Differential impact of Holder and High Temperature Short Time pasteurization on the dynamic in vitro digestion of human milk in a preterm newborn model

Nebbia, Stefano; Giribaldi, Marzia; Cavallarin, Laura; Bertino, Enrico; Coscia, Alessandra; Briard‐Bion, Valérie; Ossemond, Jordane; Henry, Gwénaële; Ménard, Olivia; Dupont, Didier; Deglaire, Amélie

doi:10.1016/j.foodchem.2020.127126

Cited by 35 publications

(37 citation statements)

References 34 publications

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“…So far, studies have focused on the impact of storage temperatures and durations on bacterial contamination, immunological components, nutritional quality, reviewed in [20], or metabolite contents and stability [21], and they have been considered for the development of guidelines for the care of breastfeeding mothers and infants [22]. Additionally, pasteurization of HDM was shown to affect bioactive peptide release [23] and further related studies are ongoing [24].…”

Section: Introductionmentioning

confidence: 99%

Impact of Storage Conditions on the Breast Milk Peptidome

et al. 2020

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Human donor milk (HDM) provides appropriate nutrition and offers protective functions in preterm infants. The aim of the study is to examine the impact of different storage conditions on the stability of the human breast milk peptidome. HDM was directly frozen at −80 °C or stored at −20 °C (120 h), 4 °C (6 h), or room temperature (RT for 6 or 24 h). The milk peptidome was profiled by mass spectrometry after peptide collection by ultrafiltration. Profiling of the peptidome covered 3587 peptides corresponding to 212 proteins. The variance of the peptidome increased with storage temperature and time and varied for different peptides. The highest impact was observed when samples were stored at RT. Smaller but significant effects were still observed in samples stored at 4 °C, while samples showed highest similarity to those immediately frozen at −80 °C when stored at −20 °C. Peptide structures after storage at RT for 24 h point to the increased activity of thrombin and other proteases cleaving proteins at lysine/arginine. The results point to an ongoing protein degradation/peptide production by milk-derived proteases. They underline the need for immediate freezing of HDM at −20 °C or −80 °C to prevent degradation of peptides and enable reproducible investigation of prospectively collected samples.

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Section: Introductionmentioning

confidence: 99%

Impact of Storage Conditions on the Breast Milk Peptidome

et al. 2020

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“…The most common pasteurization process employed, Holder method (30 min at 62.5 • C), denatures many of the bioactive proteins found in HM [61], including BSSL [62], often resulting in suboptimal digestion of lipids by the neonate [63]. In addition, heat from pasteurization threatens the integrity of the donor MFGM, as phospholipids may be released from the outer lipid bilayer [64] and protein aggregates often accumulate at the surface of the MFGM [65]. The effects of phospholipid release and surface protein aggregation alter the size of the MFG, thus influencing lipase kinetics.…”

Section: Structural Compositional and Digestive Differences Of Lipimentioning

confidence: 99%

“…The unique composition and structure of the human MFG have proven difficult to mimic in infant formula [46] and are often disrupted by processing methods associated with DM [65]. Complex lipids, located both within the MFGM and in membranes of HM exosomes, comprise up to 1% of the lipids found within HM [41].…”

Section: Complex Lipidsmentioning

confidence: 99%

Lipid Composition, Digestion, and Absorption Differences among Neonatal Feeding Strategies: Potential Implications for Intestinal Inflammation in Preterm Infants

et al. 2021

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Necrotizing enterocolitis (NEC) is a significant cause of morbidity and mortality in the neonatal population. Formula feeding is among the many risk factors for developing the condition, a practice often required in the cohort most often afflicted with NEC, preterm infants. While the virtues of many bioactive components of breast milk have been extolled, the ability to digest and assimilate the nutritional components of breast milk is often overlooked. The structure of formula differs from that of breast milk, both in lipid composition and chemical configuration. In addition, formula lacks a critical digestive enzyme produced by the mammary gland, bile salt-stimulated lipase (BSSL). The gastrointestinal system of premature infants is often incapable of secreting sufficient pancreatic enzymes for fat digestion, and pasteurization of donor milk (DM) has been shown to inactivate BSSL, among other important compounds. Incompletely digested lipids may oxidize and accumulate in the distal gut. These lipid fragments are thought to induce intestinal inflammation in the neonate, potentially hastening the development of diseases such as NEC. In this review, differences in breast milk, pasteurized DM, and formula lipids are highlighted, with a focus on the ability of those lipids to be digested and subsequently absorbed by neonates, especially those born prematurely and at risk for NEC.

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“…In relation to sterilized human milk, EPA reduced 1.5%, DPA 3.8%, DHA 2.7%, di-homolinolenic acid 2.1%, DHA 1.6% and AA 5.3%, thus showing that sterilization led to a greater reduction in the values of fatty acids. Nebbia et al (2020) studied HTST (72 °C for 15 seconds) as an alternative to Holder pasteurization (62.5 °C for 30 minutes) to increase the preservation of bioactive compounds in human milk, noting that there was an increase in proteins on the surface of blood cells fat after pasteurization, to a lesser extent in HTST than in Holder pasteurization. Parrón et al (2018) evaluated in their study different pasteurization treatments, such as standard (62.5 C for 30 minutes), HTST (75 ° C for 20 seconds) and high pressure (600 MPa for 15 minutes) in human milk, on its components such as immunoglobulin A (IgA), lactoferrin, mucins and lactaderine.…”

Section: High Temperature and Short Time (Htst)mentioning

confidence: 99%

Human milk: processing and conservation – a review

Castro

Alves

Saqueti

et al. 2021

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Human milk has a balance of nutrients and bioactive compounds, and it must be the exclusive food source during the first six months of the infant's life. In cases where the woman is unable to breastfeed, due to hypogalactia or pre-existing disease, the use of donated human milk, available in human milk banks, is recommended. Currently, in the human milk banks the processing applied for greater conservation is Holder pasteurization. Therefore, the objective of this work is to perform a search in the literature about new possible treatments to be applied to human milk, and its influence on its physical-chemical composition; and in this sense, the studied processes were: Holder pasteurization, lyophilization, spray-drying, high hydrostatic pressure, high temperature and short time, and short wave ultraviolet irradiation. For that, a search was performed in databases, and 126 articles were selected to carry out this review according to the keywords used. It was observed that the studied treatments can be applied for the processing and conservation of human milk, once it was applied in matrices with similar composition.

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Differential impact of Holder and High Temperature Short Time pasteurization on the dynamic in vitro digestion of human milk in a preterm newborn model

Cited by 35 publications

References 34 publications

Impact of Storage Conditions on the Breast Milk Peptidome

Impact of Storage Conditions on the Breast Milk Peptidome

Lipid Composition, Digestion, and Absorption Differences among Neonatal Feeding Strategies: Potential Implications for Intestinal Inflammation in Preterm Infants

Human milk: processing and conservation – a review

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