Differential fluorescent staining of Drosophila chromosomes with quinacrine mustard

Adkisson, Karen P.; Perreault, W. J.

doi:10.1007/bf00285186

Cited by 37 publications

(9 citation statements)

References 14 publications

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“…The observation of a quinacrine-bright area in D. virilis at the base of chromosome four is consistent with the results of ADKISSON et al ( 1971 ). However, ADKISSON and colleagues reported only a single band, and presumably did not observe the doublet.…”

Section: Discussionsupporting

confidence: 85%

“…In recent years, it has been demonstrated that quinacrine can be used to localize certain AT-rich satellite DNA sequences in metaphase chromoso· mes (PARDUE 1973;ADKISSON et al 1971;GALL et al 1971).…”

Section: Relationship Of Q-bands To Satellite Dnamentioning

confidence: 99%

“…Secondly, several members of this group are known to exhibit quinacrinebright bands (ADKISSON et al 1971;BARR and ELLISON 1971a;HoLM-QUIST 1975, 1976SINIBALDI et al 1976). Several labs have studied the satellite DNA isolated from some of the taxa (GALL et al 1971;BLUMENTHAL 1973;SCHWEBER 1974) and 5S ribosomal genes have been localized in a few members of the group (CoHEN 1976).…”

Section: Introductionmentioning

confidence: 99%

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Distribution and Evolution of Q-Bands in the Polytene Chromosomes of the Virilis Group

Sinibaldi

Barr

1979

Caryologia

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Section: Discussionsupporting

confidence: 85%

Section: Relationship Of Q-bands To Satellite Dnamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Distribution and Evolution of Q-Bands in the Polytene Chromosomes of the Virilis Group

Sinibaldi

Barr

1979

Caryologia

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“…It has been shown that quinacrine renders some heterochromatic regions brightly fluorescent (Caspersson et al 1969;Adkisson et al 1971). More recently, Ellison and Barr (1972) demonstrated bright quinacrine fluorescence in Samoaia leonensis chromosomes in areas of adenineand thymine-rich DNA.…”

Section: Discussionmentioning

confidence: 99%

Polytene chromosomes of three species of blackflies in the Simulium pictipes group (Diptera: Simuliidae)

Bedo¹

1975

Can. J. Zool.

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BEDO, D. G. 1975. Polytene chromosomes of three species of blackflies in the Simuliumpictipes group (Diptera: Simuliidae). Can. J. Zool. 53: 1147-1 164. The salivary gland chromosomes of members of the Simulium pictipes group were examined by conventional staining and quinacrine fluorescence staining methods which proved to be a useful tool to show otherwise hidden details, especially in expanded centromere regions. Three cytologically definable species were found, one of which must represent a previously unrecognized species. Simulium longistylatum Shewell was the only species among the three siblings identified with certainty. The remaining pair were designated as S. pictipes A and S. pictipes B. In all three siblings the haploid chromosome number was three. Specific differences include a simple and a complex inversion, a shift of basal bands between the short arms of the second and third chromosome, details of the sex chromosomes, and the amount of DNA in certain individual bands and expanded centromere regions. Y chromosome markers are located in a different element of the complement in each of the three species, a unique situation within closely related blackfly sibling species. These findings are discussed in connection with the evolution of sex chromosomes and sibling species. A cytophylogeny is presented.BEDO, D. G. 1975. Polytene chromosomes of three species of blackflies in the Simulium pictipes group (Diptera: Sirnuliidae). Can. J. Zool. 53: 1 147-1 164. On a examine les chromosomes des glandes salivaires chez des individus du groupe Simulium pictipes par des mtthodes classiques de coloration et des methodes de coloration fluorescente a la quinacrine; ces methodes mettent en evidence certains details ordinairement invisibles, particulierement dans les rkgions renflees du centromere. On reconnait cytologiquement trois especes dont I'une est tres certainement une esptce inconnue jusqu'ici. Des trois especes jumelles, Simulium longistylatum est la seule a pouvoir btre identifiee avec certitude. On dCsigne les deux autres especes par les termes S . pictipes A et S . pictipes B. Chez les trois especes, le nombre chromosomique haploide est de trois. Parmi les differences spkcifiques, on compte une inversion simple et une inversion complexe, un transfert des stries basales entre les portions courtes du second et du troisieme chromosomes; il y a egalement de ltgtres differences entre les chromosomes sexuels et enfin certaines stries individuelles de mbme que les renflements du centromere contiennent des quantites differentes d'ADN. On a decouvert aussi un caractere differentiel unique entre des especes tres parentes de mouches noires jumelles: les marqueurs du chromosome Y sont situks dans un Clement different du complement chez chacune des trois espkces. La discussion porte sur I'Cvolution des chromosomes sexuels et des especes jumelles.

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“…melanogaster (Rodriguez-Alfageme et al, 1976). This study noted a correlation between the chromosomal sites that fluoresce most intensely with anti-Dl anti bodies and the sites known to fluoresce most intensely with quinacrine (Vosa, I97O;Adkisson et al, 1971;Ellison and Barr, 1972;Mayfield and Ellison, 1975;Hochman, 1976). A more carefully controlled follow-up investigation revealed that, under conditions that minimize nonspecific antibody interactions, D1 is widely distributed throughout the cytoplasm and nucleus, present in many chromomeras, but most abundant in chromosomal sites that contain the A-T rich satellite DNA of buoyant density 1.672 g ml ^ (Rodriguez-Alfageme et al, 1980).…”

Section: Protein D1mentioning

confidence: 90%

Chromosomal distribution of Drosophila HMG-like proteins

Bassuk

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Fractionation of nucleosomes in preparative agarose gels Method 1 Method 2 Comments on the two methods Analytical agarose gel electrophoresis Polyacrylamide gel electrophoresis Triton/acid/urea first dimension tube gels 30 Acid-soluble nuclear protein samples 31 Agarose fractionated nucleosomal samples 31 Triton/acid/urea slab gels SDS slab gels Staining of proteins Coomassie Brilliant Blue R staining SiIver staining 32 DNA electrophoresis Photography Determination of Materials 34 Acrylamide Other reagents RESULTS I. ELECTROPHORESIS OF ACID-SOLUBLE NUCLEAR PROTEINS RESULTS II. PURIFICATION OF PUTATIVE HMG PROTEINS RESULTS III. IMMUNOFLUORESCENCE STUDIES OF A13 RESULTS IV.

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Differential fluorescent staining of Drosophila chromosomes with quinacrine mustard

Cited by 37 publications

References 14 publications

Distribution and Evolution of Q-Bands in the Polytene Chromosomes of the Virilis Group

Distribution and Evolution of Q-Bands in the Polytene Chromosomes of the Virilis Group

Polytene chromosomes of three species of blackflies in the Simulium pictipes group (Diptera: Simuliidae)

Chromosomal distribution of Drosophila HMG-like proteins

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