Differential inflammatory responses of bovine foot skin fibroblasts and keratinocytes to digital dermatitis treponemes

Evans, Nicholas J.; Brown, Jennifer M.; Scholey, Rachel; Murray, Richard D.; Birtles, Richard J.; Carter, Stuart

doi:10.1016/j.vetimm.2014.05.005

Cited by 21 publications

(37 citation statements)

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“…The balance between MMPs and TIMPs is an important equilibrium for common physiologic functions, and it can also be responsible for the nature of proteolytic events either in the direction of tissue remodelling or breakage during wound healing, resulting in ulceration. Inflammatory gene expression profiles of bovine dermal fibroblasts and keratinocytes after challenge with Treponemal wall fragments have been investigated in a recent in vitro study on BDD (Evans et al 2014). In this study, upregulation of many genes, including MMP-12), has been reported in cultured dermal fibroblasts, but not in keratinocytes.…”

Section: Introductionmentioning

confidence: 81%

“…Bovine digital dermatitis (BDD) is an important foot disease commonly observed in dairy cattle (Evans et al 2014). BDD presents several phases with different effects, even less obvious or painful.…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, it is possible to conclude that the hypoxia facilitates the growth of anaerobic bacteria which are involved in the pathogenesis of BDD infection and can also engender important changes in cellular levels on the expression of various proteins (MMPs, growth factors, chemokines, etc.) which may be important in the skin biology of the foot (Evans et al 2014). In many premises, bovine hind feet recurrently contacts with manure; and it is also known that continuous moisture and low oxygen tension are present on the hind feet of cattle if manure removal and hygiene is not adequate (Berry 2001).…”

Section: Introductionmentioning

confidence: 99%

“…Matrix metalloproteinases (MMPs) are zinc-dependent proteases responsible for the degradation of extracellular matrix proteins such as gelatin, collagen, laminin and fibronectin. MMPs are synthesized by a wide variety of cells, including fibroblasts (Nagase & Woessner 1999;Toriseva et al 2012;Evans et al 2014). Their importance and contributions in both skin wound healing and formation of ulcerative lesions were mainly emphasized in humans (Trengove et al 1999;Rayment et al 2008;Subramaniam et al 2008).…”

Section: Introductionmentioning

confidence: 99%

“…After hypoxic treatment, elevated level of MMP-1, MMP-2 and MMP-13 proteins in various cultured cell types (including fibroblasts) in humans was reported (Yamanaka & Ishikawa 2000;Kan et al 2003;Lolmede et al 2003;Leufgen et al 2005). According to the best of authors' knowledge, there is only one study about the involvement of MMP enzymes in the pathogenesis of BDD in bovine dermal fibroblast (Evans et al 2014). It is clear from their study that MMP-12 enzyme was strongly upregulated (up to nine folds) when bovine dermal fibroblasts exposed to various Treponemal cell sonicates (bacterial fragments).…”

Section: Introductionmentioning

confidence: 99%

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Hypoxia enhances secretion of matrix metalloproteinases in bovine dermal fibroblasts: an in vitro approach to bovine digital dermatitis

Akkoç

Kahraman

Mutlu

2017

Journal of Applied Animal Research

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Dermal fibroblasts are of great importance in skin homeostasis. They are responsible for the synthesis and remodelling of extracellular proteins by the way of synthesis of some specific kind of Matrix Metalloproteinases (MMPs). Imbalance between MMPs and their tissue inhibitors (TIMPs) are known to involve ulcerative wounds in many tissues, including skin. In veterinary medicine, bovine digital dermatitis (BDD) is an ulcerative skin disorder of dairy cattle. To date, Koch's postulate has not yet been fulfilled for the pathogenesis. In present study, we tested in vitro role of hypoxia if it evokes a response in MMP-1, MMP-2 and MMP-13 enzymes and their inhibitors in bovine dermal fibroblasts. Dermal fibroblasts were exposed to hypoxia in various time periods (6, 12 and 24 h). Immunoblotting revealed 6-, 12-and 24-h hypoxia, which resulted in 43.5%, 75% and 65% increase in MMP-1, 76%, 61% and 55% increase in MMP-2 and 80%, 78% and 55% increase in MMP-13 enzymes levels, respectively. We observed slight increases in TIMP-1 and TIMP-2 enzymes, but differences between treatment and control groups did not show any statistical significance. Our findings indicate that hypoxia induces MMP-1, MMP-2 and MMP-13 enzymes and may contribute to pathogenesis of BDD through in the development of in vivo lesions. ARTICLE HISTORY

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Section: Introductionmentioning

confidence: 81%

Section: Introductionmentioning

confidence: 99%