Differential gene expression profile of MAGE family in taiwanese patients with colorectal cancer

Chung, Fu-Yen; Cheng, Tian−Lu; Chang, Hao‐Ming; Chiu, Hua-Hsien; Huang, Ming-Yii; Chang, Ming-Sung; Chen, Chung-Chi; Yang, Mei‐Sang; Wang, Jaw‐Yuan; Lin, Shiu‐Ru

doi:10.1002/jso.21580

Cited by 18 publications

(21 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Melanoma-associated antigens (MAGE) are a group of well-characterized members of the cancer/testis antigen (CTA) family, which represents a unique class of tumor antigens that are expressed in a wide variety of malignant tumors 7 8 . MAGE-A is a subfamily comprising MAGE and MAGE-A antigens that are highly tumor specific; MAGE-A genes are anomalously expressed cancers though rarely expressed in normal tissues 9 .…”

mentioning

confidence: 99%

High expression of MAGE-A9 correlates with unfavorable survival in hepatocellular carcinoma

Zhang

et al. 2014

Sci Rep

View full text Add to dashboard Cite

Melanoma-associated antigens (MAGE)-A9 has been reported to play important roles in the development of human cancers. However, the association between MAGE-A9 expression and the clinicopathological characteristics of hepatocellular carcinoma (HCC) is not well understood. The study was to detect the expression of MAGE-A9 in human HCC and investigate the association between its expression and the clinicopathological characteristics of HCC. Reverse transcription-polymerase chain reaction (RT-PCR), one-step quantitative -PCR (qPCR) and immunohistochemistry (IHC) analyses were performed to characterize the expression of MAGE-A9 in HCC cell lines and tissues. Kaplan-Meier survival and Cox regression analyses were employed to evaluate the prognosis of 100 HCC patients. The results showed that the expression of MAGE-A9 in HCC was significantly higher than that in non-cancerous cells and tissues. Moreover, the expression level of the MAGE-A9 protein in HCC was related to the pathological grade (p = 0.003), portal vein invasion (p = 0.001), distant metastasis (p = 0.022) and TNM stage (p = 0.005). Cox regression analysis further revealed that MAGE-A9 expression is an independent prognostic factor for disease-free survival (p = 0.006) and overall survival (p = 0.022). These data are the first to indicate that MAGE-A9 expression is a valuable prognostic biomarker for HCC and that high MAGE-A9 expression suggests unfavorable survival outcomes in HCC patients.

show abstract

mentioning

confidence: 99%

High expression of MAGE-A9 correlates with unfavorable survival in hepatocellular carcinoma

Zhang

et al. 2014

Sci Rep

View full text Add to dashboard Cite

show abstract

“…MAGED1 expression was also evaluated by Chung et al in Taiwanese CRC patients, and reported MAGED1 overexpression occurred in 45% CRC patients [15]. In the present study, 131 CRC patients were enrolled to compare their MAGED1 expression between colorectal cancer tissues and paired adjacent non-tumorous tissues.…”

Section: Discussionmentioning

confidence: 84%

“…Different from the MAGED1, MAGED12 was reported frequently up-regulated in tumors [15,17]. It was reported that MAGED1 and -D2 RNA had different distribution during the embryonic development and brain development [18].…”

Section: Discussionmentioning

confidence: 99%

“…It was reported that the expression of MAGED1 was down-regulated in breast carcinoma cell lines [13] and in glioma stem cells [14]. Chung et al examined the expression profile of MAGE family genes in Taiwanese patients with colorectal cancer and discovered that the type II MAGE genes MAGED12 , MAGEF1 , and MAGEH1 are frequently up-regulated in tumors [15]. …”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Prognostic relevance of melanoma antigen D1 expression in colorectal carcinoma

Zeng

Yang

et al. 2012

J Transl Med

View full text Add to dashboard Cite

BackgroundMelanoma antigen D1 (MAGED1) is a member of the type II melanoma antigen (MAGE) family. The down-regulation of MAGED1 expression has been shown in breast carcinoma cell lines and in glioma stem cells and may play an important role in apoptosis and anti-tumorigenesis. However, there is no report on its clinical role in colorectal cancer (CRC).MethodsWe examined the expression of MAGED1 by qPCR in colorectal cancer tissues and their adjacent non-tumorous tissues taken from 6 cases and performed Western blotting and IHC analyses. In addition, we analyzed MAGED1 expression in 285 clinicopathologically characterized colorectal cancer patients.ResultsMAGED1 expression was significantly down-regulated in colorectal cancer tissues compared with adjacent non-tumorous tissues and was associated with clinical stage (p < 0.001), T classification (p = 0.001), N classification (p < 0.001), M classification (p < 0.001) and pathologic differentiation (p = 0.002). Patients with lower MAGED1 expression had a shorter survival time than those with higher MAGED1 expression. Univariate and multivariate analyses indicated that MAGED1 expression was an independent prognostic factors (p < 0.001).ConclusionsMAGED1 may serve as a novel prognostic biomarker of human colorectal cancer.

show abstract

“…The procedure of the membrane array was performed based on our previously described protocol. 9 Subsequent quantification analysis of each spot's intensity was carried out using AlphaEase FC software (Alpha Innotech Corp., San Leandro, CA USA). The spots that consistently carried a factor of 2 were considered to be differentially expressed.…”

Section: Methodsmentioning

confidence: 99%

The use of multiple molecular markers as predictors of the clinical prognosis of patients with colorectal cancer

Tung¹,

Lin²,

Wang³

et al. 2012

Genomic Medicine, Biomarkers, and Health Sciences

Self Cite

View full text Add to dashboard Cite

Serum carcinoembryonic antigen (CEA) is most commonly used as a prognostic biomarker for evaluating curatively resected colorectal cancer (CRC) patients, but it has a low sensitivity and specificity. The aim of this study was to evaluate potential genetic markers in CRC patients using membrane array. Fifty CRC patients were enrolled and mRNA expression in their tissues were analyzed using membrane array analysis. Seven genes were analyzed in this study, including ATP2A2, GLUT1, MMP13, MAGE-A2, MAGE-A7, MAGE-A8, and MAGE-A12. Correlations between the results of the membrane array and the clinicopathological features of these CRC patients were then evaluated. The results show that the overexpression of any three or four of these seven genes is correlated with tumor invasion depth, lymphatic invasion, advanced stage, and postoperative recurrence (all p < 0.005). Furthermore, the expression of any four genes was more significantly correlated with clinicopathological characteristics than the expression of only two or three genes. The combination of multiple molecular markers and the membrane array method might be useful for predicting postoperative relapse in CRC patients.

show abstract

Differential gene expression profile of MAGE family in taiwanese patients with colorectal cancer

Abstract: This is the first comprehensive report to clarify the differential expression profile of whole MAGE family in CRCs, and it might provide some crucial information about the carcinogenesis and progression in Taiwanese patients with CRC.

Cited by 18 publications

References 21 publications

High expression of MAGE-A9 correlates with unfavorable survival in hepatocellular carcinoma

High expression of MAGE-A9 correlates with unfavorable survival in hepatocellular carcinoma

Prognostic relevance of melanoma antigen D1 expression in colorectal carcinoma

The use of multiple molecular markers as predictors of the clinical prognosis of patients with colorectal cancer

Contact Info

Product

Resources

About