Differential impact of dose-range glyphosate on locomotor behavior, neuronal activity, glio-cerebrovascular structures, and transcript regulations in zebrafish larvae

Forner-Piquer, Isabel; Faucherre, Adèle; Byram, Julia; Blaquière, Marine; Bock, Frédéric de; Gamet-Payrastre, Laurence; Ellero‐Simatos, Sandrine; Audinat, Etienne; Jopling, Chris; Marchi, Nicola

doi:10.1016/j.chemosphere.2020.128986

Cited by 38 publications

(14 citation statements)

References 63 publications

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“…Morphological parameters were measured at 120 hpf for zebrafish exposed to 0.1, 0.5, 1 and 10 μg/L of cocktail pesticide (duplicate, n=10/group) as we previously described (Forner-Piquer et al, 2020). Mortality rate at 24 hpf and cumulative hatching at 96 and 120 hpf were also evaluated (duplicate, n = 200/group).…”

Section: Zebrafish Locomotor Activitymentioning

confidence: 99%

“…Mortality rate at 24 hpf and cumulative hatching at 96 and 120 hpf were also evaluated (duplicate, n = 200/group). In zebrafish, 120 hpf larvae from control and 10 µg/L cocktail groups were stained using Phalloidin-iFluor 594 (muscle fibers; Abcam ab176757) in duplicate, n=10/group; see (Forner-Piquer et al, 2020). For in vivo 2-photon brain analyses, 96-hpf Tg(fli1a:GFP)y1Tg (duplicate, CTR n=15, 10 μg/L n=10) and Tg(HuC:Tomato) (duplicate, CTR n=10, 10 μg/L n=5) transgenic zebrafish larvae were studied as we previously described (Forner-Piquer et al, 2020).…”

Section: Zebrafish Locomotor Activitymentioning

confidence: 99%

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Varying modalities of perinatal exposure to a pesticide cocktail elicit neurological adaptations in mice and zebrafish

Forner-Piquer

Klément

Gangarossa

et al. 2021

Environmental Pollution

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Highlights 1) Perinatal pesticides links with long-term behavioral modifications in male mice.2) Behavioral changes are accompanied by electrographic brain slowing in vivo.3) In zebrafish larvae, pesticide cocktail elicited dose-dependent motor-behavioral changes.4) In zebrafish larvae, chlorpyriphos is a behavior-modifying pesticide contained in the cocktail.5) Non-lethal prenatal pesticide cocktail does not provoke brain malformations.

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Section: Zebrafish Locomotor Activitymentioning

confidence: 99%

Section: Zebrafish Locomotor Activitymentioning

confidence: 99%

Varying modalities of perinatal exposure to a pesticide cocktail elicit neurological adaptations in mice and zebrafish

Forner-Piquer

Klément

Gangarossa

et al. 2021

Environmental Pollution

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“…There is evidence that this compound can reach surface waters via direct applications and runoffs. Limits for the presence of GLY in drinking water range from the highest levels in Australia (1000 µg/L) and USA (700 µg/L) to lower levels in Canada (280 µg/L) and Europe (0.1 µg/L) [ 2 ]. Despite the claim that GLY-based herbicides only target plant-specific enzymatic pathway, various studies demonstrated adverse effects of this contaminant in both vertebrate and invertebrates [ 3 , 4 , 5 ], as well as in wildlife and aquatic species [ 6 , 7 , 8 , 9 , 10 ], where this contaminant, alone or in formulation, induced oxidative stress and DNA damage.…”

Section: Introductionmentioning

confidence: 99%

Metabolomic and Transcript Analysis Revealed a Sex-Specific Effect of Glyphosate in Zebrafish Liver

