Differential identification of Salmonella enterica subsp. enterica serovar Gallinarum biovars Gallinarum and Pullorum based on polymorphic regions of glgC and speC genes

Kang, Min-Su; Kwon, Yong-Kuk; Jung, Byeong Yeal; Kim, Ae‐Ran; Lee, Kang-Mu; An, Byung-Ki; Song, Eun-A; Kwon, Jun-Hun; Chung, Gab-Soo

doi:10.1016/j.vetmic.2010.05.039

Cited by 43 publications

(35 citation statements)

References 13 publications

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“…The serovars of all strains identified as S. enterica were determined according to the Kauffman-White serotyping scheme (6) with commercial antisera (S & A Reagent Laboratory LMT, Bangkok, Thailand). The identification of S. enterica serovar Gallinarum biovar Gallinarum (S. Gallinarum) and S. Gallinarum biovar Pullorum (S. Pullorum) was based on duplex PCR analysis as described previously (14).…”

Section: Methodsmentioning

confidence: 99%

Prevalence and Fimbrial Genotype Distribution of Poultry Salmonella Isolates in China (2006 to 2012)

Gong

Zhang

et al. 2014

Appl Environ Microbiol

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(39 isolates, 12.1%). Overall, S. Typhimurium was the most commonly detected serovar; among the individual species, S. Pullorum was most commonly isolated from chickens, S. Enteritidis was most common in ducks, S. Typhimurium was most common in geese and pigeons, and S. enterica serovar Saintpaul was most common in turkeys. PCR determination of 20 fimbrial genes demonstrated the presence of bcfD, csgA, fimA, stdB, and sthE genes and the absence of staA and stgA genes in these isolates, and other loci were variably distributed, with frequency values ranging from 11.8 to 99.1%. These 323 Salmonella isolates were subdivided into 41 different fimbrial genotypes, and of these isolate, 285 strains (88.2%) had 12 to 14 fimbrial genes. Our findings indicated that the Salmonella isolates from different poultry species were phenotypically and genetically diverse and that some fimbrial genes are more frequently associated with serovars or serogroups.

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Section: Methodsmentioning

confidence: 99%

Prevalence and Fimbrial Genotype Distribution of Poultry Salmonella Isolates in China (2006 to 2012)

Gong

Zhang

et al. 2014

Appl Environ Microbiol

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“…The presence of each strain was also examined in the liver, spleen and caeca from three birds per each group at 1, 3, 7, 14 and 21 days post-immunization. Specifically, each strain was confirmed by PCR using OMPC primers for the Salmonella genus, SG primers for Salmonella gallinarum, lon and cpxR primers for JOL916, and LTB primers for JOL1355 (Alvarez et al, 2004;Kang et al, 2011;Jeon et al, 2012). Bacterial recovery from liver, spleen and caeca samples was examined as previously described .…”

Section: Safety Examinationmentioning

confidence: 99%

Comparative evaluation of safety and efficacy of a live Salmonella gallinarum vaccine candidate secreting an adjuvant protein with SG9R in chickens

Nandre

Lee

2014

Veterinary Immunology and Immunopathology

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“…In this respect, promising is the method of duplex polymerase chain reaction-based detection of polymorphic sites of genes of isolates. [26] Differential signs of respiratory mycoplasmosis are aerosacculitis, clouding, and thickening of the thoracic and abdominal pneumatic sacs. In the absence of complications by secondary microflora, there may be no signs of injury of parenchymal organs.…”

Section: Discussionmentioning

confidence: 99%

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Sotnikova

2017

AJP

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Aim: The aim of this study is to study pathological processes dynamics at experimental infestation of chickens with Yersinia pseudotuberculosis bacteria. Materials and Methods: Bacteria were cultured on the Hottinger medium. The presence of toxins was assessed using "dilatation of the intestine" and "vascular permeability" tests. For the first test, the investigated liquid with bacteria was administered intranasally, the results were evaluated by comparing the weight difference of lungs, while for the second test the investigated liquid was administered per rectum determining after 4 h the ratio of the weight of the small intestine with the contents to body weight. For morphological studies, histological sections were stained with hematoxylin and eosin. For representative information, preparations were examined under optical microscopy "H604 TrinocularUnico" (USA), stereoscopic microscopy "BIOMED MS-1 Stereo" (Russia), and scanning electron microscopy "Hitachi-800" with scanning add-on device (Japan). Results: At the formation of biofilms consisting of Y. pseudotuberculosis bacteria, we observed the formation of extracellular matrix, which covered the closed structures of various sizes consisting of bacterial cells, followed by subsequent formation of clusters. At the experimental toxemia of chickens, we identified in general exudative-infiltrative processes, the proliferation of antigen-primed lymphocyte, macrophage infiltration of the sinuses of the lymphoid organs, and increasing number of T-lymphocytes. At violation of the porosity in blood vessels of organs of the cardiovascular, respiratory, digestive, and excretory systems, we noted signs of hemocirculation disorders, extensive swelling, liberation of formed elements of blood and the fibrinogenation, dystrophic and necrotic processes, as well as numerous caseous necrotic foci infiltrated by leukocytes, which were revealed in the parenchymatous organs. Conclusion: When culturing Y. pseudotuberculosis bacteria, we observed the intercellular matrix and the formation of biofilms. At experimental toxemia of chickens, the pathological process dynamics was characterized by exudative and infiltrative processes, general vascular reaction, toxic myocardial dystrophy, atrophy of bursa fabricii, dystrophic, and necrotic processes in the Garder glands, Meckel's diverticulum, serous-fibrinous aerosacculitis, broken wind, signs of enterosorption, and hepatorenal syndrome.

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Differential identification of Salmonella enterica subsp. enterica serovar Gallinarum biovars Gallinarum and Pullorum based on polymorphic regions of glgC and speC genes

Cited by 43 publications

References 13 publications

Prevalence and Fimbrial Genotype Distribution of Poultry Salmonella Isolates in China (2006 to 2012)

Prevalence and Fimbrial Genotype Distribution of Poultry Salmonella Isolates in China (2006 to 2012)

Comparative evaluation of safety and efficacy of a live Salmonella gallinarum vaccine candidate secreting an adjuvant protein with SG9R in chickens

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