Differential Expression Profile and Genetic Variants of MicroRNAs Sequences in Breast Cancer Patients

Alshatwi, Ali A.; Shafi, Gowhar; Hasan, Tarique N.; Syed, Naveed Ahmed; Al-Hazzani, Amal A.; Alsaif, Mohammed A.; Alsaif, Abdulaziz

doi:10.1371/journal.pone.0030049

Cited by 78 publications

(78 citation statements)

References 27 publications

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“…The results of this study are consistent with those of three previous studies, in which the rs3746444 GG genotype was shown to be associated with a decreased risk of cancer (12)(13)(14). By contrast, in six other studies (5,(15)(16)(17)(18)(19), the GG genotype was found to be associated with a higher risk of cancer development. There have also been studies that have found no association between this SNP and cancer risk (8,(20)(21)(22)(23)(24).…”

Section: Discussionsupporting

confidence: 91%

Noncoding RNA–related polymorphisms in pediatric acute lymphoblastic leukemia susceptibility

et al. 2014

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Background: Evidence for an inherited genetic risk for pediatric acute lymphoblastic leukemia has been provided in several studies. Most of them focused on coding regions. However, those regions represent only 1.5% of the entire genome. In acute lymphoblastic leukemia (ALL), it has been suggested that the expression of microRNAs (miRNAs) is dysregulated, which suggests that they may have a role in ALL risk. Changes in miRNA function may occur through single-nucleotide polymorphisms (SNPs). Therefore, the aim of this study was to evaluate whether polymorphisms in premiRNAs, and/or miRNA-processing genes, contribute to a predisposition for childhood ALL. Methods: In this study, we analyzed 118 SNPs in pre-miRNAs and miRNA-processing genes in 213 B-cell ALL patients and 387 controls. results: We found 11 SNPs significantly associated with ALL susceptibility. These included three SNPs present in miRNA genes (miR-612, miR-499, and miR-449b) and eight SNPs present in six miRNA biogenesis pathway genes (TNRC6B, DROSHA, DGCR8, EIF2C1, CNOT1, and CNOT6). Among the 118 SNPs analyzed, rs12803915 in mir-612 and rs3746444 in mir-499 exhibited a more significant association, with a P value <0.01. conclusion: The results of this study indicate that SNP rs12803915 located in pre-mir-612, and SNP rs3746444 located in pre-mir-499, may represent novel markers of B-cell ALL susceptibility. a cute lymphoblastic leukemia (ALL) is the most common pediatric hematological malignancy in developed countries. Its etiology is believed to be multifactorial, with both environmental and genetic risk factors being relevant (1). Recently, several studies have provided evidence for an inherited genetic risk for pediatric ALL (2,3). Most of these studies focused on the coding regions of these genetic components. However, this represents only ~1.5% of the entire genome, and noncoding regions of the genome have also been shown to mediate regulatory functions. For example, microRNAs (miRNAs) are a class of small noncoding RNA molecules that regulate gene expression at the posttranscriptional level by binding to the 3′ untranslated region of a target gene (4). This can lead to an inhibition of translation or enhanced degradation of a target mRNA (Figure 1). Primary double-stranded miRNA transcripts (pri-miRNA) are processed in the nucleus by microprocessor machinery, which includes DROSHA RNase and the double-stranded RNA-binding protein, DGCR8. A hairpin precursor miRNA molecule of 70-100 nucleotides (pre-miRNA) is then produced, and its translocation into the cytoplasm is facilitated by RAN GTPase and Exportin 5 (XPO5). In the cytoplasm, pre-miRNAs are further processed by a protein complex that includes DICER1, TRBP, EIF2C1, EIF2C2, GEMIN3, and GEMIN4, resulting in the production of mature miRNAs (4). It has been predicted that there are more than 1,000 miRNA genes in the human genome (5), and ~30% of human genes are regulated by miRNAs.In the past few years, it was suggested that miRNAs in ALL are dysregulated. For example, in the study of Zhang ...

