Differential modulation of ochratoxin A absorption across Caco-2 cells by dietary polyphenols, used at realistic intestinal concentrations

Sergent, Thérèse; Garsou, Serge; Schaut, Annelore; Saeger, Sarah De; Pussemier, Luc; Peteghem, Carlos Van; Larondelle, Yvan; Schneider, Yves‐Jacques

doi:10.1016/j.toxlet.2005.04.013

Cited by 72 publications

(38 citation statements)

References 44 publications

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“…By also using MK571, Henry et al (2005) have reported the implication of MRP2/ MRP3 in human intestinal Caco2 cell line transport of conjugated resveratrol. Moreover, it was shown that resveratrol impairs ochratoxin A intestinal efflux in a manner similar to that of MK571, suggesting a competitive inhibition of MRP-2 by resveratrol (Sergent et al, 2005). Conversely, we have not shown any effect of verapamil on resveratrol efflux, excluding the implication of the multidrug resis- FIG.…”

Section: Ion-trap Mass Spectrometry Analysis Of Resveratrol Metabolitesmentioning

confidence: 59%

Resveratrol in Human Hepatoma HepG2 Cells: Metabolism and Inducibility of Detoxifying Enzymes

Lançon¹,

Hanet²,

Jannin³

et al. 2007

Drug Metabolism and Disposition

108

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ABSTRACT:trans-Resveratrol is a polyphenol present in several plant species. Its chemopreventive properties against several diseases have been largely documented. To validate a model for the study of the factors influencing its biological fate at the hepatic level, the metabolism and the efflux of resveratrol were studied in the human hepatoblastoma cell line, HepG2. Comparative high-performance liquid chromatography analysis of cell culture media before and after deconjugation showed that resveratrol was rapidly conjugated; at the concentration of 10 M, it was entirely metabolized at 8 h of incubation. Two main resveratrol metabolites, monosulfate and disulfate, were identified by atmospheric pressure chemical

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Section: Ion-trap Mass Spectrometry Analysis Of Resveratrol Metabolitesmentioning

confidence: 59%

Resveratrol in Human Hepatoma HepG2 Cells: Metabolism and Inducibility of Detoxifying Enzymes

Lançon¹,

Hanet²,

Jannin³

et al. 2007

Drug Metabolism and Disposition

108

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“…However, Sergent et al (2005) found a slight cytotoxic effect of RES on Caco-2 cells after 48 h of treatment at 100 lM.…”

Section: Discussionmentioning

confidence: 94%

“…In fact, co-exposure of OTA at concentrations of 40 lM (moderately toxic dose) with the highest concentration of RES (100 lM) resulted in an increase in cytotoxicity. Sergent et al (2005) showed that the Caco-2 absorption of OTA, at concentrations that should be easily encountered in the gut, is increased in the presence of RES. This would imply a greater bioavailability of the mycotoxin which could increase the toxicity of this compound.…”

Section: Discussionmentioning

confidence: 99%

Cytotoxicity of the mycotoxins deoxynivalenol and ochratoxin A on Caco-2 cell line in presence of resveratrol

Cano-Sancho¹,

González-Arias²,

Ramos³

et al. 2015

Toxicology in Vitro

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Exposure to mycotoxins through dietary food intake involves a highly complex scenario where co-contamination of different mycotoxins has been frequently demonstrated. On the other hand, the effect of the interaction of mycotoxins with other generally considered beneficial food components, as the antioxidants, has been scarcely studied. The main goal of the present work was to assess the cytotoxic effects on Caco-2 cells of the mycotoxins deoxynivalenol (DON) and ochratoxin A (OTA), alone or combined, and to explore potential protective effects of resveratrol (RES), an antioxidant frequently found in wine. In parallel, reactive oxygen species (ROS) production has also been studied as a first approach to understand the underlying mechanism of cytotoxicity. Results indicate a higher toxic effect of the mycotoxins when they are co-exposed. This increase in cytotoxicity was not accompanied by an increase in ROS production. The co-exposure of OTA or DON with RES did not result in a decrease in cytotoxicity; on the contrary, it resulted in increased cytotoxicity not associated with an increase in ROS production.

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“…Recently published papers suggest that some of these polyphenols may modify or modulate the uptake of the toxin by several mechanisms, for example by inhibition of OAT [27,28] and OATP [29]. It also should be noted that adverse effects of some flavonoids occur by inhibiting ATP binding cassette (ABC) transport proteins with consequent blocking of the efflux mechanisms of OTA from cells [30][31][32]. On the other hand it is well known that certain flavonoids bind to albumin with high affinity: subdomain IIA (site I) was defined as the binding site in several cases [33][34][35].…”

Section: Introductionmentioning

confidence: 99%

Flavonoid aglycones can compete with Ochratoxin A for human serum albumin: A new possible mode of action

Poór

Kunsági‐Máté

Bencsik

et al. 2012

International Journal of Biological Macromolecules

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Differential modulation of ochratoxin A absorption across Caco-2 cells by dietary polyphenols, used at realistic intestinal concentrations

Cited by 72 publications

References 44 publications

Resveratrol in Human Hepatoma HepG2 Cells: Metabolism and Inducibility of Detoxifying Enzymes

Resveratrol in Human Hepatoma HepG2 Cells: Metabolism and Inducibility of Detoxifying Enzymes

Cytotoxicity of the mycotoxins deoxynivalenol and ochratoxin A on Caco-2 cell line in presence of resveratrol

Flavonoid aglycones can compete with Ochratoxin A for human serum albumin: A new possible mode of action

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