Differential Regulation of Inflammatory Cytokine Secretion by Human Dendritic Cells upon
            <i>Chlamydia trachomatis</i>
            Infection

Gervassi, Ana; Alderson, Mark R.; Suchland, Robert J.; Maisonneuve, Jeff; Grabstein, Kenneth H.; Probst, Peter

doi:10.1128/iai.72.12.7231-7239.2004

Cited by 72 publications

(78 citation statements)

References 38 publications

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“…Caspase-1 itself must be activated by autoprocessing in the cytoplasm of the cell. This occurs in phagocytes in response to many intracellular bacteria, including Francisella (26,60,69). In essence, the production of active IL-1␤ requires at least two signaling events: the transcriptional upregulation and synthesis of pro-IL-1␤ and the activation of caspase-1 in order to process pro-IL-1␤.…”

Section: Discussionmentioning

confidence: 99%

Host Immune Response and Acute Disease in a Zebrafish Model ofFrancisellaPathogenesis

et al. 2009

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Members of the bacterial genus Francisella are highly virulent and infectious pathogens. New models to study Francisella pathogenesis in evolutionarily distinct species are needed to provide comparative insight, as the mechanisms of host resistance and pathogen virulence are not well understood. We took advantage of the recent discovery of a novel species of Francisella to establish a zebrafish/Francisella comparative model of pathogenesis and host immune response. Adult zebrafish were susceptible to acute Francisella-induced disease and suffered mortality in a dose-dependent manner. Using immunohistochemical analysis, we localized bacterial antigens primarily to lymphoid tissues and livers of zebrafish following infection by intraperitoneal injection, which corresponded to regions of local cellular necrosis. Francisella sp. bacteria replicated rapidly in these tissues beginning 12 h postinfection, and bacterial titers rose steadily, leveled off, and then decreased by 7 days postinfection. Zebrafish mounted a significant tissue-specific proinflammatory response to infection as measured by the upregulation of interleukin-1␤ (IL-1␤), gamma interferon, and tumor necrosis factor alpha mRNA beginning by 6 h postinfection and persisting for up to 7 days postinfection. In addition, exposure of zebrafish to heat-killed bacteria demonstrated that the significant induction of IL-1␤ was highly specific to live bacteria. Taken together, the pathology and immune response to acute Francisella infection in zebrafish share many features with those in mammals, highlighting the usefulness of this new model system for addressing both general and specific questions about Francisella host-pathogen interactions via an evolutionary approach.

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Section: Discussionmentioning

confidence: 99%

Host Immune Response and Acute Disease in a Zebrafish Model ofFrancisellaPathogenesis

et al. 2009

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“…ROS production also leads to activation of caspase 1 through the NLRP3-ASC inflammasome and processing of the proinflammatory cytokines IL-1b and IL-18 in Chlamydia-infected cells (Rothermel et al 1989;Entrican et al 1999;Lu et al 2000;Gervassi et al 2004;Abdul-Sater et al 2009;He et al 2010). …”

Section: Chlamydial Intracellular Survival Strategiesmentioning

confidence: 99%

Chlamydial Intracellular Survival Strategies

Bastidas

Elwell

Engel

et al. 2013

Cold Spring Harbor Perspectives in Medicine

206

197

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Chlamydia trachomatis is the most common sexually transmitted bacterial pathogen and the causative agent of blinding trachoma. Although Chlamydia is protected from humoral immune responses by residing within remodeled intracellular vacuoles, it still must contend with multilayered intracellular innate immune defenses deployed by its host while scavenging for nutrients. Here we provide an overview of Chlamydia biology and highlight recent findings detailing how this vacuole-bound pathogen manipulates host -cellular functions to invade host cells and maintain a replicative niche.

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“…To avoid potential CDT toxicity on DC, Xu and colleagues studied interactions between myeloid DC and gentamicinkilled H. ducreyi bacteria (47). However, interactions between DC and dead bacteria do not always reflect those found between DC and live bacteria (7,10,29,49). To our knowledge, no studies have examined how myeloid DC interact with live H. ducreyi.…”

mentioning

confidence: 99%

“…H. ducreyi was grown to mid-log phase in brain heart infusion broth and washed three times with Hanks balanced salt solution. Live bacteria were centrifuged onto wells containing approximately 10 5 DC at a multiplicity of infection (MOI) of approximately 20:1 and incubated for 90 min at 35°C in 5% CO 2 . DC were collected by centrifugation, and nonadherent bacteria were removed by washing with RPMI 1640.…”

Section: Characterization Of DC From Biopsy Samples Of H Ducreyi-infmentioning

confidence: 99%

Haemophilus ducreyi Partially Activates Human Myeloid Dendritic Cells

Banks¹,

Humphreys²,

Li³

et al. 2007

Infect Immun

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Differential Regulation of Inflammatory Cytokine Secretion by Human Dendritic Cells upon Chlamydia trachomatis Infection

Cited by 72 publications

References 38 publications

Host Immune Response and Acute Disease in a Zebrafish Model ofFrancisellaPathogenesis

Host Immune Response and Acute Disease in a Zebrafish Model ofFrancisellaPathogenesis

Chlamydial Intracellular Survival Strategies

Haemophilus ducreyi Partially Activates Human Myeloid Dendritic Cells

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