2015
DOI: 10.4303/jdar/235928
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Differential Lipid Profiles in Experimental Steatohepatitis: Role for Imaging Mass Spectrometry as a Diagnostic Aid

Abstract: Background. Ethanol, tobacco-specific nitrosamine ketone (NNK), and ethanol+NNK exposures cause steatohepatitis, suggesting that smoking can be a cofactor in alcoholic liver disease. Study design. We used MALDI-TOF imaging mass spectrometry (IMS) to characterize hepatic lipid profiles in steatohepatitis caused by ethanol, NNK, and ethanol+NNK. Results. Ethanol, NNK, and ethanol+NNK increased levels and altered the profiles of hepatic phospholipids. Ethanol caused striking accumulations of m/z 932.7 phosphatidy… Show more

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Cited by 5 publications
(19 citation statements)
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“…The tissue disks were cryo-embedded in 2% sodium carboxymethylcellulose (CMC). Cryosections (8 μm) were thaw-mounted onto indium tin oxide (ITO)-coated slides (Delta Technologies, Loveland, CO) and coated by sublimation with 2,5-dehydroxybenzoic acid (DHB; Sigma-Aldrich Co, St. Louis, MO) as matrix (Angel, Spraggins, Baldwin, & Caprioli, 2012; Emine B. Yalcin, Nunez, Cornett, & de la Monte, 2015; E.B. Yalcin, Nunez, Tong, Cornett, & de la Monte, 2015).…”
Section: Methodsmentioning
confidence: 99%
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“…The tissue disks were cryo-embedded in 2% sodium carboxymethylcellulose (CMC). Cryosections (8 μm) were thaw-mounted onto indium tin oxide (ITO)-coated slides (Delta Technologies, Loveland, CO) and coated by sublimation with 2,5-dehydroxybenzoic acid (DHB; Sigma-Aldrich Co, St. Louis, MO) as matrix (Angel, Spraggins, Baldwin, & Caprioli, 2012; Emine B. Yalcin, Nunez, Cornett, & de la Monte, 2015; E.B. Yalcin, Nunez, Tong, Cornett, & de la Monte, 2015).…”
Section: Methodsmentioning
confidence: 99%
“…The sections were imaged in the negative ion mode using a reflectron geometry MALDI-time-of-flight (TOF)/TOF mass spectrometer (Ultraflextreme, Bruker Daltonics, Bremen, Germany), and analyzed by focusing a Smartbeam II Nd:YAG laser onto ~100 μm 2 areas of white matter (Jackson, Wang, & Woods, 2007; Emine B. Yalcin et al, 2015; E.B. Yalcin et al, 2015).…”
Section: Methodsmentioning
confidence: 99%
“…STZ (n=7) brains (Table 1). Lipid assignments were made by comparing the precursor and product ion m/z values with those catalogued in the LIPID MAPS database and confirmed by MS/MS as previously described (Yalcin et al, 2015a; Yalcin et al, 2015b). Alternatively, the lipid ion assignments were made based on published reports (Dreisewerd et al, 2007; Eckhardt et al, 2007; Fernandez-Lima et al, 2011; Gode and Volmer, 2013; Hsu and Turk, 2000; Shanta et al, 2011).…”
Section: Resultsmentioning
confidence: 99%
“…Cryostat sections (10 μm thick) were thaw mounted onto indium tin oxide (ITO) coated glass slides (Delta Technologies, Loveland, CO) and prepared for lipid analysis by sublimation coating with 200 ± 13 mg/cm 2 2,5-dehydroxybenzoic acid (DHB; Sigma-Aldrich Co, St. Louis, MO) as a thin uniform layer of matrix (Angel et al, 2012; Yalcin and de la Monte, 2015). The tissue sections were dehydrated for 1 hour in a vacuum desiccator, then washed 5 times with 50 mM, pH 6.4 ammonium formate (150 μl/slide/wash-5 sec each) to remove salts and enhance sensitivity during spectra acquisition (Yalcin et al, 2015a; Yalcin et al, 2015b). The slides were again dried in a vacuum dessicator for 30 minutes.…”
Section: Methodsmentioning
confidence: 99%
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