Differential permeation of piroxicam-loaded PLGA micro/nanoparticles and their in vitro enhancement

Shankarayan, Raju; Kumar, Sumit; Mishra, Prashant

doi:10.1007/s11051-013-1496-6

Cited by 7 publications

(3 citation statements)

References 48 publications

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“…In the last decade, the use of PLGA has been investigated to deliver a wide spectrum of active agents, from hydrophobic drug molecules [6][7][8] to hydrophilic biomolecules as peptides [9], proteins [10][11][12][13][14][15] or nucleic acids [16,17]. These delivery systems have been produced via different formulation processes for their application in both systemic and local site-specific therapies [18].…”

Section: Introductionmentioning

confidence: 99%

Dual delivery nanosystem for biomolecules. Formulation, characterization, and in vitro release

Ortega-Oller

Castillo-Santaella

Padial‐Molina

et al. 2017

Colloids and Surfaces B: Biointerfaces

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Because of the biocompatible and biodegradable properties of poly (lactic-co-glycolic acid) (PLGA), nanoparticles (NPs) based on this polymer have been widely studied for drug/biomolecule delivery and long-term sustained-release. In this work, two different formulation methods for lysozyme-loaded PLGA NPs have been developed and optimized based on the double-emulsion (water/oil/water, W/O/W) solvent evaporation technique. They differ mainly in the phase in which the surfactant (Pluronic F68) is added: water (W-F68) and oil (O-F68). The colloidal properties of these systems (morphology by SEM and STEM, hydrodynamic size by DLS and NTA, electrophoretic mobility, temporal stability in different media, protein encapsulation, release, and bioactivity) have been analyzed. The interaction surfactant-protein depending on the formulation procedure has been characterized by surface tension and dilatational rheology. Finally, cellular uptake by human mesenchymal stromal cells and cytotoxicity for both systems have been analyzed. Spherical hard NPs are made by the two methods However, in one case, they are monodisperse with diameters of around 120nm (O-F68), and in the other case, a polydisperse system of NPs with diameters between 100 and 500nm is found (W-F68). Protein encapsulation efficiency, release and bioactivity are maintained better by the W-F68 formulation method. This multimodal system is found to be a promising "dual delivery" system for encapsulating hydrophilic proteins with strong biological activity at the cell-surface and cytoplasmic levels.

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Section: Introductionmentioning

confidence: 99%

Dual delivery nanosystem for biomolecules. Formulation, characterization, and in vitro release

Ortega-Oller

Castillo-Santaella

Padial‐Molina

et al. 2017

Colloids and Surfaces B: Biointerfaces

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“…The use of nanocarriers could 20 improve the drug oral bioavailability, therapeutic efficacy and 21 safety profile, since the encapsulated drug is masked in the 22 nanostructure. Nanoparticles could prevent the direct contact of 23 the drug with the mucosa, protect the molecule from degradation 24 in the gastric environment (Shankarayan et al, 2013), or by-pass 25 the cell efflux pumps. These are key players in the multidrug 26 resistance of tumors (Deli, 2009).…”

mentioning

confidence: 99%

Ex vivo permeation of tamoxifen and its 4-OH metabolite through rat intestine from lecithin/chitosan nanoparticles

Barbieri

Buttini

Rossi

et al. 2015

International Journal of Pharmaceutics

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“…The absorbance of resulting supernatant was taken at respective wavelengths and concentration was calculated from the standard curve. All absorbance values were recorded in triplicates and the entrapment efficiency was calculated using the following equation, where the fraction is defined as actual loading (μg/mg) of compounds [ 36 ]…”

Section: Methodsmentioning

confidence: 99%

Gelsolin Amyloidogenesis Is Effectively Modulated by Curcumin and Emetine Conjugated PLGA Nanoparticles

et al. 2015

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Small molecule based therapeutic intervention of amyloids has been limited by their low solubility and poor pharmacokinetic characteristics. We report here, the use of water soluble poly lactic-co-glycolic acid (PLGA)-encapsulated curcumin and emetine nanoparticles (Cm-NPs and Em-NPs, respectively), as potential modulators of gelsolin amyloidogenesis. Using the amyloid-specific dye Thioflavin T (ThT) as an indicator along with electron microscopic imaging we show that the presence of Cm-NPs augmented amyloid formation in gelsolin by skipping the pre-fibrillar assemblies, while Em-NPs induced non-fibrillar aggregates. These two types of aggregates differed in their morphologies, surface hydrophobicity and secondary structural signatures, confirming that they followed distinct pathways. In spite of differences, both these aggregates displayed reduced toxicity against SH-SY5Y human neuroblastoma cells as compared to control gelsolin amyloids. We conclude that the cytotoxicity of gelsolin amyloids can be reduced by either stalling or accelerating its fibrillation process. In addition, Cm-NPs increased the fibrillar bulk while Em-NPs defibrillated the pre-formed gelsolin amyloids. Moreover, amyloid modulation happened at a much lower concentration and at a faster rate by the PLGA encapsulated compounds as compared to their free forms. Thus, besides improving pharmacokinetic and biocompatible properties of curcumin and emetine, PLGA conjugation elevates the therapeutic potential of both small molecules against amyloid fibrillation and toxicity.

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Differential permeation of piroxicam-loaded PLGA micro/nanoparticles and their in vitro enhancement

Cited by 7 publications

References 48 publications

Dual delivery nanosystem for biomolecules. Formulation, characterization, and in vitro release

Dual delivery nanosystem for biomolecules. Formulation, characterization, and in vitro release

Ex vivo permeation of tamoxifen and its 4-OH metabolite through rat intestine from lecithin/chitosan nanoparticles

Gelsolin Amyloidogenesis Is Effectively Modulated by Curcumin and Emetine Conjugated PLGA Nanoparticles

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