Differential radioprotection of bone marrow and tumour cells by zinc aspartate

Floersheim, G. L.; Chiodetti, N; Bieri, A.

doi:10.1259/0007-1285-61-726-501

Cited by 31 publications

(16 citation statements)

References 12 publications

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“…Interesting, the efficacy of genistein on enhancement of the survival and promoting recovery of leukocytes, lymphocytes and bone marrow nucleated cells were stronger than those of DES in our experiments, although its protection against the decrease of thrombocyte counts was weaker than that of DES. According to the conclusions of Floersheim et al, 20) it would appear that genistein affords hematological protection by both preventing the destruction blood cells and enhancing hematopoietic recovery. That means not only that the circulating blood cells but also the progenitor cells may be protected under irradiation by prior genistein administration.…”

Section: Discussionmentioning

confidence: 99%

Genistein Stimulates Hematopoiesis and Increases Survival in Irradiated Mice

Zhou

2005

JRR

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Radiation protection from death and stimulating hematopoietic recovery by oral administrations of genistein, 160 mg/kg b.w., once daily for seven consecutive days before whole-body gamma-rays irradiation, were confirmed by tests with adult male BALB/c mice. Moreover, the protective action of genistein was compared to that of diethylstilbestrol (DES). Based on the studies of survival, behavior of hematograms, endogenous hematopoietic spleen colony formation (endoCFUs), and numbers of nucleated cell, granulocyte-macrophage colony forming units (CFU-GM) in bone marrow following irradiation, it was demonstrated that genistein was an effective radioprotector. The survival of irradiated mice protected by genistein was significantly increased and statistically higher than that of mice pre-treated with DES. Stimulated recovery of leukocytes, erythrocytes, lymphocytes and thrombocytes were observed in mice pre-treated with genistein or DES, however, the effects of genistein on promoting recovery of bone marrow nucleated cells, leukocytes and lymphocytes were significantly higher than those of DES. Enhanced endoCFUs, numbers of bone marrow nucleated cells and CFU-GM were also found in mice pre-treated with genistein as well as DES. Meanwhile, endoCFU numbers in mice pre-treated with genistein was 3.47-fold higher than that in the irradiated control group, although no significant difference was found between genistein administration and DES administration. It could be deduced that the radioprotective action against death is induced by a possible process of enhanced regeneration of the hematopoietic stem cells due to not only strengthened radioresistance and increased numbers of remained hematopoietic cells, but also enhanced post-irradiation repair or promoted proliferation of the hematopoietic stem cells. These effects of genistein may have some therapeutic implications for radiation-induced injuries.

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Section: Discussionmentioning

confidence: 99%

Genistein Stimulates Hematopoiesis and Increases Survival in Irradiated Mice

Zhou

2005

JRR

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“…The in vivo radioprotective test was performed using non-tumor bearing Balb/c mice (Floersheim et al, 1988). Blood samples were taken from the retroorbital plexus, and blood cells were counted with a Coulter counter.…”

Section: Blood Counts and Survival Ratementioning

confidence: 99%

Protective effects of fucoidan against γ-radiation-induced damage of blood cells

Rhee

Lee

2011

Arch. Pharm. Res.

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Fucoidan, a sulfated polysaccharide purified from brown algae including Fucus vesiculosus and Laminaria japonica, has a variety of biological activities, including antioxidant and antitumor activities. Here, we investigated the radioprotective effects of fucoidan on human monoblastic leukemia cell line U937. Further, animal tests were carried out using Balb/c mice in order to determine the radiation-induced changes in the counts of blood cells, including thrombocytes, erythrocytes, leukocytes and hematocrit. Cell viability was assessed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, wherein fucoidan (1, 10, and 100 μg/mL) was observed to improve recovery from damage caused by 8-Gy radiation in a dose dependent manner. The viability of U937 cells pre-treated with fucoidan also increased in a dose dependent manner. Furthermore, fucoidan at 100 mg/kg was found to protect against changes in the counts of blood cells as follows: on day 28 after irradiation, the thrombocyte count in the irradiated controls decreased to 45% compared with the non-irradiated controls, while that in the fucoidan-treated group was 60%. The hematocrit in the fucoidan-treated group recovered to 75% on day 28, while that in the irradiated control was 68%. The erythrocyte count in the irradiated controls consistently ranged from 64% to 67% throughout the experiment, but that in the fucoidan-treated group increased gradually, ranging from 75% to 80%. The mean number of survival days and 50-day actuarial survival rate increased dose dependently in the fucoidan-treated group. The mean number of survival days and the 50-day actuarial survival rate in this group was 16, 21, and 29 days and 12%, 20%, and 30% at fucoidan doses of 1, 10, and 100 mg/kg. The values of these parameters in the control group were 9 days and 0%, although the difference between the test and control groups was not statistically significant. Our results may prove valuable in the field of radioprotection.

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“…Zinc is ubiquitous in sub-cellular metabolism and an essential component of catalytic site or sites of at least one enzyme in every enzyme classification [15,16]. Zinc salts may be considered as a new class of radio protectors and has been shown to provide radioprotection against whole body irradiation [17][18][19]. Moreover, a number of studies have suggested zinc as a beneficial agent during peroxidative damage [20,21].…”

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confidence: 99%

131I Induced Hematological Alterations in Rat Blood: Protection by Zinc

Dani

Malhotra

Dhawan

2007

Biol Trace Elem Res

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The present study was planned to determine the potential of zinc in attenuating the toxicity induced by 131I in rat blood. Female wistar rats were segregated into four main groups. Animals in Group I served as normal controls; Group II animals were administered a dose of 3.7 Mbq of 131I (carrier free) intraperitoneally, Group III was supplemented with Zinc in the form of ZnSo4.7H2O (227 mg/l drinking water), and Group IV was given a combined treatment of Zinc as well as 131I, in a similar way as was given to Groups IV and II animals, respectively. The effects of different treatments were studied on various parameters in rat blood including hemoglobin (Hb) levels, % hematocrit, zinc protoporphyrins (ZPP), activities of enzymes which included aminolevulinic acid dehydratase (delta-ALAD) and Na+ K+ ATPase and uptake of 65Zn in blood. The study revealed an increase in the levels of hemoglobin, % hematocrit, activities of delta-ALAD, Na+ K+ ATPase and uptake of 65Zn, 7 days after the 131I treatment. On the contrary, the levels of ZPP were found to be significantly decreased after 131I treatment. However, zinc treatment to 131I-treated animals significantly attenuated the various biochemical and hematological indices. Moreover, zinc treatment to the 131I-treated animals could significantly decrease the uptake of 65Zn, which was increased after 131I treatment. Based upon these data, the present study suggests that zinc has the potential to attenuate 131I induced toxicity by restoring the altered hematological indices and biochemical changes.

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Differential radioprotection of bone marrow and tumour cells by zinc aspartate

Cited by 31 publications

References 12 publications

Genistein Stimulates Hematopoiesis and Increases Survival in Irradiated Mice

Genistein Stimulates Hematopoiesis and Increases Survival in Irradiated Mice

Protective effects of fucoidan against γ-radiation-induced damage of blood cells

131I Induced Hematological Alterations in Rat Blood: Protection by Zinc

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