Differential radioactive quantification of protein abundance ratios between benign and malignant prostate tissues: Cancer association of annexin A3

Abstract

A differential quantitative protein expression study, comparing matched prostate cancerous and benign tissues from 31 patients, revealed proteins newly associated with prostate cancer. Average effects for 17 proteins whose abundance was significantly different (p<0.01) across patients ranged from 1.5- to 6.1-fold, and included a number of known cancer markers. The most differentially abundant proteins between cancer and benign samples were isopeptidase T, serum amyloid P (SAP), annexin A3 (ANXA3) and mitochondrial enoyl coenzyme-A hydratase. SAP is restricted to stroma in healthy tissue, and the lower abundance in tumours may be explained by the reduced stromal content. ANXA3 is present in healthy epithelial cells, exhibits strong staining in precancerous prostatic intraepithelial neoplasia, and is relatively less abundant in individual tumour cells of increasing Gleason pattern (GP), despite exhibiting higher overall tissue abundance in tumours. ANXA3 staining was predominantly cytoplasmic, yet nuclear localization was also observed. Strongly staining single cells, possibly phagocytes, were interspersed in highly dedifferentiated GP5 tumour areas among tumour cells without measurable ANXA3. Local recurrent androgen ablation therapy-resistant tumours exhibit heterogenous low levels of ANXA3 staining. Results are discussed focussing on the potential implications for tumour tissues.

Increased Expression of Annexin A3 Is a Mechanism of Platinum Resistance in Ovarian Cancer

“…However, annexins A1, A2, A7, and A11 were reported to be downregulated in a number of tumors (32,33). In particular, expression of annexin A3 is increased in colorectal cancer (34) and prostate cancer (35). It was also found that colorectal cancer had increased expression of multiple annexins, including A1, A2, A4, and A11 (36).…”

Primary Cell Cultures from Human Renal Cortex and Renal-Cell Carcinoma Evidence a Differential Expression of Two Spliced Isoforms of Annexin A3

“…Otherwise, AnxA3 has been found to be down-regulated also in prostate cancer, in which a heterogeneous expression has been described by TMA analysis. 40 The down-regulation of AnxA3 in prostate cancer has been suggested to occur in a context of autoimmunity that, however, cannot be invoked in our in vitro model. In our study, two protein bands of AnxA3 were detected in primary cell culture lysates of both normal cortex and RCC.…”

Characterisation and protein expression profiling of annexins in colorectal cancer

“…Furthermore, while there has been a range of studies of individual annexin expression in specific types of primary tumours, there have been no significant previous studies of annexin expression in metastasis. In addition, more recently individual annexins have been proposed as putative tumour biomarkers and potential therapeutic targets in cancer (Oh et al, 2004;Falsey et al, 2006;Wozny et al, 2007). The expression and cellular localisation of annexins have received very little previous study in colorectal cancer, with only annexin A2 having been investigated (Emoto et al, 2001).…”

“…The overall expression profile of annexins was associated with survival in colorectal cancer, indicating collectively that annexin expression may contribute to outcome and this would be consistent with the putative roles of annexins in some of the cellular processes that led to tumour invasion. These annexins may also represent tumour biomarkers and potential therapeutic targets (Oh et al, 2004;Falsey et al, 2006;Wozny et al, 2007).…”