Differential Expression Pattern of Splice Variants of Amino Acid Transporter Genes from Rice Grown under Various Nitrogen Conditions and during Development

Abstract

Amino acid transporters (AATs) play indispensable roles in nutrient uptake and allocation for plants. Alternative splicing of gene's post transcription can increase diversity of protein and allow novel ways to regulate growth and development of plant. In this study, 11 OsAAT alternative-splicing genes with 24 kinds of predicted mRNAs were identified from 85 rice AAT genes; of these, 23 alternative-splicing open reading frames were actually expressed in rice. Quantitative real time-polymerase chain reaction results showed that the predicted primary splice variant 1 was the main alternative splice variant for 8 genes because of its higher expression levels but splice variant 2 was also an important variant existing individually. The expression of splice variants in a gene was regulated by nitrogen (N) and the expression of 5 genes was up-regulated at higher concentration of all forms of N and that of nine genes was up-regulated at higher nitrate concentration. The mRNA levels of splice variants were also regulated during leaf and panicle development of rice. It indicated that alternative splice variants of OsAAT family were regulated with a natural variation in expression ratio grown under various nitrogen conditions in rice.

Stress-Induced Changes in Alternative Splicing Landscape in Rice: Functional Significance of Splice Isoforms in Stress Tolerance

“…Consistent with the results from genome-wide studies, the expression of abiotic stress-induced individual mRNA variants of several regulatory and structural genes is development- or tissue-specific [ 73 , 79 , 83 , 99 , 101 , 102 ], while the variants from other genes show differential expression in various tissues and/or at developmental stages [ 77 , 81 , 96 , 102 , 103 , 104 , 105 , 106 , 107 , 108 ]. The spatial expression of splice variants has a potential significance for rice stress tolerance.…”

Genome-wide analysis of the soybean root transcriptome reveals the impact of nitrate on alternative splicing

“…Several recent studies have explored the interactions among AS, nitrate, and root development ( Fang et al 2017 ; Ishizawa et al 2019 ; Wang et al 2020 ). It has been reported that more than 1000 maize genes have exhibited specific AS modulations in response to N treatment, which altered early N responses ( Wang et al 2020 ).…”

“…It has been reported that more than 1000 maize genes have exhibited specific AS modulations in response to N treatment, which altered early N responses ( Wang et al 2020 ). AS variants of rice amino acid transporter genes ( OsAAT s) were regulated by natural variations in expression ratios in rice grown with various levels of nitrogen; these AS variants then regulated nutrient uptake and allocation ( Fang et al 2017 ). Root hairs play important roles in water absorption and nutrient uptake, and splicing factor 3b is involved in pre-mRNA splicing, which is associated with root hair development in response to light signals ( Ishizawa et al 2019 ).…”