Differential modulation of the functionality of white adipose tissue of obese Zucker (fa/fa) rats by the type of protein and the amount and type of fat

Dı́az-Villaseñor, Andrea; Granados, Omar; González-Palacios, Berenice; Tovar‐Palacio, Claudia; Torre-Villalvazo, Iván; Olivares-García, Verónica; Torres, Nimbe; Tovar, Armando R.

doi:10.1016/j.jnutbio.2013.03.007

Cited by 13 publications

(25 citation statements)

References 63 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Even though the endocrine characteristics of the Zucker ( fa/fa ) rats are typical of obesity, metabolic syndrome and type 2 diabetes, we have demonstrated that the consumption of soy protein reduces circulating glucagon levels to near normal values in wild‐type rats, sensitizing the lipolytic pathway in WAT . In the present study, we demonstrated that this effect occurs in part by a mechanism responsible for increasing the GR at the plasma membrane of adipocytes.…”

Section: Discussionmentioning

confidence: 45%

“…More recently, we found that soy protein decreases fasting hyperglucagonemia in obese Zucker ( fa/fa ) rats by approximately 51% . Glucagon concentration in the rats fed soy protein decreased from approximately 228.8 ± 12.5 ng/L to 111± 7.8 ng/L , reaching values similar to those observed in fasting rats without genetic alterations .…”

Section: Introductionmentioning

confidence: 51%

“…B). Thus, these results could imply that the reduction in hyperglucagonemia previously observed after soy protein consumption in Zucker ( fa/fa) rats may be due to the cellular localization of the GR rather than a modification in its total protein amount.…”

Section: Resultsmentioning

confidence: 77%

“…In our previous work , we showed that soy protein increased the proportion of HSL phosphorylated at Ser563 by PKA with respect to the inhibitory phosphorylation at Ser565 by AMPK , beside the dramatic reduction in serum glucagon levels. As a result, serum glycerol concentration, which is used as a lipolysis marker, increased in rats fed a soy protein diet containing a normal dietary fat content, suggesting that rats fed a soy protein diet have a more sensitive HSL signaling pathway compared with those fed a casein diet ; however, the mechanism of this adaptation is still unclear.…”

Section: Introductionmentioning

confidence: 91%

See 3 more Smart Citations

Recycling of glucagon receptor to plasma membrane increases in adipocytes of obese rats by soy protein; implications for glucagon resistance

Velázquez-Villegas

Tovar‐Palacio

Palacios‐González

et al. 2017

Molecular Nutrition Food Res

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 45%

Section: Introductionmentioning

confidence: 51%

Section: Resultsmentioning

confidence: 77%

Section: Introductionmentioning

confidence: 91%

See 2 more Smart Citations

Recycling of glucagon receptor to plasma membrane increases in adipocytes of obese rats by soy protein; implications for glucagon resistance

Velázquez-Villegas

Tovar‐Palacio

Palacios‐González

et al. 2017

Molecular Nutrition Food Res

Self Cite

View full text Add to dashboard Cite

show abstract

“…All primers were synthesized by Sigma Aldrich, Austria. Hypothetical mRNA starting concentrations were calculated using LinReg v12.8, normalized to hypoxanthine guanine phosphoribosyl transferase ( HPRT1 ) as endogenous control (Han et al, 2002; Diaz-Villasenor et al, 2013; Rohm et al, 2015; Holik et al, 2016) and are given as fold change to vehicle control treated cells (=1).…”

Section: Methodsmentioning

confidence: 99%

The Alkamide trans-Pellitorine Targets PPARγ via TRPV1 and TRPA1 to Reduce Lipid Accumulation in Developing 3T3-L1 Adipocytes

