2010
DOI: 10.1186/1475-2859-9-95
|View full text |Cite
|
Sign up to set email alerts
|

Differential proteomic analysis highlights metabolic strategies associated with balhimycin production in Amycolatopsis balhimycina chemostat cultivations

Abstract: BackgroundProteomics was recently used to reveal enzymes whose expression is associated with the production of the glycopeptide antibiotic balhimycin in Amycolatopsis balhimycina batch cultivations. Combining chemostat fermentation technology, where cells proliferate with constant parameters in a highly reproducible steady-state, and differential proteomics, the relationships between physiological status and metabolic pathways during antibiotic producing and non-producing conditions could be highlighted.Result… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
4
1

Citation Types

1
19
0

Year Published

2012
2012
2017
2017

Publication Types

Select...
4
3

Relationship

1
6

Authors

Journals

citations
Cited by 18 publications
(20 citation statements)
references
References 50 publications
(87 reference statements)
1
19
0
Order By: Relevance
“…A recent example of this technology has involved the use of the differential proteomic approach which has shown that the production of the antibiotic balhimycin in batch culture is associated with the up-regulation of enzymes involved in the biosynthesis of antibiotic precursors. These proteomic data point to the possible sustainable balhimycin production through availability of increased levels of precursors, such as tyrosine, that has also proved to be effective in balhimycin production (Gallo et al, 2010).…”
Section: Amycolatopsis Balhimycinamentioning
confidence: 81%
See 3 more Smart Citations
“…A recent example of this technology has involved the use of the differential proteomic approach which has shown that the production of the antibiotic balhimycin in batch culture is associated with the up-regulation of enzymes involved in the biosynthesis of antibiotic precursors. These proteomic data point to the possible sustainable balhimycin production through availability of increased levels of precursors, such as tyrosine, that has also proved to be effective in balhimycin production (Gallo et al, 2010).…”
Section: Amycolatopsis Balhimycinamentioning
confidence: 81%
“…The minimal defined media consisted of low Pi concentrations and proficient glucose, or high Pi levels and limiting glucose concentrations. The biomass obtained from these cultivations were then analyzed by a comparative proteomic study in order to reveal the expression of genes that are involved in A. balhimycina primary and secondary metabolism which are associated with biomass production and antibiotic synthesis (Gallo et al, 2010). The medium containing low Pi levels produced balhimycin.…”
Section: Amycolatopsis Balhimycinamentioning
confidence: 99%
See 2 more Smart Citations
“…Total proteins were extracted from frozen biomass samples of quadruplicated cultivations per each condition by using an experimental procedure previously described [69,70]. Briefly, pellets were cleaned three times with a washing solution (10 mM Tris–HCl pH 7.5, 5 mM EDTA, 1 mM DTT, 0.5 mM PMSF, 4 mg/ml leucopeptin, 0.7 mg/ml pepstatin, 5 mg/ml benzamidin).…”
Section: Methodsmentioning
confidence: 99%