Differential proteome analysis of serum proteins associated with the development of type 2 diabetes mellitus in the KK-Ay mouse model using the iTRAQ technique

Takahashi, Eri; Okumura, Akinori; Unoki-Kubota, Hiroyuki; Hirano, Hisashi; Kasuga, Masato; Kaburagi, Yasushi

doi:10.1016/j.jprot.2013.03.014

Cited by 23 publications

(24 citation statements)

References 35 publications

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“…It is not immediately clear why extracellular proteins would localize intracellularly to LDs or why their localization would be affected by hepatic steatosis; however, many of the identifi ed extracellular proteins have links to hepatic steatosis, obesity, and diabetes (72)(73)(74)(75)(76). Additionally, it is not clear whether these extracellular proteins are in the secretory pathway or are being endocytosed.…”

Section: Discussionmentioning

confidence: 99%

Quantitative analysis of the murine lipid droplet-associated proteome during diet-induced hepatic steatosis

Khan

Wollaston‐Hayden

Markowski

et al. 2015

Journal of Lipid Research

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Section: Discussionmentioning

confidence: 99%

Quantitative analysis of the murine lipid droplet-associated proteome during diet-induced hepatic steatosis

Khan

Wollaston‐Hayden

Markowski

et al. 2015

Journal of Lipid Research

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“…Accumulating evidence indicates protein profiling of blood, serum and plasma varies between healthy individuals and those with insulin resistance or type 2 diabetes42. Earlier proteomic research on a type 2 diabetes mouse model showed serine protease inhibitor A3K (SERPINA3K) levels were elevated in the serum of mice compared with those in normal mice43. SERPINA3K is suggested to inhibit proteolytic activities of tissue kallikrein and potentially participate in the regulation of vasodilation and local blood flow44.…”

Section: Discussionmentioning

confidence: 99%

Perfluorooctanoic acid exposure for 28 days affects glucose homeostasis and induces insulin hypersensitivity in mice

Yan

Zhang

Zheng

et al. 2015

Sci Rep

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Perfluoroalkyl acids (PFAAs) are widely used in many applications due to their unique physical and chemical characteristics. Because of the increasing prevalence of metabolic syndromes, including obesity, dyslipidemia and insulin resistance, concern has arisen about the roles of environmental pollutants in such diseases. Earlier epidemiologic studies showed a potential association between perfluorooctanoic acid (PFOA) and glucose metabolism, but how PFOA influences glucose homeostasis is still unknown. Here, we report on the modulation of the phosphatidylinositol 3-kinase-serine/threonine protein kinase (PI3K-AKT) signaling pathway in the livers of mice after 28 d of exposure to PFOA. Compared with normal mice, PFOA exposure significantly decreased the expression of the phosphatase and tensin homologue (PTEN) protein and affected the PI3K-AKT signaling pathway in the liver. Tolerance tests further indicated that PFOA exposure induced higher insulin sensitivity and glucose tolerance in mice. Biochemical analysis revealed that PFOA exposure reduced hepatic glycogen synthesis, which might be attributed to gluconeogenesis inhibition. The levels of several circulating proteins were altered after PFOA exposure, including proteins potentially related to diabetes and liver disease. Our results suggest that PFOA affected glucose metabolism and induced insulin hypersensitivity in mice.

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“…They grouped and described the validated markers as a diet associated panel of apolipoproteins (ie, APOE, APO B‐100, APOA‐IV and APOC‐III), T2D associated proteins (Fibrinogen alpha, beta and gamma chain and transthyretin) and obesity associated (complement 3). Other studies of T2D include the use of a mouse model and plasma protein carbonylation in rats …”

Section: Applications Of Isobaric Labeling For Serum and Plasma Protementioning

confidence: 99%

Analysis of the plasma proteome using iTRAQ and TMT‐based Isobaric labeling

Moulder

Bhosale

Goodlett

et al. 2017

Mass Spectrometry Reviews

129

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Over the past decade, chemical labeling with isobaric tandem mass tags, such as isobaric tags for relative and absolute quantification reagents (iTRAQ) and tandem mass tag (TMT) reagents, has been employed in a wide range of different clinically orientated serum and plasma proteomics studies. In this review the scope of these works is presented with attention to the areas of research, methods employed and performance limitations. These applications have covered a wide range of diseases, disorders and infections, and have implemented a variety of different preparative and mass spectrometric approaches. In contrast to earlier works, which struggled to quantify more than a few hundred proteins, increasingly these studies have provided deeper insight into the plasma proteome extending the numbers of quantified proteins to over a thousand.

show abstract

Differential proteome analysis of serum proteins associated with the development of type 2 diabetes mellitus in the KK-Ay mouse model using the iTRAQ technique

Cited by 23 publications

References 35 publications

Quantitative analysis of the murine lipid droplet-associated proteome during diet-induced hepatic steatosis

Quantitative analysis of the murine lipid droplet-associated proteome during diet-induced hepatic steatosis

Perfluorooctanoic acid exposure for 28 days affects glucose homeostasis and induces insulin hypersensitivity in mice

Analysis of the plasma proteome using iTRAQ and TMT‐based Isobaric labeling

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