et al. 2017

View full text Add to dashboard Cite

Adipose tissue is an important endocrine organ in the human body. However, pathological overgrowth is associated with chronic illness. Regulation of adipogenesis and maturation of adipocytes via bioactive compounds in our daily diet has been in focus of research in the past years and showed promising results for agonists of the ion channels transient receptor potential channel (TRP) V1 and A1. Here, we investigated the anti-adipogenic potential and underlying mechanisms of the alkamide trans-pellitorine present in Piper nigrum via TRPV1 and TRPA1 in 3T3-L1 cells. trans-pellitorine was found to suppress mean lipid accumulation, when applied during differentiation and maturation, but also during maturation phase solely of 3T3-L1 cells in a concentration range between 1 nM and 1 μM by up to 8.84 ± 4.97 or 7.49 ± 5.08%, respectively. Blockage of TRPV1 using the specific inhibitor trans-tert-butyl-cyclohexanol demonstrated that the anti-adipogenic activity of trans-pellitorine depends on TRPV1. In addition, blockage of the TRPA1 channel using the antagonist AP-18 showed a TRPA1-dependent signaling in the early to intermediate stages of adipogenesis. On a mechanistic level, treatment with trans-pellitorine during adipogenesis led to reduced PPARγ expression on gene and protein level via activation of TRPV1 and TRPA1, and increased expression of the microRNA mmu-let-7b, which has been associated with reduced PPARγ levels. In addition, cells treated with trans-pellitorine showed decreased expression of the gene encoding for fatty acid synthase, increased expression of microRNA-103 and a decreased short-term fatty acid uptake on the functional level. In summary, these data point to an involvement of the TRPV1 and TRPA1 cation channels in the anti-adipogenic activity of trans-pellitorine via microRNA-let7b and PPARγ. Since trans-pellitorine does not directly activate TRPV1 or TRPA1, an indirect modulation of the channel activity is assumed and warrants further investigation.

show abstract

Nonivamide Enhances miRNA let‐7d Expression and Decreases Adipogenesis PPARγ Expression in 3T3‐L1 Cells

Rohm

Holik²,

Kretschy³

et al. 2015

J of Cellular Biochemistry

View full text Add to dashboard Cite

Red pepper and its major pungent principle, capsaicin (CAP), have been shown to be effective anti‐obesity agents by reducing energy intake, enhancing energy metabolism, decreasing serum triacylglycerol content, and inhibiting adipogenesis via activation of the transient receptor potential cation channel subfamily V member 1 (TRPV1). However, the binding of CAP to the TRPV1 receptor is also responsible for its pungent sensation, strongly limiting its dietary intake. Here, the effects of a less pungent structural CAP‐analog, nonivamide, on adipogenesis and underlying mechanisms in 3T3‐L1 cells were studied. Nonivamide was found to reduce mean lipid accumulation, a marker of adipogenesis, to a similar extent as CAP, up to 10.4% (P < 0.001). Blockage of the TRPV1 receptor with the specific inhibitor trans‐tert‐butylcyclohexanol revealed that the anti‐adipogenic activity of nonivamide depends, as with CAP, on TRPV1 receptor activation. In addition, in cells treated with nonivamide during adipogenesis, protein levels of the pro‐adipogenic transcription factor peroxisome‐proliferator activated receptor γ (PPARγ) decreased. Results from miRNA microarrays and digital droplet PCR analysis demonstrated an increase in the expression of the miRNA mmu‐let‐7d‐5p, which has been associated with decreased PPARγ levels. J. Cell. Biochem. 116: 1153–1163, 2015. © 2015 The Authors. Journal of Cellular Biochemistry published by Wiley Periodicals, Inc.

show abstract

Differential modulation of the functionality of white adipose tissue of obese Zucker (fa/fa) rats by the type of protein and the amount and type of fat

Cited by 13 publications

References 63 publications

Recycling of glucagon receptor to plasma membrane increases in adipocytes of obese rats by soy protein; implications for glucagon resistance

Recycling of glucagon receptor to plasma membrane increases in adipocytes of obese rats by soy protein; implications for glucagon resistance

The Alkamide trans-Pellitorine Targets PPARγ via TRPV1 and TRPA1 to Reduce Lipid Accumulation in Developing 3T3-L1 Adipocytes

Nonivamide Enhances miRNA let‐7d Expression and Decreases Adipogenesis PPARγ Expression in 3T3‐L1 Cells

Contact Info

Product

Resources

About