Differential gene expression profiles in foetal skin of Rex rabbits with different wool density

Liu, Lihui; Li, B.; Zhu, Yanli; Wang, C.Y.; Li, F. C.

doi:10.4995/wrs.2016.2335

Cited by 4 publications

(3 citation statements)

References 26 publications

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“…DKK1 can inhibit the Wnt signaling pathway by inhibiting β-catenin activity, leading to hair follicle degeneration [29]. The hair follicle development of Rex rabbit mainly concentrated in 20-26 gestational age, the most active development was at 23-24 gestational age, and the basic development was formed at 25-26 gestational age [30]. Activation of the Wnt/β-catenin signaling pathway is a critical initial step in hair basal plate formation during hair follicle development in the embryonic period [31,32].…”

Section: Discussionmentioning

confidence: 99%

The Role of Wnt10b/β-Catenin Signaling Pathway in Hair Follicle Development in Different Ages of Rex Rabbits

Liu,

Zhang,

Bai

et al. 2024

Preprint

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In the present study, we aimed to observed hair follicle development and describe Wnt10b/β-catenin signaling pathways related to hair follicle development in different ages of Rex rabbits. Six Rex rabbits (3 male and 3 female) of 15, 30, 60, 90, 120 and 150 days of age were selected and skin samples were collected for hair follicle development and Wnt/β-catenin signaling pathway genes expresion detection. The results showed that the the values for body weight, coat length, skin thickness, skin area, and skin weight of Rex rabbits were increased significantly with age (p &lt; 0.05). The total hair follicle density, secondary hair follicle density and the ratio of the secondary hair follicle density to the primary hair follicle density in 120 days of Rex rabbits was higher than that in the other ages (p &lt; 0.05), and the major hair follicles were in the growing period at 15 days and 30 days of age, some of them had already entered the degeneration period at 60 days of age, and most of them entered the degeneration period at 90 days of age, the presence of quiescent hair follicles and the enlargement of primary hair follicles were also observed. At 120 and 150 days of age, the back hair follicles entered the growing period, and the secondary hair follicles had obvious differentiation. Furthermore, the expression of the Wnt10b, CTNNB1, adenomatosis polyposis protein (APC), Disheveled 2 (DVL2), glycogen synthase kinase 3β（GSK-3β）, Lymphoid enhancer-binding factor 1 (LEF1), dickkopf-1 (DKK1) and transforming growth factor beta 1 (TGFβ-1) genes in the skin tissue of Rex rabbits at different age was significantly differences (p &lt; 0.05), and the expression of the Wnt10b, CTNNB1, DVL2, GSK-3β, and TGFβ-1 genes at 90 days was higher than that in the other days, the expression of the LEF1 and DKK1 genes at 60 days was higher than that in the other days. As western blotting indicated, the β-catenin protein phosphorylation (P-CTNNB1) levels in the skin tissue of Rex rabbits shows the trend of first increasing, then decreasing and then increasing, 60 days, 120 days and 150 days significantly higher than 15 days, 30 days or 90 days (p &lt; 0.05). With the increasing of ages, the GSK-3β protein phosphorylation (P-GSK-3β) levels in the skin tissue of Rex rabbits were increasing, and maximum at 120 days of age (p &lt; 0.05). These results show that ages could influence on the fur quality and hair follicle traits of Rex rabbits, and Wnt10b/β-catenin signaling pathways is involved in the biological process of periodic hair follicle development in Rex rabbits. Considering the rule of fur growth, the slaughter age of Rex rabbits should not be earlier than 120 days.

