Differential immune response in mice immunized with the A, R or C domain from Tc<scp>SP</scp> protein of <i>Trypanosoma cruzi</i> or with the coding <scp>DNA</scp>s

Salgado-Jiménez, Berenice; Arce-Fonseca, Minerva; Baylon-Pacheco, Lydia; Talamás-Rohana, Patricia; Rosales‐Encina, José Luis

doi:10.1111/pim.12017

Cited by 11 publications

(8 citation statements)

References 58 publications

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“…The analysis of the IgG subclasses produced by the immunization of canines with both recombinant plasmids revealed that the dominant antibody produced was IgG2; in a previous study in mice immunized with the pBCSP plasmid, the dominant subclass was also IgG2a [26]. These results are consistent with other studies using DNA vaccines; for example, the majority of antibodies produced were of the IgG2 subclass following immunization of dogs with a multi-component DNA vaccine containing T. cruzi trans-sialidase family genes [27].…”

Section: Discussionmentioning

confidence: 99%

Specific humoral and cellular immunity induced by Trypanosoma cruzi DNA immunization in a canine model

Arce-Fonseca

Ballinas-Verdugo

Zenteno

et al. 2013

Vet Res

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Chagas disease has a high incidence in Mexico and other Latin American countries. Because one of the most important known methods of prevention is vector control, which has been effective only in certain areas of South America, the development of a vaccine to protect people at risk has been proposed. In this study, we assessed the cellular and humoral immune response generated following immunization with pBCSP and pBCSSP4 plasmids containing the genes encoding a trans-sialidase protein (present in all three forms of T. cruzi) and an amastigote specific glycoprotein, respectively, in a canine model. Thirty-five beagle dogs were divided randomly into 5 groups (n = 7) and were immunized twice intramuscularly with 500 μg of pBCSSP4, pBCSP, pBk-CMV (empty plasmid) or saline solution. Fifteen days after the last immunization the 4 groups were infected intraperitoneally with 500 000 metacyclic trypomastigotes. The fifth group was unimmunized/infected. The parasitaemia in the immunized/infected dogs was for a shorter period (14 vs. 29 days) and the parasite load was lower. The concentration of IgG1 (0.612 ± 0.019 O.D.) and IgG2 (1.167 ± 0.097 O.D.) subclasses was measured (absorbance) 15 days after the last immunization with both recombinant plasmids, the majority of which were IgG2. The treatment of parasites using the serum from dogs immunized with pBCSP and pBCSSP4 plasmids produced 54% (± 11.8) and 68% (± 21.4) complement-mediated lysis, respectively. At 12 h post immunization, an increase in cytokines was not observed; however, vaccination with pBCSSP4 significantly increased the levels of IFN-γ and IL-10 at 9 months post-infection. The recombinant plasmid immunization stimulated the spleen cell proliferation showing a positive stimulatory index above 2.0. In conclusion, immunization using both genes effectively induces a humoral and cellular immune response.

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Section: Discussionmentioning

confidence: 99%

Specific humoral and cellular immunity induced by Trypanosoma cruzi DNA immunization in a canine model

Arce-Fonseca

Ballinas-Verdugo

Zenteno

et al. 2013

Vet Res

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“…This finding correlates directly with splenic tissue damage during the chronic stage of the disease. Parasite TcSP and TcSSP4 genes encode a trans-sialidase and an amastigote specific-protein, which are expressed in all forms of the parasite and in trypomastigote to amastigote transition forms, respectively, stimulating a strong humoral and cellular response during BALB/c mice infection and preventing pathological manifestations of chronic CD [21,22].…”

Section: Discussionmentioning

confidence: 99%

“…Additionally, survival was 75% in TcSSP4 gene-immunized/infected mice and 100% in those immunized with TcSP gene and then infected. These studies demonstrated protection during the chronic phase of the disease as revealed by the histological analysis showing that immunization diminishes cardiac damage caused by the H8 strain of T. cruzi (MHOM/MX/1992/H8 (T. cruzi)) [21][22][23].…”

Section: Introductionmentioning

confidence: 99%

Effect of the Plasmid-DNA Vaccination on Macroscopic and Microscopic Damage Caused by the Experimental ChronicTrypanosoma cruziInfection in the Canine Model

Rodríguez-Morales

Carrillo-Sánchez

García-Mendoza

et al. 2013

BioMed Research International

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The dog is considered the main domestic reservoir for Trypanosoma cruzi infection and a suitable experimental animal model to study the pathological changes during the course of Chagas disease (CD). Vaccine development is one of CD prevention methods to protect people at risk. Two plasmids containing genes encoding a trans-sialidase protein (TcSP) and an amastigote-specific glycoprotein (TcSSP4) were used as DNA vaccines in a canine model. Splenomegaly was not found in either of the recombinant plasmid-immunized groups; however, cardiomegaly was absent in animals immunized only with the plasmid containing the TcSSP4 gene. The inflammation of subendocardial and myocardial tissues was prevented only with the immunization with TcSSP4 gene. In conclusion, the vaccination with these genes has a partial protective effect on the enlargement of splenic and cardiac tissues during the chronic CD and on microscopic hearth damage, since both plasmids prevented splenomegaly but only one avoided cardiomegaly, and the lesions in heart tissue of dog immunized with plasmid containing the TcSSP4 gene covered only subepicardial tissue.

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“…It is important to mention that rSSP4 induced an antigen-specific immune response, based on the fact that ConA, MPB, or absence of stimuli do not induce this population. Moreover, the induction of Treg cells does not occur by immunization with other T. cruzi -derived recombinant antigens [17]. …”

Section: Discussionmentioning

confidence: 99%

Treg Cells Induced by rSSP4 Derived fromT. cruziAmastigotes Increase Parasitemia in an Experimental Chagas Disease Model

Flores-García

Rosales‐Encina

Rosales-García

et al. 2013

BioMed Research International

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Currently, there is a considerable controversy over the participation of Treg cells during Trypanosoma cruzi infection, the main point being whether these cells play a negative or a positive role. In this work, we found that the adoptive transfer of CD4+CD25+FOXP3+ T cells from rSSP4- (a recombinant Trypanosoma cruzi amastigote derived protein, previously shown to have immunomodulatory properties on macrophages) immunized BALB/c donors into syngenic recipients simultaneously with T. cruzi challenge reduces cardiac inflammation and prolongs hosts' survival but increases blood parasitemia and parasite loads in the heart. These CD4+CD25+FOXP3+ Treg cells from immunized mice have a relatively TGF-β-dependent suppressive activity on CD4+ T cells. Therefore, regulatory CD4+CD25+ T cells play a positive role in the development of acute T. cruzi infection by inducing immunosuppressive activity that controls early cardiac inflammation during acute Chagas disease, prolonging survival, but at the same time promoting parasite growth.

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Differential immune response in mice immunized with the A, R or C domain from TcSP protein of Trypanosoma cruzi or with the coding DNAs

Cited by 11 publications

References 58 publications

Specific humoral and cellular immunity induced by Trypanosoma cruzi DNA immunization in a canine model

Specific humoral and cellular immunity induced by Trypanosoma cruzi DNA immunization in a canine model

Effect of the Plasmid-DNA Vaccination on Macroscopic and Microscopic Damage Caused by the Experimental ChronicTrypanosoma cruziInfection in the Canine Model

Treg Cells Induced by rSSP4 Derived fromT. cruziAmastigotes Increase Parasitemia in an Experimental Chagas Disease Model

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