Differential requirements for baculovirus late expression factor genes in two cell lines

Lu, Albert; Miller, Lois K.

doi:10.1128/jvi.69.10.6265-6272.1995

Cited by 87 publications

(45 citation statements)

References 65 publications

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“…HRF-1 (host range factor 1) and HCF-1, which are encoded by LdMNPV and AcMNPV, respectively (Lu & Miller 1995;Thiem et al 1996), preclude global protein synthesis shutdown in a cell-line specific manner, thereby restoring the production of progeny viruses. Ld652Y cells are non-permissive for AcMNPV productive infection and induce global protein synthesis shutdown upon infection with AcMNPV (Thiem et al 1996).…”

Section: Baculovirus Factors That Expand Host Range: Hrf-1 and Hcf-1mentioning

confidence: 99%

Baculoviruses: diversity, evolution and manipulation of insects

Ikeda

Hamajima

Kobayashi

2014

Entomological Science

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Baculoviruses, members of the family Baculoviridae, are large, enveloped viruses that contain a doublestranded circular DNA genome of 80-180 kbp, encoding 90-180 putative proteins. These viruses are exclusively pathogenic for arthropods, particularly insects, and have been developed, or are being developed, as environmentally sound pesticides and eukaryotic vectors for foreign protein expression, surface display, gene delivery for gene therapy, vaccine production and drug screening. The baculoviruses contain a set of approximately 30 core genes that are conserved among all baculovirus genomes sequenced to date. Individual baculoviruses also contain a number of lineage-or species-specific genes that have greatly impacted the diversification and evolution of baculoviruses. In this review, we first describe the general properties and biology of baculoviruses and then focus on the baculovirus genes and mechanisms involved in the replication, spread and survival of baculoviruses within the context of their diversity, evolution and insect manipulation.

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Section: Baculovirus Factors That Expand Host Range: Hrf-1 and Hcf-1mentioning

confidence: 99%

Baculoviruses: diversity, evolution and manipulation of insects

Ikeda

Hamajima

Kobayashi

2014

Entomological Science

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“…Other ODV capsid-associated proteins with potential involvement in DNA replication include host cell factor HCF-1 (Braunagel et al, 2003) and viral proliferating cell nuclear antigen (PCNA) (O'Reilly et al, 1989). HCF-1 is required for AcMNPV DNA replication in Trichoplusia ni cell lines (Lu and Miller, 1995a) and it augments the ability of this virus to orally infect T. ni larvae (Lu and Miller, 1996).…”

Section: Dna Replication Proteins In the Nucleocapsidmentioning

confidence: 99%

The Baculoviruses Occlusion‐Derived Virus: Virion Structure and Function

Slack

Arif

2006

Advances in Virus Research

264

240

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“…Two other AcMNPV genes, p35 and lef-7, were previously shown to activate transient expression of a late reporter gene in SF-21 cells but have little or no effect in equivalent transient expression assays in TN-368 cells (22). Mutant viruses defective in p35 or lef-7 have also been successfully isolated and propagated in T. ni cells (6,7).…”

Section: Discussionmentioning

confidence: 99%

“…To examine the role of the AcMNPV ie-2 gene during viral infection, we constructed ie-2 mutant viruses with deletions of different functional regions of IE-2. Since ie-2 is not essential for transient trans activation of the late vp39 promoter in TN-368 cells (22), all ie-2 mutant viruses were constructed by using a cell line derived from T. ni cells. The first of these recombinant viruses, vie2Z, contained an insertion of the E. coli lacZ gene within the ie-2 coding sequences ( Fig.…”

Section: Construction Of Ie-2 Mutant Virusesmentioning

confidence: 99%

“…It is possible that the role of IE-2 in these assays is simply to stimulate ie-1 expression or expression from other early genes required for plasmid replication or stability. The stimulatory effect of IE-2 on hr-containing plasmid DNA replication or stability and coupled late gene expression is observed in SF-21 cells, a cell line derived from the fall armyworm S. frugiperda, but not in TN-368 cells or in TN-5B1-4 cells (22), derived from the cabbage looper T. ni. Mutants of AcMNPV defective in ie-2 have not been previously described, but the cell line-specific effects of ie-2 on DNA replication and late gene expression prompted us to attempt to construct viruses with ie-2 mutations in order to define the function of IE-2 and its possible role in host range.…”

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confidence: 99%

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In Vivo and In Vitro Analysis of Baculovirus ie-2 Mutants

Prikhod’ko

Wilson

et al. 1999

J Virol

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Upon transient expression in cell culture, the ie-2 gene of Autographa californica nuclear polyhedrosis virus (AcMNPV) displays three functions: trans activation of viral promoters, direct or indirect stimulation of virus origin-specific DNA replication, and arrest of the cell cycle. The ability of IE2 to trans stimulate DNA replication and coupled late gene expression is observed in a cell line derived from Spodoptera frugiperda but not in a cell line derived from Trichoplusia ni. This finding suggested that IE-2 may exert cell line-specific or hostspecific effects. To examine the role of ie-2 in the context of infection and its possible influence on the host range, we constructed recombinants of AcMNPV containing deletions of different functional regions within ie-2 and characterized them in cell lines and larvae of S. frugiperda and T. ni. The ie-2 mutant viruses exhibited delays in viral DNA synthesis, late gene expression, budded virus production, and occlusion body formation in SF-21 cells but not in TN-5B1-4 cells. In TN-5B1-4 cells, the ie-2 mutants produced more budded virus and fewer occlusion bodies but the infection proceeded without delay. Examination of the effects of ie-2 and the respective mutants on immediate-early viral promoters in transient expression assays revealed striking differences in the relative levels of expression and differences in responses to ie-2 and its mutant forms in different cell lines. In T. ni and S. frugiperda larvae, the infectivities of the occluded form of ie-2 mutant viruses by the normal oral route of infection was 100-and 1,000-fold lower, respectively, than that of wild-type AcMNPV. The reduction in oral infectivity was traced to the absence of virions within the occlusion bodies. The infectivity of the budded form of ie-2 mutants by hemocoelic injection was similar to that of wild-type virus in both species. Thus, ie-2 mutants are viable but exhibit cell line-specific effects on temporal regulation of the infection process. Due to its effect on virion occlusion, mutants of IE-2 were essentially noninfectious by the normal route of infection in both species tested. However, since budded viruses exhibited normal infectivity upon hemocoelic injection, we conclude that ie-2 does not affect host range per se. The possibility that IE-2 exerts tissue-specific effects has not been ruled out.

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Differential requirements for baculovirus late expression factor genes in two cell lines

Cited by 87 publications

References 65 publications

Baculoviruses: diversity, evolution and manipulation of insects

Baculoviruses: diversity, evolution and manipulation of insects

The Baculoviruses Occlusion‐Derived Virus: Virion Structure and Function

In Vivo and In Vitro Analysis of Baculovirus ie-2 Mutants

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