2012
DOI: 10.1111/jre.12028
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Differential regulation of collagen, lysyl oxidase and MMP‐2 in human periodontal ligament cells by low‐ and high‐level mechanical stretching

Abstract: This study substantiates the mechanoregulation of the expression of ECM-related molecules in PDL cells. High-level mechanical stretching upregulated the expression of MMP2 and TIMP2 mRNAs, but did not affect collagen production or LOX activity. In addition to increasing the transcription of COL1A1, COL3A1 and LOX genes, low-level mechanical stretching enhanced total collagen production and LOX activity, which should favor ECM stabilization. As an effective regulator of ECM remodeling, mechanical stretching can… Show more

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Cited by 34 publications
(31 citation statements)
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“…This indicates that the culture environment could also influence on cellular response. To analyze the effect of mechanical loading on PDL-derived cells in vitro , a number of investigators have used commercially available loading apparatus, such as Flexcell (Flexcell International Co., Hillsborough, NC) [14-19], Strex (STREX Inc., Okayama, Japan) [20,21] and general laboratory centrifuge [17,22,23], while others fabricated their own loading devices [24-27]. Since optimal mechanical loading varies depending on cell type, culture condition and loading mode, it is important to use well defined loading regimen with a thoroughly characterized loading apparatus.…”
Section: Mechanical Loading In Pdlmentioning
confidence: 99%
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“…This indicates that the culture environment could also influence on cellular response. To analyze the effect of mechanical loading on PDL-derived cells in vitro , a number of investigators have used commercially available loading apparatus, such as Flexcell (Flexcell International Co., Hillsborough, NC) [14-19], Strex (STREX Inc., Okayama, Japan) [20,21] and general laboratory centrifuge [17,22,23], while others fabricated their own loading devices [24-27]. Since optimal mechanical loading varies depending on cell type, culture condition and loading mode, it is important to use well defined loading regimen with a thoroughly characterized loading apparatus.…”
Section: Mechanical Loading In Pdlmentioning
confidence: 99%
“…Comparative studies have been performed in order to analyze the effects of different loading conditions on the gene expression of type I collagen in PDL. A recent study by Chen et al showed that 3% cyclic stretching increased the gene expression of COL1A1 but decreased by 10% cyclic stretching on human PDL-derived cells [19]. Another study, by He et al compared the effect of cyclic equibiaxial tension and compressive forces on the expression of type I collagen by using human PDL-derived cells [24].…”
Section: Type I Collagenmentioning
confidence: 99%
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“…For each cell culture, the levels of mRNA expressed for 25 UPR‐related genes and three mechanical strain‐responsive genes (Table S1) were assessed using a custom TaqMan 96‐well array (3 × 32 gene layout; Life Technologies). The mechanical strain‐responsive genes were selected based on their use in previous monolayer and 3D culture studies, while the UPR‐related genes that were selected ensured representation of each of the three arms of the UPR process . The custom array included three reference genes [glyceraldehyde‐3‐phosphate dehydrogenase ( GAPDH ), beta‐2 microglobulin ( B2M ), and 60S ribosomal protein L13a ( RPL13A )].…”
Section: Methodsmentioning
confidence: 99%