Differential gene expression analysis of human cumulus cells

Abstract

Objective This study was performed to explore the possibility that each oocyte and its surrounding cumulus cells might have different genetic expression patterns that could affect human reproduction. Methods Differential gene expression analysis was performed for 10 clusters of cumulus cells obtained from 10 cumulus-oocyte complexes from 10 patients. Same procedures related to oocyte maturation, microinjection, and microarray analyses were performed for each group of cumulus cells. Two differential gene expression analyses were performed: one for the outcome of clinical pregnancy and one for the outcome of live birth. Results Significant genes resulting from these analyses were selected and the top 20 affected pathways in each group were analyzed. Circadian entrainment is determined to be the most affected pathway for clinical pregnancy, and proteoglycans in cancer pathway is the most affected pathway for live birth. Circadian entrainment is also amongst the 12 pathways that are found to be in top 20 affected pathways for both outcomes, and has both lowest p -value and highest number of times found count. Conclusion Although further confirmatory studies are necessary, findings of this study suggest that these pathways, especially circadian entrainment in cumulus cells, may be essential for embryo development and pregnancy.

Evaluation the Protective Effect of Withania somnifera Extract on the Level of Sex Hormone in Morphine Addicted Female Rats

“…The findings of the present research suggested that the W. somnifera has protective effect against morphine on the regulation of hormone as proved in Tables 1 and 2 which illustrated that promotive and protective effect of W. somnifera against noxious effect of the morphine, the result approved and assert by the previous studies, which Karimi et al, 2018 [26] showed that administration of morphine leads to irregulating and disturbance on the hypothalamus pituitary ovary axis and causes anovulatory infertility [32]. Scientists suggested that mechanism effect of morphine is related to the nature of the opioid receptors pharmacokinetic [26]; which morphine has inflammatory effects on the ovary due to causes increase in the secretion of LH in response to GnRH [33], and by inhibit effect of insulin in liver which that stimulate the pancreas for production of insulin [34], then, causes hyperinsulinemia in the polycystic ovary.…”

The Function of Cumulus Cells in Oocyte Growth and Maturation and in Subsequent Ovulation and Fertilization

“…CCs are the somatic cells surrounding the oocyte. They play an important role in the growth of the oocyte, meiotic maturation, ovulation, and fertilization in mammalians [ 4 ]. CCs originate from undifferentiated granulosa cells (GCs) from primordial to preantral follicles.…”

Whole-Genome Sequencing Reveals the Genomic Characteristics and Selection Signatures of Hainan Black Goat

“…Differential gene expression analysis of the 10 clusters of cumulus cells obtained from 10 cumulus–oocyte complexes from 10 patients was performed. Circadian entrainment is the most affected pathway in clinical pregnancy [ 35 ], which indirectly indicates that circadian entrainment affects the reproductive cycle. According to previous studies, the Rap-1 signaling pathway has an impact on reproduction [ 36 , 37 ].…”