2019
DOI: 10.3389/fimmu.2019.00915
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Differential Responses of Bovine Monocyte-Derived Macrophages to Infection by Neospora caninum Isolates of High and Low Virulence

Abstract: Neospora caninum , a protozoan parasite closely related to Toxoplasma gondii , represents one of the main causes of abortion in cattle. Macrophages (MØs) are mediators of the innate immune response against infection and likely one of the first cells encountered by the parasite during the host infection process. In this study, we investigated in vitro how high or low virulent isolates of N. caninum (Nc-Spain7 and Nc-Spain1H, respect… Show more

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Cited by 31 publications
(60 citation statements)
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“…infection of human monocyte-derived dendritic cells resulted in reduced expression of CD1b [52]. Similar results were found in bovine monocyte-derived macrophages infected with Neospora caninum [53]. These results contrast with those found in the present study, in which the number of CD1b+ DC increased significantly in HLNs and livers at 18 dpi compared to that in the UC group.…”
Section: Discussionsupporting
confidence: 59%
See 1 more Smart Citation
“…infection of human monocyte-derived dendritic cells resulted in reduced expression of CD1b [52]. Similar results were found in bovine monocyte-derived macrophages infected with Neospora caninum [53]. These results contrast with those found in the present study, in which the number of CD1b+ DC increased significantly in HLNs and livers at 18 dpi compared to that in the UC group.…”
Section: Discussionsupporting
confidence: 59%
“…The difference in the expression of CD1b in the two parasites may be related to differences in the host response to N. caninum and F. hepatica infections in cattle and sheep, respectively. Thus, in N. caninum-infected cattle, elevated reactive oxygen species (ROS), IL-10 and IFN-γ production has been reported [53], whereas in F. hepatica-infected sheep, decreased ROS production [54] and decreased IFN-γ gene expression have been observed [15].…”
Section: Discussionmentioning
confidence: 99%
“…In mice, adoptively transferred parasitised DCs confer exacerbated dissemination with elevated parasite loads (Lambert et al, 2006;Fuks et al, 2012;Kanatani et al, 2017). Hypermigratory responses of mononuclear phagocytes (DCs, monocytes, macrophages, microglia) appear to be a conserved feature across host species (human, mouse, bovine) and are also induced by the related coccidian Neospora caninum (Collantes-Fernandez et al, 2012;Garcia-Sanchez et al, 2019). Yet, while hypermigration in DCs/monocytes/macrophages/microglia is induced by all T. gondii and N. caninum strains tested to date, measurable differences exist between parasite strains/lineages in the magnitude of induction of the hypermigratory phenotype, as defined in vitro.…”
Section: The Hypermigratory Phenotypementioning
confidence: 99%
“…Previously, we used in vitro and in vivo models to characterize two N. caninum isolates with marked differences in virulence: Nc-Spain7 and Nc-Spain1H, previously classified as high-and lowvirulence isolates, respectively [6][7][8]. Specifically, in bovine trophobast cells [9][10][11] and macrophages [12], Nc-Spain7 showed an increased infection and proliferation rates, whereas Nc-Spain1H displayed a reduced proliferation associated to a higher stimulation of immune responses. However, in vitro models cannot mimic the complex architecture of the bovine placenta, as they lack the microenvironmental influences and the host ability to compensate for stress conditions.…”
Section: Introductionmentioning
confidence: 99%