Differential proteomics analysis to identify proteins and pathways associated with male sterility of soybean using iTRAQ-based strategy

Li, Jiajia; Ding, Xianlong; Han, Sang‐Mi; He, Tingting; Zhang, Hao; Yang, Longshu; Yang, Shouping; Gai, Junyi

doi:10.1016/j.jprot.2016.02.017

Cited by 57 publications

(49 citation statements)

References 68 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…iTRAQ labeling and SCX fractionation, LC‐ESI‐MS/MS analysis based on Q EXACTIVE, and data analysis were performed according to the iTRAQ manufacturer's instructions (Chen et al., ; Li et al., ; Liu et al., ; Qiao et al., , ; Tian et al., ).…”

Section: Methodsmentioning

confidence: 99%

Comparative proteomic analysis of Methanothermobacter thermautotrophicus reveals methane formation from H₂ and CO₂ under different temperature conditions

et al. 2018

View full text Add to dashboard Cite

The growth of all methanogens is limited to a specific temperature range. However, Methanothermobacter thermautotrophicus can be found in a variety of natural and artificial environments, the temperatures of which sometimes even exceed the temperature growth ranges of thermophiles. As a result, the extent to which methane production and survival are affected by temperature remains unclear. To investigate the mechanisms of methanogenesis that Archaea have evolved to cope with drastic temperature shifts, the responses of Methanothermobacter thermautotrophicus to temperature were investigated under a high temperature growth (71°C) and cold shock (4°C) using Isobaric tags for relative and absolute quantitation (iTRAQ). The results showed that methane formation is decreased and that protein folding and degradation are increased in both high‐ and low‐temperature treatments. In addition, proteins predicted to be involved in processing environmental information processing and in cell membrane/wall/envelope biogenesis may play key roles in affecting methane formation and enhancing the response of M. thermautotrophicus to temperature stress. Analysis of the genomic locations of the genes corresponding to these temperature‐dependent proteins predicted that 77 of the genes likely to form 32 gene clusters. Here, we assess the response of M. thermautotrophicus to different temperatures and provide a new level of understanding of methane formation and cellular putative adaptive responses.

show abstract

Section: Methodsmentioning

confidence: 99%

Comparative proteomic analysis of Methanothermobacter thermautotrophicus reveals methane formation from H₂ and CO₂ under different temperature conditions

et al. 2018

View full text Add to dashboard Cite

show abstract

“…However, some discrepancies have been found between transcript content analysis and protein content analysis. A more accurate method involves iTRAQ‐based technology that can simultaneously label and accurately quantify proteins from multiple samples, and it has been used to quantify and characterise changes in protein levels in a wide range of studies . In this experiment, the protein changes accompanying ‘Nanguo’ pear fruit PB were analysed using iTRAQ.…”

Section: Introductionmentioning

confidence: 99%

Proteomic analysis of peel browning of ‘Nanguo’ pears after low‐temperature storage

Wang

Zhou

et al. 2016

J Sci Food Agric

View full text Add to dashboard Cite

show abstract

“…In the present study, the distribution proportion of proteins determined using iTRAQ after functional classification which were identified at the floral bud and florescence stages of tomato, exhibited similar proteomic profiles as other dicotyledons (including tomato, Arabidopsis , rapeseed, and soybean). [ 16,42–48 ] We found that the majority of DAPs were detected between the 2–517 and VF‐11 lines at the floral bud stage (Figure 2A), and this implies that this stage is central for pollen development. Furthermore, comparison of the proteins which were differentially expressed at the floral bud stage in the 2–517 and VF‐11 lines, found that the DAPs were mainly involved in oxidoreductase activity, the inositol biosynthetic process, fatty acid oxidation, and the metabolic process of carbohydrate and lipid oxidation(Figure 4A); whereas at the florescence stage, the DAPs were associated with oxidoreductase activity, and the metabolic process of monocarboxylic acid, carboxylic acid, small molecules, and oxoacid.…”

Section: Discussionmentioning

confidence: 81%

iTRAQ‐Based Proteomics Reveals that the Tomato ms10³⁵ Gene Causes Male Sterility through Compromising Fat Acid Metabolism

Wang

et al. 2020

Proteomics

View full text Add to dashboard Cite

So far, over 50 spontaneous male sterile mutants of tomato have been described and most of them are categorized as genetic male sterility. To date, the mechanism of tomato genetic male sterility remained unclear. In this study, differential proteomic analysis is performed between genetic male sterile line (2-517), which carries the male sterility (ms10 35 ) gene, and its wildtype (VF-11) using isobaric tags for relative and absolute quantification-based strategy. A total of 8272 proteins are quantified in the 2-517 and VF-11 lines at the floral bud and florescence stages. These proteins are involved in different cellular and metabolic processes, which express obvious functional tendencies toward the hydroxylation of the -carbon in fatty acids, the tricarboxylic acid cycle, the glycolytic, and pentose phosphate pathways. Based on the results, a protein network explaining the mechanisms of tomato genetic male sterility is proposed, finding the compromising fat acid metabolism may cause the male sterility. These results are confirmed by parallel reaction monitoring, quantitative Real-time PCR (qRT-PCR), and physiological assays. Taken together, these results provide new insights into the metabolic pathway of anther abortion induced by ms10 35 and offer useful clues to identify the crucial proteins involved in genetic male sterility in tomato.

show abstract

Differential proteomics analysis to identify proteins and pathways associated with male sterility of soybean using iTRAQ-based strategy

Cited by 57 publications

References 68 publications

Comparative proteomic analysis of Methanothermobacter thermautotrophicus reveals methane formation from H₂ and CO₂ under different temperature conditions

Comparative proteomic analysis of Methanothermobacter thermautotrophicus reveals methane formation from H₂ and CO₂ under different temperature conditions

Proteomic analysis of peel browning of ‘Nanguo’ pears after low‐temperature storage

iTRAQ‐Based Proteomics Reveals that the Tomato ms10³⁵ Gene Causes Male Sterility through Compromising Fat Acid Metabolism

Contact Info

Product

Resources

About

Differential proteomics analysis to identify proteins and pathways associated with male sterility of soybean using iTRAQ-based strategy

Cited by 57 publications

References 68 publications

Comparative proteomic analysis of Methanothermobacter thermautotrophicus reveals methane formation from H2 and CO2 under different temperature conditions

Comparative proteomic analysis of Methanothermobacter thermautotrophicus reveals methane formation from H2 and CO2 under different temperature conditions

Proteomic analysis of peel browning of ‘Nanguo’ pears after low‐temperature storage

iTRAQ‐Based Proteomics Reveals that the Tomato ms1035 Gene Causes Male Sterility through Compromising Fat Acid Metabolism

Contact Info

Product

Resources

About

Comparative proteomic analysis of Methanothermobacter thermautotrophicus reveals methane formation from H₂ and CO₂ under different temperature conditions

Comparative proteomic analysis of Methanothermobacter thermautotrophicus reveals methane formation from H₂ and CO₂ under different temperature conditions

iTRAQ‐Based Proteomics Reveals that the Tomato ms10³⁵ Gene Causes Male Sterility through Compromising Fat Acid Metabolism