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Section: Discussionsupporting

confidence: 91%

Noncoding RNA–related polymorphisms in pediatric acute lymphoblastic leukemia susceptibility

et al. 2014

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“…This C > G polymorphism results in a change from C: U pair to G: U mismatch in the stem structure of miR-146a precursor. Several studies have been investigated the association of miR-146a rs2910164 polymorphism and risk of various cancer (Jazdzewski et al, 2008;Hu et al, 2009;Catucci et al, 2010;Okubo et al, 2010;Zeng et al, 2010;Garcia et al, 2011;Alshatwi et al, 2012;Hezova et al, 2012;Min et al, 2012;Zhou et al, 2012;Chae et al, 2013;Hu et al, 2013;Lv et al, 2013;Ma et al, 2013;Marino et al, 2013;Wei et al, 2013;Vinci et al, 2013). Our results did not support a genetic association between miR-146a rs2910164 and susceptibility to CRC.…”

Section: Discussioncontrasting

confidence: 82%

Associations of Single Nucleotide Polymorphisms in miR-146a, miR-196a, miR-149 and miR-499 with Colorectal Cancer Susceptibility

Du¹,

Ma²,

Zhao³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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“…One study was excluded as it was not associated with hsa-mir-499 rs3746444 A>G (Kupcinskas et al, 2012). After further excluding two abstracts (Hu et al, 2009;Hwang et al, 2010), thirty-nine case-control studies involving 14,136 cases and 16,937 controls were selected for meta-analysis (Hu et al, 2009;Tian et al, 2009;Catucci et al, 2010;Liu et al, 2010;Okubo et al, 2010;Srivastava et al, 2010;Akkiz et al, 2011;George et al, 2011;Ling et al, 2011;Mittal et al, 2011;Vinci et al, 2011;Zhou et al, 2011;Alshatwi et al, 2012;Chu et al, 2012;Kim et al, 2012;Min et al, 2012;Xiang et al, 2012;Zhou et al, 2012;Ahn et al, 2013;Lv et al, 2013;Shan et al, 2013;Song et al, 2013;Umar et al, 2013;Vinci et al, 2013;Wei et al, 2013;Wu et al, 2013;Zou and Zhao, 2013;Bansal et al, 2014;Chu et al, 2014;Du et al, 2014;Gutierrez-Camino et al, 2014;Hasani et al, 2014;Hou et al, 2014;Hu et al, 2014;Ma et al, 2014;Omrani et al, 2014;Pu et ...…”

Section: Methodsmentioning

confidence: 99%

Lack of Association between Hsa-Mir-499 rs3746444 Polymorphism and Cancer Risk: Meta-analysis Findings

Jiang

Chen²,

Tang³

et al. 2015

Asian Pacific Journal of Cancer Prevention

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Epidemiologic findings concerning the association between the hsa-mir-499 rs3746444 A>G polymorphism and cancer risk have yielded mixed results. We aimed to investigate the association by performing a meta-analysis of all available studies. We searched PubMed and EMBASE for studies published up to November 2014, using odds ratios (ORs) with 95% confidence intervals (CIs) to assess the strength of any association. The BenjaminiHochberg (BH) method was used to correct the p values for multiple comparisons. We included 39 studies, including 14,136 cases and 16,937 controls. The results of overall meta-analysis suggested a borderline association between hsa-mir-499 rs3746444 polymorphism and cancer susceptibility (AG+GG vs. AA: OR=1.15, 95% CI= 1.04-1.26, corrected p value=0.04). After removing studies not conforming to Hardy-Weinberg equilibrium (HWE), however, this association disappeared (AG+GG vs AA: OR=1.18, 95% CI=1.03-1.34, corrected p value=0.21). When stratified analysis by ethnicity, cancer type or HWE in controls, although some associations between hsa-mir-499 rs3746444 polymorphism and cancer susceptibility were detected, these associations no longer existed after adjustment using BH method. In conclusion, our meta-analysis suggests that hsa-mir-499 rs3746444 A>G polymorphism is not associated with risk of cancer based on current evidence. conclusive. Many meta-analyses have examined the association of the polymorphism with cancer risk and the lasted one suggested that hsa-mir-499 rs3746444 polymorphism was a risk factor for cancer development. Since the latest meta-analysis had been performed, nineteen case-control studies (Ling

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Differential Expression Profile and Genetic Variants of MicroRNAs Sequences in Breast Cancer Patients

Cited by 78 publications

References 27 publications

Noncoding RNA–related polymorphisms in pediatric acute lymphoblastic leukemia susceptibility

Noncoding RNA–related polymorphisms in pediatric acute lymphoblastic leukemia susceptibility

Associations of Single Nucleotide Polymorphisms in miR-146a, miR-196a, miR-149 and miR-499 with Colorectal Cancer Susceptibility

Lack of Association between Hsa-Mir-499 rs3746444 Polymorphism and Cancer Risk: Meta-analysis Findings

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