Giommi

Ladisa

Carnevali

et al. 2022

IJMS

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Glyphosate is a component of commonly used herbicides for controlling weeds in crops, gardens and municipal parks. There is increasing awareness that glyphosate-based herbicides, in addition to acting on plants, may also exert toxicity in wildlife and humans. In this study, male and female adult zebrafish were exposed to 700 µg/L of glyphosate (GLY), for 28 days. We used the metabolomic approach and UHPLC-ESI-MS to analyze liver samples to investigate the adverse effects of glyphosate on hepatic metabolism. The impact of GLY was found to be sex-specific. In female, GLY exposure affected purine metabolism by decreasing the levels of AMP, GMP and inosinic acid, consequently increasing uric acid levels with respect to the control (CTRL). Exposure to GLY also caused a decrease of UMP levels in the pyrimidine metabolism pathway. In male, GLY exposure decreased the aminoadipic acid within the lysine degradation pathway. Transcript analysis of genes involved in stress response, oxidative stress and the immune system were also performed. Results demonstrated an increased stress response in both sexes, as suggested by higher nr3c1 expression. However, the hsp70.2 transcript level was increased in female but decreased in male. The results demonstrated reduced sod1, sod2, and gpx1a in male following exposure to GLY, indicating an impaired oxidative stress response. At the same time, an increase in the cat transcript level in female was observed. mRNA levels of the pro-inflammatory interleukins litaf and cxcl8b.1 were increased in female. Taken together, the results provide evidence of disrupted nucleotide hepatic metabolism, increased stress inflammatory response in female and disruption of oxidative stress response in male.

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“…47 6 dpf is the earliest timepoint at which zebrafish exhibits maximal spontaneous locomotion. 48 Thus, to assess if SvMEs can relieve this symptom, we performed the behavioral assessment on zebrafish larvae at above time.…”

Section: Resultsmentioning

confidence: 99%

Anti-Parkinson’s Disease Activity of Sanghuangprous vaninii Extracts in the MPTP-Induced Zebrafish Model

Gao

Paudel

et al. 2022

ACS Chem. Neurosci.

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Parkinson’s disease (PD) is a devastating disease of the central nervous system that occurs mainly in the elderly age group, affecting their quality of life. The PD pathogenesis is not yet fully understood and lacks the disease-modifying treatment strategies. Sanghuangprous vaninii (S. vaninii) is a perennial fungus with a plethora of pharmacological activities including anti-cancer and antioxidant activity and so on. However, no study till date has reported its neuroprotective effect against symptoms that are similar to PD in pre-clinical investigation. In the current study, we investigated anti-PD-like effects of S. vaninii mycelium extracts (SvMEs) on MPTP-induced PD in zebrafish. We observed that the loss of dopaminergic neurons and neurovascular reduction were reversed by using SvMEs in the zebrafish brain in a concentration-independent manner. Moreover, it also relieved locomotor impairments in MPTP-induced PD zebrafish. In addition, SvMEs exerted significant antioxidant activity in vitro, which was also demonstrated in vivo on ktr4:NTR-hKikGR zebrafish. Upon investigating the underlying mechanism, we found that SvMEs may alleviate oxidant stress and accelerate α-synuclein degradation and then alleviate PD-like symptoms. Antioxidant-related genes (sod1, gss, gpx4a, gclm, and cat) implied that the SvMEs exhibited anti-PD activity due to the antioxidation mechanism. Finally, upon analysis of chemical composition of SvMEs by liquid chromatography–mass spectrometry, we identified 10 compounds that are plausibly responsible for the anti-PD-like effect of SvMEs. On the limiting part, the finding of the study would have been more robust had we investigated the protein expression of genes related to PD and oxidative stress and compared the effects of SvMEs with any standard anti-PD therapy. Despite this, our results indicated that SvMEs possess anti-PD effects, indicating SvMEs as a potential candidate that is worth exploring further in this avenue.

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Differential impact of dose-range glyphosate on locomotor behavior, neuronal activity, glio-cerebrovascular structures, and transcript regulations in zebrafish larvae

Cited by 38 publications

References 63 publications

Varying modalities of perinatal exposure to a pesticide cocktail elicit neurological adaptations in mice and zebrafish

Varying modalities of perinatal exposure to a pesticide cocktail elicit neurological adaptations in mice and zebrafish

Metabolomic and Transcript Analysis Revealed a Sex-Specific Effect of Glyphosate in Zebrafish Liver

Anti-Parkinson’s Disease Activity of Sanghuangprous vaninii Extracts in the MPTP-Induced Zebrafish Model

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