Differential expression of two galactinol synthase isoforms LcGolS1 and LcGolS2 in developing lentil ( Lens culinaris Medik. cv CDC Redberry) seeds

Kannan, Udhaya; Sharma, Roopam; Khedikar, Yogendra; Gangola, Manu P.; Ganeshan, S.; Båga, Monica; Chibbar, Ravindra N.

doi:10.1016/j.plaphy.2016.08.004

Cited by 13 publications

(3 citation statements)

References 35 publications

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“…AsA‐fed leaves had higher abundance of galactinol and myo‐inositol (spot 11, 12; Figure b; Table ). Galactinol and myo‐inositol are involved in the biosynthesis of raffinose family oligosaccharides (RFOs) (Kannan et al, ; Sengupta, Mukherjee, Basak, & Majumder, ). RFOs also serve as compatible solutes under stress conditions (Bocian et al, ; Kaplan et al, ).…”

Section: Discussionmentioning

confidence: 99%

A metabolomics study of ascorbic acid‐induced in situ freezing tolerance in spinach (Spinacia oleracea L.)

2020

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Freeze–thaw stress is one of the major environmental constraints that limit plant growth and reduce productivity and quality. Plants exhibit a variety of cellular dysfunctions following freeze–thaw stress, including accumulation of reactive oxygen species (ROS). This means that enhancement of antioxidant capacity by exogenous application of antioxidants could potentially be one of the strategies for improving freezing tolerance (FT) of plants. Exogenous application of ascorbic acid (AsA), as an antioxidant, has been shown to improve plant tolerance against abiotic stresses but its effect on FT has not been investigated. We evaluated the effect of AsA‐feeding on FT of spinach (Spinacia oleracea L.) at whole plant and excised‐leaf level, and conducted metabolite profiling of leaves before and after AsA treatment to explore metabolic explanation for change in FT. AsA application did not impede leaf growth, instead slightly promoted it. Temperature‐controlled freeze–thaw tests revealed AsA‐fed plants were more freezing tolerant as indicated by: (a) less visual damage/mortality; (b) lower ion leakage; and (c) less oxidative injury, lower abundance of free radicals (O2·- and H2O2). Comparative leaf metabolite profiling revealed clear separation of metabolic phenotypes for control versus AsA‐fed leaves. Specifically, AsA‐fed leaves had greater abundance of antioxidants (AsA, glutathione, alpha‐ & gamma‐tocopherol) and compatible solutes (proline, galactinol, and myo‐inositol). AsA‐fed leaves also had higher activity of antioxidant enzymes (superoxide dismutase, ascorbate peroxidase, and catalase). These changes, together, may improve FT via alleviating freeze‐induced oxidative stress as well as protecting membranes from freeze desiccation. Additionally, improved FT by AsA‐feeding may potentially include enhanced cell wall/lignin augmentation and bolstered secondary metabolism as indicated by diminished level of phenylalanine and increased abundance of branched amino acids, respectively.

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Section: Discussionmentioning

confidence: 99%

A metabolomics study of ascorbic acid‐induced in situ freezing tolerance in spinach (Spinacia oleracea L.)

2020

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“…AsA-fed leaves had higher abundance of galactinol and myo-inositol (spot 11, 12; Figure 5b; Table 2). Galactinol and myo-inositol are involved in the biosynthesis of raffinose family oligosaccharides (RFOs) (Sengupta, Mukherjee, Basak, & Majumder, 2015; Kannan et al, 2016). RFOs also serve as compatible solutes under stress conditions (Kaplan et al, 2004; Bocian et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

A metabolomics study of ascorbic acid-inducedin situfreezing tolerance in spinach (Spinacia oleraceaL.)

