Differential Expressions of microRNAs and Transfer RNA-derived Small RNAs: Potential Targets of Choroidal Neovascularization

Zhang, Liwei; Liu, Shaohua; Wang, Jiang-Hui; Zou, Jingling; Zeng, Huilan; Zhao, Han; Zhang, Boxiang; He, Yan; Shi, Jingming; Zhou, Yedi

doi:10.1080/02713683.2019.1625407

Cited by 24 publications

(22 citation statements)

References 43 publications

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“…Although increasing evidence shows that miRNAs and tsRNAs play crucial roles in choroidal neovascularization 29 , 34 , few studies investigated how does tsRNA involve in RNV pathogenesis. In this study, we carried out a small RNA sequencing of tRFs and tiRNAs to profile their expression in the retinas of OIR mice.…”

Section: Discussionmentioning

confidence: 99%

“…Pretreatment of tsRNA and library preparation, small RNA sequencing, collection and analysis of the data were conducted as described 29 . In brief, each RNA sample was sequentially ligated to 3' and 5' small RNA adapters.…”

Section: Methodsmentioning

confidence: 99%

“…qRT-PCR experiments were performed on ViiA 7 Real-time PCR System (Applied Biosystems, Foster City, CA, USA) by using 2 × PCR Master Mix (Arraystar). Reaction conditions were described in our previous study 29 . The 2-ΔΔCt method 30 was applied in the calculation of tsRNA expression levels normalized with U6.…”

Section: Methodsmentioning

confidence: 99%

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Small RNA Sequencing Reveals Transfer RNA-derived Small RNA Expression Profiles in Retinal Neovascularization

Peng¹,

Zou²,

Wang³

et al. 2020

Int. J. Med. Sci.

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Retinal neovascularization (RNV) is characterized in retinopathy of prematurity (ROP), diabetic retinopathy (DR), and retinal vein occlusion (RVO), which leads to severe vision loss and even blindness. To reveal the altered transfer RNA-derived small RNA (tsRNA)s in RNV, and to investigate the underlying mechanisms of the altered tsRNAs involved in RNV, we carried out a small RNA sequencing to profile tsRNA expressions in the retinas of mice with oxygen-induced retinopathy (OIR) and control mice. A total of 45 tsRNAs were significantly changed (fold change ≥ 1.5 and P < 0.05) in the retinas of OIR mice compared with controls. Validation by quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) in four selected tsRNAs was consistent with the results of small RNA sequencing. Bioinformatics analyses identified 153 altered target genes of the four validated tsRNAs. These altered target genes were largely enriched in developmental process, cell periphery and protein binding, as well as Th1 and Th2 cell differentiation pathway. Our study suggests tsRNAs play key roles in the pathogenesis of RNV, indicating their therapeutic potential to treat patients with RNV. Moreover, small RNA sequencing is a useful tool to identify changes in tsRNA expression, an important indicator of the progress of retinal diseases.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Small RNA Sequencing Reveals Transfer RNA-derived Small RNA Expression Profiles in Retinal Neovascularization

Peng¹,

Zou²,

Wang³

et al. 2020

Int. J. Med. Sci.

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“…As previously described, the CNV mouse model was induced by laser photocoagulation [27][28][29] with some modification. In brief, the photocoagulation was performed with 25 spots in each eye using a 532-nm diode laser (100 mW, 0.1 s duration, 50 μm).…”

Section: Laser-induced Cnv Mouse Modelmentioning

confidence: 99%

“…Previous studies using the CNV mouse model to examine the role of VEGF and many other molecules [25][26][27]. We recently identified altered microRNAs and tRNA-derived small RNAs in the laser-induced CNV model [28].…”

Section: Introductionmentioning

confidence: 99%

Altered Long Non-coding RNAs Involved in Immunological Regulation and Associated with Choroidal Neovascularization in Mice

et al. 2020

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Choroidal neovascularization (CNV) is a severe complication of the wet form of age-related macular degeneration (AMD). Long non-coding RNAs (lncRNAs) have been implicated in the pathogenesis of different ocular neovascular diseases. To identify the function and therapeutic potential of lncRNAs in CNV, we assessed lncRNAs and mRNA expression profile in a mouse model of laser-induced CNV by microarray analysis. The results of altered lncRNAs were validated by qRT-PCR. Bioinformatics analyses, including Gene Ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, were performed to clarify the potential biological functions and signaling pathways with which altered genes are most closely related. Moreover, to identify the interaction of lncRNAs and mRNAs, we constructed a coding-non-coding gene co-expression (CNC) network. By microarray analysis, we identified 716 altered lncRNAs and 821 altered mRNAs in CNV mice compared to controls. A CNC network profile based on 7 validated altered lncRNAs (uc009ewo.1, AK148935, uc029sdr.1, ENSMUST00000132340, AK030988, uc007mds.1, ENSMUST00000180519) as well as 282 interacted and altered mRNAs, and were connected by 713 edges. GO and KEGG analyses suggested that altered mRNAs, as well as those lncRNA-interacted mRNAs were enriched in immune system process and chemokine signaling pathway. Thus, lncRNAs are significantly altered in this mouse model of CNV and are involved in immunological regulation, suggesting that lncRNAs may play a critical role in the pathogenesis of CNV. Thus, dysregulated lncRNAs and their target genes might be promising therapeutic targets to suppress CNV in AMD.

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tRNA-Derived Small RNAs: Novel Insights into the Pathogenesis and Treatment of Cardiovascular Diseases

Wang

Luo

Yuan

et al. 2022

J. of Cardiovasc. Trans. Res.

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Differential Expressions of microRNAs and Transfer RNA-derived Small RNAs: Potential Targets of Choroidal Neovascularization

Cited by 24 publications

References 43 publications

Small RNA Sequencing Reveals Transfer RNA-derived Small RNA Expression Profiles in Retinal Neovascularization

Small RNA Sequencing Reveals Transfer RNA-derived Small RNA Expression Profiles in Retinal Neovascularization

Altered Long Non-coding RNAs Involved in Immunological Regulation and Associated with Choroidal Neovascularization in Mice

tRNA-Derived Small RNAs: Novel Insights into the Pathogenesis and Treatment of Cardiovascular Diseases

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