Differential expression of β -1,3-glucanase in susceptible and resistant melon cultivars in response to infection by Sphaerotheca fusca

Rivera, María Eugenia Sánchez Díaz de; Codina, Juan; Olea, Francisco; Vicente, Antonio de; Pérez-Garcı́a, Alejandro

doi:10.1006/pmpp.2002.0436

Cited by 24 publications

(2 citation statements)

References 37 publications

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“…McCreight & Coffey, (2011) melaporkan bahwa melon kultivar PI 312970 memiliki 3 mekanisme pertahanan, yaitu mengurangi perkecambahan konidia dan pembentukan appressorium, hypersensitive response, dan resistant blisters. Rivera, Codina, Olea, de Vicente, & Perez-Garcia, (2002) menemukan bahwa terjadi induksi awal transkripsi enzim hidolitik β-1, 3-glucanase pada kultivar melon PMR-6 yang resisten terhadap infeksi penyakit embun tepung. Enzim ini dapat mendegradasi dinding sel dan menyebabkan kematian patogen.…”

Section: Gambar 4 Buah Melon Kultivar Meloniunclassified

Identification and Analysis of Powdery Mildew Resistance in Melon (Cucumis melo L.) Cultivar Meloni

Ishak

Daryono²

2020

BES

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Background: A powdery mildew-resistant cultivar of melon is needed to increase melon yield crops. Meloni is a superior melon cultivar bred through a crossing between ♀ SL-3 and ♂ PI 371795, resulted by the Laboratory of Genetics and Breeding, Faculty of Biology, UGM. This study aimed to determine the level resistance of Meloni to powdery mildew infection and to identify the powdery mildew species that infected Meloni based on morphological characters. Methods: Meloni seeds were germinated and planted in the greenhouse of PIAT UGM. Powdery mildew spores were inoculated into the leaves after ±2 weeks of age. Leaf infected were scored using the gridline every 3 days for 6 weeks. Scoring results were converted to the diseases index score. Furthermore, powdery mildew species was identified using morphological characters. Results: Meloni had a tolerance level of resistance to the powdery mildew infection. Based on the morphological characters with fibrosin bodies, conidia ovoid-shape and the position of the germ tube in the lateral part of the conidia, powdery mildew that infected Meloni was expected as P. xanthii. Conclusions: Meloni can be expected as an alternative to superior melon seeds resistant to pest and disease infections especially powdery mildew.

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Section: Gambar 4 Buah Melon Kultivar Meloniunclassified

Identification and Analysis of Powdery Mildew Resistance in Melon (Cucumis melo L.) Cultivar Meloni

Ishak

Daryono²

2020

BES

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“…Induction of β-glucans has been demonstrated in many plant-fungal pathogen interactions and they are thought to play several roles in plant defense. Firstly, they can degrade the cell wall of pathogen or disrupt its deposition, contributing to pathogen death [11]. Secondary, they can release cell wall fragments that act as elicitors of active host defense response [12].…”

Section: Introductionmentioning

confidence: 99%

Systemic Acquired Resistance Induced by Some Biotic Agents against Downy Mildew of Cucumber Disease

2015

International Conference on Plant, Marine and Environmental Sciences (PMES-2015) Jan. 1-2, 2015 Kuala Lumpur (Malaysia)

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Systemic acquired resistance (SAR) is known to play an important role in plant disease and pest resistance. In this study the interactions between cucumber downy mildew disease caused by Pseudoperonospora cubensis and some biological control agents (Trichoderma harazianum; Pseudomonas fluorescen; Ampelomyces quisqualis) and its consequences changes on pathogenesis related (PR) proteins activity such as: Peroxidase (PO), β-1,3-glucanase (GLU) and Chitinase (CHI) were investigated. As a result of plant infection with P. cubensi, defensive responses are mediated by hypersensitivity reaction (HR), SA signal pathway and PR-proteins. The control of downy mildew on cucumber by the fungus T. harzianum under greenhouse conditions led to increase of both peroxidase and β-1, 3-glucanase activities. Such activities were used as a marker for resistance to P. cubensis. While biological treatment with P. fluorescens did not induce peroxidase activity but it was a good inducer to β-1,3-glucanase activity. On the other hand, treatment with A. quisqualis did induce neither peroxidase nor β-1,3-glucanase (Although it gave a good result in SA signal pathway induction). All bio-agents showed no or less activation for chitinase. After (HR) response plant protection extended to 7 days. These results prove that all biotic inducers have the ability to induce (SAR) against cucumber downy mildew and give higher protection.

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Identification of QTLs for resistance to powdery mildew and SSR markers diagnostic for powdery mildew resistance genes in melon (Cucumis melo L.)

Fukino

Ohara

Monforte³

et al. 2008

Theor Appl Genet

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Powdery mildew caused by Podosphaera xanthii is an important foliar disease in melon. To find molecular markers for marker-assisted selection, we constructed a genetic linkage map of melon based on a population of 93 recombinant inbred lines derived from crosses between highly resistant AR 5 and susceptible 'Earl's Favourite (Harukei 3)'. The map spans 877 cM and consists of 167 markers, comprising 157 simple sequence repeats (SSRs), 7 sequence characterized amplified region/cleavage amplified polymorphic sequence markers and 3 phenotypic markers segregating into 20 linkage groups. Among them, 37 SSRs and 6 other markers were common to previous maps. Quantitative trait locus (QTL) analysis identified two loci for resistance to powdery mildew. The effects of these QTLs varied depending on strain and plant stage. The percentage of phenotypic variance explained for resistance to the pxA strain was similar between QTLs (R (2) = 22-28%). For resistance to pxB strain, the QTL on linkage group (LG) XII was responsible for much more of the variance (41-46%) than that on LG IIA (12-13%). The QTL on LG IIA was located between two SSR markers. Using an independent population, we demonstrated the effectiveness of these markers. This is the first report of universal and effective markers linked to a gene for powdery mildew resistance in melon.

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Differential expression of β -1,3-glucanase in susceptible and resistant melon cultivars in response to infection by Sphaerotheca fusca

Cited by 24 publications

References 37 publications

Identification and Analysis of Powdery Mildew Resistance in Melon (Cucumis melo L.) Cultivar Meloni

Identification and Analysis of Powdery Mildew Resistance in Melon (Cucumis melo L.) Cultivar Meloni

Systemic Acquired Resistance Induced by Some Biotic Agents against Downy Mildew of Cucumber Disease

Identification of QTLs for resistance to powdery mildew and SSR markers diagnostic for powdery mildew resistance genes in melon (Cucumis melo L.)

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