Differential gene expression profiles in human THP-1 monocytes treated with Lactobacillus plantarum or Staphylococcus aureus lipoteichoic acid

Abstract

The lipoteichoic acid (LTA) of Staphylococcus aureus (aLTA) and Lactobacillus plantarum (pLTA) engage the same toll-like receptor 2 (TLR2) signaling pathway but exert different effects on innate immunity and inflammation. The mechanisms underlying these differential effects are not yet clear. Human oligonucleotide microarrays were used to investigate the transcriptome of human THP-1 monocytes upon exposure to aLTA or pLTA, and differential gene expression profiles were observed between the aLTA-and pLTA-treated cells. The expression level of 1,302 genes in aLTAtreated cells increased more than 2-fold; some of which have been implicated in immune or inflammatory responses, cell adhesion, cell signal transduction, transcription factors, anion transport, proteolysis, and oxidative processes. Particularly, a variety of genes that encode cytokines and chemokines, and TLR signaling-related molecules belonging to the tumor necrosis factor receptor-associated factor (TRAF), nuclear factor-kappa B, and signal transducer and activator of transcription families were remarkably up-regulated by aLTA stimulation. In contrast, pLTA treatment altered the expression of only 90 genes by more than 1.5-fold, and these genes were not correlated with innate immunity, inflammation or other related processes. The different effects mediated by aLTA and pLTA were further verified and compared by analysis of the expression of a selected group of genes, including TRAFs and some cytokines and chemokines, using real time-polymerase chain reaction and ELISA. These data suggest that aLTA and pLTA have different immunomodulatory potentials. Compared with pLTA, aLTA is a stronger stimulator and impacts the expression of many innate immunity-and/or inflammation-related genes.

Lipoteichoic Acid Isolated from Staphylococcus aureus Induced THP-1 Cell Apoptosis through an Autocrine Mechanism of Cytokines and SOCS-1-Mediated Bcl2 Inhibition

“…In a previous study, we found that aLTA significantly induced manganese superoxide dismutase (MnSOD) production by 28.3-fold in THP-1 cells [11]. aLTA did not affect THP-1 viability in low dosages.…”

Next-generation sequencing predicts interaction network between miRNA and target genes in lipoteichoic acid-stimulated human neutrophils

“…Previous publications have reported that stimulation with 0.1-10 µ g/ml of S. aureus LTA induced the release of cytokines, such as IL-8 and TNF-α, in human monocytes within 1-6 h (38,39). In addition, the gene expression profile of the human monocyte cell line THP-1 following stimulation with 25 µ g/ml LTA for 6 h was detected via microarray analysis (40). The results indicated that genes involved in inflammatory responses, cell adhesion, cytokines and chemokines were upregulated following S. aureus LTA stimulation (40).…”

“…In addition, the gene expression profile of the human monocyte cell line THP-1 following stimulation with 25 µ g/ml LTA for 6 h was detected via microarray analysis (40). The results indicated that genes involved in inflammatory responses, cell adhesion, cytokines and chemokines were upregulated following S. aureus LTA stimulation (40). In the present study, to investigate the mRNA and miRNA expression changes in human neutrophils, human CD66abce + cells were enriched from peripheral blood obtained from a healthy donor.…”