Differential morphometric and micro-morpho-anatomical responses toward types of culture vessels used in micropropagation of Hemidesmus indicus (L.) R. Br.

Manokari, M.; Badhepuri, Mahesh Kumar; Cokulraj, M.; Dey, Abhijit; Rajput, Vishnu D.; Minkina, Tatiana; Shekhawat, Mahipal S.

doi:10.1007/s11240-021-02189-x

Cited by 6 publications

(3 citation statements)

References 21 publications

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“…Addition of BAP to the medium induced a lower palisade/spongy ratio, mainly in the absence of KIN. This decreased palisade/spongy ratio can indicate a reduced development level of leaf anatomy, which means a larger area with larger interstitial spaces among parenchyma cells (Luan et al 2021;Manokari et al 2022).…”

Section: Discussionmentioning

confidence: 99%

“…Morphophysiological disorders might be a stress response and can be characterized by studies that connect the anatomy, physiology, and biochemistry of in vitro explants. Analyses involving the quantitative stem and leaf anatomy of shoots are valuable tools for de ning how in vitro conditions can in uence the success of all micropropagation steps (Martins et al 2015;Manokari et al 2021bManokari et al , 2022). In addition to anatomical studies, a deeper approach to physiological and biochemical changes may be useful for stress characterization.…”

Section: Introductionmentioning

confidence: 99%

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6-Benzylaminopurine and kinetin modulations during in vitro propagation of Quercus robur (L.): an assessment of anatomical, biochemical, and physiological profiling of shoots

Martins

Wawrzyniak

Ley-López

et al. 2022

Plant Cell Tiss Organ Cult

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For many woody species, such as Quercus robur, cytokinins in the culture medium are required to maintain in vitro plant material. Among synthetic cytokinins, 6-benzylaminopurine (BAP) and kinetin (KIN) are the most frequently used. In addition to inducing shoots, cytokinins can cause morphophysiological disorders. Therefore, we aimed to investigate the anatomical, biochemical, and physiological alterations and pro les of Q. robur shoots exposed to two cytokinins, applied alone and in combination. Shoots previously established in vitro were transferred to WPM culture media supplemented with BAP at concentrations of 0, 1.25, and 3.50 µM combined with KIN at concentrations of 0, 0.62, and 1.25 µM totaling 9 treatments. Anatomical, physiological, and biochemical analyses were performed after 40 d of culture. BAP induced the formation of new buds with anatomically underdeveloped leaves; induced shoot-tip necrosis, which is considered a response to the ine cient transport of water and nutrients; reduced the thickness of the cell walls of phloem bers; and decreased the content of phenolic compounds and photosynthetic pigments. These responses were less pronounced with co-exposure to KIN. In contrast, KIN alone stimulated a larger area of secondary xylem and more ligni ed cell walls. BAP can induce shoots with underdeveloped anatomical and biochemical characteristics. Shoots that grew with KIN alone had stem and leaf anatomical characteristics, indicating greater commitment to cellular differentiation than proliferation. When both cytokinins are combined, KIN can partially mitigate the deleterious effects of BAP on in vitro growth.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

6-Benzylaminopurine and kinetin modulations during in vitro propagation of Quercus robur (L.): an assessment of anatomical, biochemical, and physiological profiling of shoots

Martins

Wawrzyniak

Ley-López

et al. 2022

Plant Cell Tiss Organ Cult

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“…Conical flask improved shoot proliferation, quality, aerial tissue system and ex vitro development of in vitro plantlets than culture bottles [57] 9.…”

Section: Culture Bottle Conical Flaskmentioning

confidence: 90%

Effect of microclimatic physical factors on in vitro morphogenesis of plants: a systematic review

Gautam,

Faroda,

Agarwal

et al. 2024

Discov Agric

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Biotechnology has revolutionized plant propagation by providing plant tissue culture as a rising alternative platform to conventional methods. In the present scenario, plant tissue culture becomes the key technique for the large-scale production and conservation of germplasm, maintenance of genetic stability, and meets out the economic demands of plant and plant products. It is the science of artificially growing test tube plantlets under optimized microclimatic conditions isolated from the external environment. This micro-scale environment resembles the natural environment consisting of culture vessels, humidity, gases, light irradiation, spectrum, temperature, nutritional media, and its constituents as major growth-affecting factors. The efficient architectural development of any genotype depends on these factors and requires a specifically optimized climate. Here, we are reviewing the interaction of physical factors of in vitro artificial climate including culture vessel, light, and temperature with the developing plantlets. The outcomes can increase the understanding of the developmental response of plants toward their environment in the recently changing climatic conditions. Further, this can also eliminate the challenges of cost, labor, and timings and introduce the advancements of efficient large-scale production of plant and plant products which will be effective in fulfilling the population demands.

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Biotechnological interventions and indole alkaloid production in Rauvolfia serpentina

Dey

Roy²,

Mohture³

et al. 2022

Appl Microbiol Biotechnol

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Differential morphometric and micro-morpho-anatomical responses toward types of culture vessels used in micropropagation of Hemidesmus indicus (L.) R. Br.

Cited by 6 publications

References 21 publications

6-Benzylaminopurine and kinetin modulations during in vitro propagation of Quercus robur (L.): an assessment of anatomical, biochemical, and physiological profiling of shoots

6-Benzylaminopurine and kinetin modulations during in vitro propagation of Quercus robur (L.): an assessment of anatomical, biochemical, and physiological profiling of shoots

Effect of microclimatic physical factors on in vitro morphogenesis of plants: a systematic review

Biotechnological interventions and indole alkaloid production in Rauvolfia serpentina

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