Arora

Min

Chen

2020

Preprint

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Freeze-thaw stress is one of the major environmental constraints that limit plant growth and reduces productivity and quality. Plants exhibit a variety of cellular dysfunction following freeze-thaw stress, including accumulation of reactive oxygen species (ROS). This means that enhancement of antioxidant capacity by exogenous application of antioxidants could potentially be one of major strategies for improving freezing tolerance (FT) of plants. Exogenous application of ascorbic acid (AsA), as an antioxidant, has been shown to improve plant tolerance against abiotic stresses but its effect on FT has not been investigated. We evaluated the effect of AsA-feeding on FT of spinach (Spinacia oleracea L.) at whole-plant and excised leaf level, and conducted metabolite profiling of leaves before and after AsA-treatment to explore metabolic explanation for change in FT. AsA-application did not impede leaf-growth; instead slightly promoted it. Temperature-controlled freeze-thaw tests revealed AsA-fed plants were more freezing tolerant as indicated by: 1) less visual damage/mortality; 2) lower ion-leakage; and 3) less oxidative-injury, lower abundance of free radicals (O2•− and H2O2). Comparative leaf metabolic profiling revealed clear separation of metabolic phenotypes for control vs. AsA-fed leaves. Specifically, AsA-fed leaves had greater abundance of antioxidants (AsA, glutathione, alpha-& gamma-tocopherol) and compatible solutes (proline, galactinol, myo-inositol). AsA-fed leaves also had higher activity of antioxidant enzymes (superoxide dismutase, ascorbate peroxidase, catalase). These changes, together, may improve FT via alleviating freeze-induced oxidative stress as well as protecting membranes from freeze-desiccation. Additionally, improved FT by AsA-feeding may potentially include enhanced cell wall/lignin augmentation and bolstered secondary metabolism as indicated by diminished level of phenylalanine and increased abundance of branched amino acids, respectively.

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“…In recent years, with the growing availability of information generated by genome sequencing in plants, many studies have been conducted for identifying and understanding the functional significance of inumerous genes. GolS genes have been the subject of several studies in many plant species, and are genes that are upregulated in reponse to multiple developmental and environmental responses (DOS SANTOS et al, 2015;KANNAN et al, 2016;LIU et al, 2016;JANG et al, 2018;CHU et al, 2018). In an effort to accelerate the genomic studies in banana, a double haploid (DH-Pahang) had its genome sequenced, and a platform was made available for the characterization of its genes (D'HONT et al, 2012;DROC et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

A GENOME-WIDE ANALYSIS OF THE GALACTINOL SYNTHASEGENE FAMILY IN BANANA (Musa acuminata)

Dolcimasculo¹,

Ribas²,

Vieira³

et al. 2018

COLLOQ AGRARIAE

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Galactinol synthase (GolS) is theenzyme that catalyzes the first step of the biosynthesis of the raffinose family oligosaccharides (RFOs), andisinvolved in manybiological processes in plants.In the present study, four putative GolSgenes were identified in the Musa acuminatagenome. We further characterized these MaGolSgenes in terms of protein length, molecular weight, theoretical isoelectric point and 3D protein structure. Genomic organization revealed that most MaGolSgenes have four exons. The conserved motifs were identified,demonstrating high group-specificityof all MaGolS proteins. Multiple sequence alignment showedthat theAPSAA typical domainispresent in all GolS proteins. Comparativephylogenetic analysis oftheMaGolS proteins revealed three distinct groups.These data provide insight to support new studies adressing the role of GolSgenes in this important fruit species.

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Differential expression of two galactinol synthase isoforms LcGolS1 and LcGolS2 in developing lentil ( Lens culinaris Medik. cv CDC Redberry) seeds

Cited by 13 publications

References 35 publications

A metabolomics study of ascorbic acid‐induced in situ freezing tolerance in spinach (Spinacia oleracea L.)

A metabolomics study of ascorbic acid‐induced in situ freezing tolerance in spinach (Spinacia oleracea L.)

A metabolomics study of ascorbic acid-inducedin situfreezing tolerance in spinach (Spinacia oleraceaL.)

A GENOME-WIDE ANALYSIS OF THE GALACTINOL SYNTHASEGENE FAMILY IN BANANA (Musa acuminata)

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