Differential gene expression in mesothelioma

Rihn, Bertrand; Mohr, Steve; McDowell, Susan A.; Binet, Stéphane; Loubinoux, Julien; Galateau, F; Keith, Gérard; Leikauf, George D.

doi:10.1016/s0014-5793(00)01913-x

Cited by 61 publications

(32 citation statements)

References 19 publications

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“…Consistent with results reported in other studies [15]as well as in prior work [16], not all of the differences found by the array were reproducible by RT-PCR. Therefore, data obtained by array analysis require confirmation by other methods, like real-time RT-PCR or Northern blotting with K [17], kinetic real-time RT-PCR allowing the most exact quantification of transcription state comparison efficacy with an accuracy of ±20% [18]. …”

Section: Discussionmentioning

confidence: 99%

Characterization of Differential Gene Expression in Quiescent and Invasive Human Arterial Smooth Muscle Cells

Blindt¹,

Vogt²,

Lamby³

et al. 2002

J Vasc Res

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Proliferation, migration and invasion of smooth muscle cells (SMCs) are essential pathogenic processes in the development of a broad spectrum of cardiovascular disorders, like arteriosclerosis, restenosis after percutaneous transluminal angioplasty and stent implantation as well as transplant vessel disease. As an in vitro model mimicking these processes, the Boyden chamber was employed to characterize the diverging migratory and invasive potentials of proliferating and nonproliferating human arterial SMCs (haSMCs). Using this model, differential gene expression of both phenotypes was analyzed by a cDNA array system (Clontech human cardiovascular array). With these arrays, 558 cardiovascular-associated genes could be compared. Further, gene expression was exactly quantified by real-time RT-PCR. Protein expression was analyzed by ELISA and Western blotting. In total, 47 genes were differentially expressed more than 1.5 times. Most of the differentially regulated genes in this study were associated with the extracellular matrix (ECM) and cell motility. In detail, the respective groups were matrix-organizing proteins, ECM proteins, cell adhesion proteins, extracellular communication and cytoskeleton motility proteins. Genes known to be differentially regulated during haSMC migration and invasion, like TIMP 2, TIMP 3, and MMP 3, were confirmed by the array data. Reduced expression of several cytoskeletal proteins, like vimentin, fibronectin, cytokeratins and β1 integrin, was shown in the invasive phenotype. Further, angio-associated protein, alpha E-catenin and atrial brain natriuretic peptide receptor were downregulated whereas TFPI 2 was strongly upregulated in invasive haSMCs. In conclusion, several relevant potential candidate genes for the quiescent and the invasive SMC phenotype were identified and genes already known to be differentially regulated by previous analysis were confirmed.

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Section: Discussionmentioning

confidence: 99%

Characterization of Differential Gene Expression in Quiescent and Invasive Human Arterial Smooth Muscle Cells

Blindt¹,

Vogt²,

Lamby³

et al. 2002

J Vasc Res

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“…A mesothelioma cell line, MSTO-211H, was recently shown to express large amounts of Dio2 mRNA by microarray analysis (accession number U53506), offering a potential system to resolve the issue of D2 identity (23).…”

mentioning

confidence: 99%

The Human Type 2 Iodothyronine Deiodinase Is a Selenoprotein Highly Expressed in a Mesothelioma Cell Line

Curcio

Baqui

Salvatore

et al. 2001

Journal of Biological Chemistry

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Se-p31 ϳ2-3-fold. MG132 or lactacystin (10 M), inhibitors of the proteasome pathway by which D2 is degraded, increase both D2 activity and 75 Se-p31 3-4-fold and prevent the loss of D2 activity during cycloheximide or substrate (T 4 ) exposure. Immunocytochemical studies with affinitypurified anti-hD2 antibody show a Se-dependent increase in immunofluorescence. Thus, human D2 is encoded by hDio2 and is a member of the selenodeiodinase family accounting for its highly catalytic efficiency in T 4 activation.

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“…In recent years, a number of aberrantly expressed genes were suggested, but with a poor consistency among studies. In 2012, in order to find a consensus among nine independent transcriptome studies carried out between 2000 and 2010 (1)(2)(3)(4)(5)(6)(7)(8)(9), it has been performed a review and a data mining (10) and it has been identified a group of 96 overexpressed genes in MPM compared to non-malignant tissues and cell lines. This group of genes have been further evaluated in 2015 through a gene expression study performed on an independent cohort of mesothelioma tissues compared to non-malignant tissues: the overexpression of 51 genes ( Table 1) have been confirmed (11).…”

Section: Overexpressed Genes Identified In Malignant Pleural Mesothelmentioning

confidence: 99%

Overexpressed genes in malignant pleural mesothelioma: implications in clinical management

2018

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Malignant pleural mesothelioma (MPM) is a very aggressive cancer poorly responsive to current therapies. MPM patients have a very poor prognosis with a median survival of less than one year from the onset of symptoms. The biomarkers proposed so far do not lead to a sufficiently early diagnosis for a radical treatment of the disease. Thus, the finding of novel diagnostic and prognostic biomarkers and therapeutic targets is needed. Gene overexpression has been frequently associated with a malignant phenotype in several cancer types; therefore the identification of overexpressed genes may lead to the detection of novel prognostic or diagnostic marker and to the development of novel therapeutic approaches, based on their inhibition. In the last years, several overexpressed genes have been identified in MPM through gene expression profiling techniques: among them it has been found a group of 51 genes that resulted overexpressed in more than one independent study, revealing their consistency among studies. This article reviews the clinical implications of confirmed overexpressed genes in MPM described so far in literature.

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Differential gene expression in mesothelioma

Cited by 61 publications

References 19 publications

Characterization of Differential Gene Expression in Quiescent and Invasive Human Arterial Smooth Muscle Cells

Characterization of Differential Gene Expression in Quiescent and Invasive Human Arterial Smooth Muscle Cells

The Human Type 2 Iodothyronine Deiodinase Is a Selenoprotein Highly Expressed in a Mesothelioma Cell Line

Overexpressed genes in malignant pleural mesothelioma: implications in clinical management

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