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Section: Discussionmentioning

confidence: 99%

The Role of Wnt10b/β-Catenin Signaling Pathway in Hair Follicle Development in Different Ages of Rex Rabbits

Liu,

Zhang,

Bai

et al. 2024

Preprint

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“…Met can be metabolized into cysteine and cystine to provide an adequate substrate for keratin protein formation and affect hair growth. In the previous study, KAP3.1 and 6.1 gene expression was higher in rabbits with high wool density than in rabbits with low wool density [ 18 ]. LP treatment reduced the expression of KAP3.1 and KAP6.1 , and the addition of Met significantly increased the expression of KAP3.1 and KAP6.1 , indicating that KAP3.1 and KAP6.1 are the key targets involved in Met promoting hair growth in LP-fed rabbits.…”

Section: Discussionmentioning

confidence: 99%

Dietary methionine supplementation to a low-protein diet improved hair follicle development of Angora rabbits

Zhao¹,

Wang²,

Wang³

et al. 2023

Anim Biosci

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Angora rabbits fed a low-protein diet exhibit decreased hair production performance. This study set out to evaluate the effects of methionine on hair properties and nitrogen metabolism in Angora rabbits fed a low-protein diet and investigated the gene expression related to hair follicle development to determine the possible molecular mechanism of methionine effects on hair follicle development. MethodsAn experiment was conducted to investigate the effects of DL-methionine addition on a low-protein diet on hair development in Angora rabbits. Angora rabbits were divided into 5 groups: fed a normal diet (control), fed a low-protein diet (LP), or fed an LP supplemented with 0.2%, 0.4%, or 0.6% DL-methionine (Met). ResultsThe results showed that rabbits in the LP group had lower wool yield than the control rabbits, but the addition of 0.4%-0.6% Met to LP attenuated these effects (P<0.05).

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“…Another gene was EPHA6 on chr6: 45,162,190–45,182,915 bp in pastel group. Down-regulation of EPHA6 expression was associated with low wool density in rabbit [ 71 ]. EPHA3 gene is a member of ephrins which was suggested to act as a hair development promoter [ 72 ] and had a potential role in the wool structure of sheep [ 73 ].…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Detection of Selection Signatures for Pelt Quality Traits and Coat Color Using Whole-Genome Sequencing Data in American Mink

Valipour

Karimi

Ngoc

et al. 2022

Genes

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Domestication and selection are the major driving forces responsible for creating genetic variability in farmed species. American mink has been under selection for more than 100 years for improved body size and pelt quality. This study aimed to identify the genomic regions subjected to selection for pelt quality traits, and coat color using the whole genome sequences of 100 mink raised in the Canadian Centre for Fur Animal Research (CCFAR) at Dalhousie Agriculture Campus (Truro, NS, Canada), and Millbank fur farm (Rockwood, ON, Canada). Measurements of three dried pelt characteristics (including pelt size (n = 35), overall quality of fur (n = 27), and nap size (n = 29)), and three coat color of Black, Stardust, and Pastel (Stardust_ Black (n = 38), and Pastel_Black (n = 41)) were used to assign animals to pairwise groups. Signatures of selection were detected using integrated measurement of fixation index (Fst), extended haplotype homozygosity (XP-EHH), and nucleotide diversity (θπ) tests. In total, overlapping top 1% of Fst and XP-EHH harbored 376 genes for pelt quality traits (110 for nap size, 163 for overall quality of fur, and 98 pelt size), and 194 genes for coat color (123 for Pastel_Black and 71 for Stardust_Black) were detected in different groups. Integrating results of Fst, and XP-EHH with the θπ test supported 19 strongly selected regions on chromosomes 3, 4, 5, 6, 7, 8, 9, and 10 that contained 33 candidate genes related to fur quality, hair follicle function, and pelt size traits. Gene ontology revealed numerous genes related to the hair cycle process and molting cycle process, epidermis development, Wnt signaling pathway and muscle development. This study provided the first map of putative selection signals related to pelt quality and coat color in American mink, which could be used as a reference for future studies attempting to identify genes associated with economically important traits in mink.

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Differential gene expression profiles in foetal skin of Rex rabbits with different wool density

Cited by 4 publications

References 26 publications

The Role of Wnt10b/β-Catenin Signaling Pathway in Hair Follicle Development in Different Ages of Rex Rabbits

The Role of Wnt10b/β-Catenin Signaling Pathway in Hair Follicle Development in Different Ages of Rex Rabbits

Dietary methionine supplementation to a low-protein diet improved hair follicle development of Angora rabbits

Genome-Wide Detection of Selection Signatures for Pelt Quality Traits and Coat Color Using Whole-Genome Sequencing Data in American Mink

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