant allelochemicals produced by host plants in response to herbivory require a complex mode of antioxidant defense during insect/plant interactions. Some insect herbivores have a midgutbased defense against the suite of ROS encountered. Because the Hessian fly (Mayetiola destructor) is the major insect pest of wheat worldwide, and an emerging model for all gall midges, we investigated its antioxidant responses during interaction with its host plant. Quantitative data for two phospholipid glutathione peroxidases (MdesPHGPX-1 and MdesPHGPX-2), two catalases (MdesCAT-1 and MdesCAT-2), and two superoxide dismutases (MdesSOD-1 and MdesSOD-2) revealed high levels of all of the mRNAs in the midgut of larvae on susceptible wheat (compatible interaction). During development of the Hessian fly on susceptible wheat, a differential expression pattern was observed for all six genes. Analysis of larvae on resistant wheat (incompatible interaction) compared with larvae on susceptible wheat showed increased levels of mRNAs in larvae on resistant wheat for all of the antioxidant genes except MdesSOD-1 and MdesSOD-2. We postulate that the increased mRNA levels of MdesPHGPX-1, MdesPH-GPX-2, MdesCAT-1, and MdesCAT-2 reflect responses to ROS encountered by larvae while feeding on resistant wheat seedlings and/or ROS generated endogenously in larvae because of stress/ starvation. These results provide an opportunity to understand the cooperative antioxidant defense responses in the Hessian fly/ wheat interaction and may be applicable to other insect/plant interactions.Hessian fly ͉ insect/plant interaction ͉ reactive oxygen species ͉ wheat

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 97%

mentioning

confidence: 99%

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Antioxidant defense response in a galling insect

Mittapalli¹,

Neal²,

Shukle

2007

Proc. Natl. Acad. Sci. U.S.A.

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114

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cDNA cloning and transcriptional expression of a peritrophin‐like gene in the Hessian fly, Mayetiola destructor [Say]

Mittapalli

Sardesai

Shukle

2006

Arch Insect Biochem Physiol

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One of the well-studied components of the insect gut is the peritrophic matrix (PM). This semipermeable structure primarily functions in digestion, and protection against invasive microorganisms and mechanical damage. We report the cDNA cloning and transcription profiles of a peritrophin-A like gene (designated MdesPERI-A1) in the Hessian fly Mayetiola destructor. The predicted amino acid sequence of MdesPERI-A1 revealed a putative secretion signal peptide at its amino terminus, similarity to peritrophins from other insects including dipterans, and the presence of two chitin binding domains each containing six cysteine residues. Quantitative expression analysis of MdesPERI-A1 mRNA in different larval tissues revealed the transcript to be predominantly present in the midgut (597.9-fold) compared to other tissues assayed including salivary glands and fat bodies. Spatial expression patterns during development showed a peak expression of MdesPERI-A1 in the feeding second-instars (146-fold) and a decline in expression in the pupal and adult stages. Transcription profiling of MdesPERI-A1 during compatible (larvae on susceptible plants) and incompatible (larvae on resistant plants) interactions with wheat revealed a greater level (1.7-fold) of MdesPERI-A1 transcript in larvae on resistant plants in the initial time point examined. However, MdesPERI-A1 expression declined in larvae on resistant plants at the later time points.

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Initiation of compatible wheat-Hessian fly interactions triggers the expression of a novel UDP-glycosyltransferase, MdesUGT1, in virulent Hessian fly larvae

Subramanyam

Nemacheck

2021

Arthropod-Plant Interactions

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Insect UDP-glycosyltransferases (UGTs) play an important role in detoxification of substrates such as plant allelochemicals, and cuticle formation by the process of glucosidation. Hessian fly (Mayetiola destructor), belonging to the order Diptera (Family: Cecidomyiidae), is a destructive pest of host wheat causing significant economic losses. In the current study, using the assembled genome, we identified thirteen genes in M. destructor that belong to the family of UGTs (MdesUGT). Expression profiling revealed differential expression of MdesUGT genes in Hessian fly feeding instars. Further, we report the molecular cloning of MdesUGT1, designated as UGT301F1, from M. destructor. Characterization of the MdesUGT1 amino acid sequence revealed a conserved signature motif and sugar donor-binding domains characteristic of UGT proteins. Further expression analysis revealed dramatic increase in transcript accumulation of MdesUGT1 in the first and second feeding instars during compatible interactions (susceptible wheat, virulent larvae) but lacked significant upregulation during incompatible wheat Hessian fly interactions. Similar increase in MdesUGT1 transcripts was also observed during interactions of Hessian fly with nonhost, Brachypodium distachyon. These findings suggest the possible early involvement of MdesUGT1 in detoxification of plant toxins, and subsequent role in cuticular formation, thus contributing to the growth and development of this dipteran insect pest. Identification and characterization of insect UGTs could provide valuable insights into the detoxification and growth inhibitory mechanisms and facilitate future plant pest management strategies.

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Differential expression of two cytochrome P450 genes in compatible and incompatible Hessian fly/wheat interactions

Cited by 23 publications

References 38 publications

Antioxidant defense response in a galling insect

Antioxidant defense response in a galling insect

cDNA cloning and transcriptional expression of a peritrophin‐like gene in the Hessian fly, Mayetiola destructor [Say]

Initiation of compatible wheat-Hessian fly interactions triggers the expression of a novel UDP-glycosyltransferase, MdesUGT1, in virulent Hessian fly larvae

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