Differential growth rates in stromal cultures of human prostate derived from patients of varying ages

Sensibar, Julia A.; Pruden, Shaina; Kasjanski, Richard; Rademaker, Alfred; Lee, Chung; Grayhack, John T.; Kozlowski, James M.

doi:10.1002/(sici)1097-0045(19990201)38:2<110::aid-pros4>3.0.co;2-r

Cited by 17 publications

(11 citation statements)

References 19 publications

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“…Similar alterations occurred in the adult gerbil ventral prostate when treated with finasteride, with a spreader arrangement of the smooth muscle and the wavy or sinuous collagen fibers (Corradi et al,2004). In human stromal culture cells, a differential growth rate and cellular shape changes occur with increasing donor age and/or the development of benign prostatic hyperplasia (BPH), indicating that there may be an inherent difference in prostate stromal composition with age (Sensibar et al,1999). Alterations in the form, amount, and distribution of prostate tissue elements seem to be common during aging in different species of mammals, including the gerbil.…”

Section: Discussionmentioning

confidence: 99%

Biological behavior of the gerbil ventral prostate in three phases of postnatal development

et al. 2006

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In this study, we characterized the gerbil's ventral prostate histology ultrastructurally and quantitatively throughout three phases of postnatal development (young, adult, and old) in order to comprehend its biological behavior and propensity to developing spontaneous lesions with aging. The gerbil prostate is composed of alveoli and ducts immersed in a stroma composed of smooth muscle, fibroblasts, collagen and elastic fibers and vessels. The prostate tissue components present morphological and quantitative aspects that vary according to age. Young animals have an immature gland with modest secretory activity. Synthetic activity remained stable in adult and old gerbil. However, prostatic morphology was altered in the aging, showing an increased epithelium and stromal fibrosis. The nuclei of the secretory cells increased with aging, whereas nucleoli presented few alterations during postnatal development. The epithelial proliferation and stromal remodeling noted in this study indicate that the gerbil prostate may respond to the androgen declines typical of senescence through epithelial proliferation and stromal remodeling. Anat Rec Part A, 288A: 723-733, 2006.

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Section: Discussionmentioning

confidence: 99%

Biological behavior of the gerbil ventral prostate in three phases of postnatal development

et al. 2006

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“…These findings seem to confirm the hypothesis that the increased Vv of smooth muscle fibres in BPH arises by cellular proliferation. In cellular cultures of stroma the proportion of smooth muscle cells is greater in BPH than in normal prostate [19]; this may be clinically important.…”

Section: Discussionmentioning

confidence: 99%

Stromal and acinar components of the transition zone in normal and hyperplastic human prostate

et al. 2002

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“…Most current work includes an analysis of smooth muscle markers such as ␣-smooth muscle actin, smooth muscle myosin, and/or desmin. The percentage of cells in the culture expressing smooth muscle markers varies with conditions, number of passages, and possibly age of the donor [12,28,29] or histology of the tissue of origin [6].…”

Section: Cell-type Markersmentioning

confidence: 99%

Cultured stromal cells: An in vitro model of prostatic mesenchymal biology

Peehl¹,

Sellers²

2000

Prostate

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BACKGROUND Initial efforts to develop in vitro models to study prostatic biology focused on the culture and characterization of epithelial cells. Recently, attention has turned towards inclusion of stromal cells in experimental systems. METHODS Improved methods to isolate and culture stromal cells have been developed. An array of markers are employed to characterize subtypes of stromal cells, with particular interest in smooth muscle differentiation. RESULTS Defined, serum‐free media are available for certain experimental applications. Conditions that promote smooth muscle differentiation have been identified. Investigators have characterized hormonal and peptide factors that regulate the growth of prostatic stromal cells, and have also described paracrine factors produced by stromal cells that influence epithelial biology. CONCLUSIONS Prostatic stromal‐cell cultures are now widely employed by a large number of investigators for a diverse array of experimental purposes. While further refinement is required to obtain model systems that fully mimic in vivo processes, the availability of stromal‐ and epithelial‐cell cultures provides a valuable resource for studying normal prostatic biology as well as benign prostatic hyperplasia (BPH) and cancer. Prostate 45:115–123, 2000. © 2000 Wiley‐Liss, Inc.

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Differential growth rates in stromal cultures of human prostate derived from patients of varying ages

Cited by 17 publications

References 19 publications

Biological behavior of the gerbil ventral prostate in three phases of postnatal development

Biological behavior of the gerbil ventral prostate in three phases of postnatal development

Stromal and acinar components of the transition zone in normal and hyperplastic human prostate

Cultured stromal cells: An in vitro model of prostatic mesenchymal biology

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