Differential responses of HSPs to heat stress in slow and fast regions of rat gastrocnemius muscle

Oishi, Yasuharu; Taniguchi, Kohachi; Matsumoto, Hisahiro; Ishihara, Akihiko; Ohira, Yoshinobu; Roy, Roland R.

doi:10.1002/mus.10476

Cited by 34 publications

(35 citation statements)

References 33 publications

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“…Twenty-four hours following SWD, the treatment leg was re-measured to ensure that the second biopsy was taken from the exact location that SWD was performed. Based oV of the literature, we hypothesized that Hsp expression would be at its peak 24 h following heat treatment (Oishi et al 2003a;Selsby and Dodd 2005).…”

Section: Methodsmentioning

confidence: 99%

“…Animal models have shown that Hsp induction in skeletal muscle, occurs at temperatures over 40°C (Oishi et al 2003a;Selsby and Dodd 2005). Skeletal muscle heat shock can provide protection from oxidative stress (Selsby and Dodd 2005;Selsby et al 2007;Smolka et al 2000), reduce muscle wasting during immobilization (Naito et al 2000;Selsby and Dodd 2005), enhance regrowth (Selsby et al 2007), proliferation (Uehara et al 2004), and attenuate the severity of muscle damage (Maglara et al 2003;McArdle et al 2004;Miyabara et al 2006).…”

Section: Introductionmentioning

confidence: 99%

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Diathermy treatment increases heat shock protein expression in female, but not male skeletal muscle

Touchberry

Richmond

et al. 2007

Eur J Appl Physiol

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Heat shock treatments have been used to appreciably increase heat shock protein 70 (Hsp70) content and the phosphorylation of heat shock protein 27 (Hsp27p). The purpose of this investigation was to determine whether diathermy can increase Hsp70 and Hsp27p content in skeletal muscle. Fourteen subjects (7 males and 7 females, 18-35 years) received a muscle biopsy from the v. lateralis and then underwent 20 min of diathermy followed by 20 min of hot pack heating on the contralateral leg. Twenty-four hours following treatment, a second muscle biopsy was performed on the treated leg. All samples were analyzed for Hsp70 and Hsp27p content using western immunoblotting. Images of the blots were obtained and analyzed via densitometry. A paired t-test was used to examine differences in heat shock protein content between the treated and untreated legs. Twenty-four hours following the heat treatment, female subjects significantly (P < 0.05) increased Hsp70 (+58%) and Hsp27p (+100%) content compared to the untreated leg. Male subjects had non-significant increases in Hsp70 (+35%) and Hsp27p (+32%) skeletal muscle content. These results implicate that diathermy can be an effective means to induce Hsp70 and Hsp27p in human skeletal muscle.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Diathermy treatment increases heat shock protein expression in female, but not male skeletal muscle

Touchberry

Richmond

et al. 2007

Eur J Appl Physiol

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“…Researchers generally have considered and reported that the up-regulation of HSP proteins in cells is induced when the body temperature is elevated by 38C to 58C 38 or above 408C in animal models. 39 However, Nussbaum and Locke 40 showed that the increases in HSP from repeated ultrasound treatments were not due to increased temperature but likely due to the mechanical properties introduced to the cell. As discussed, shortwave diathermy has been shown to increase HSP expression in human skeletal muscle.…”

Section: Hsp70 and Hsp27p:tmentioning

confidence: 99%

Intramuscular Heating Through Fluidotherapy and Heat Shock Protein Response

Vardiman

Jefferies

Touchberry

et al. 2013

Journal of Athletic Training

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Context: Therapeutic modalities that can increase intramuscular temperature commonly are used to treat injuries in the clinical setting. Researchers recently have suggested that the physiologic changes occurring during an increase in temperature also could provide a cytoprotective effect for exerciseinduced muscle damage.Objective(s): To determine if the Fluidotherapy treatment increases the inducible expression of heat shock protein (HSP), to identify the rate of heating that occurs in the lower extremity with Fluidotherapy treatment, and to evaluate the relationship between the inducible expression of HSP and temperature.Design: Controlled laboratory study. Setting: Laboratory.Patients or Other Participants: Six male (age ¼ 21.67 6 1.63 years, height ¼ 180.09 6 4.83 cm, mass ¼ 87.60 6 10.51 kg) and 6 female (age ¼ 24.60 6 4.59 years, height ¼ 151.05 6 35.76 cm, mass ¼ 55.59 6 14.58 kg) college-aged students.Intervention(s): One lower extremity was randomly selected to receive the heat treatment, and the other extremity received no treatment.Main Outcome Measure(s): We measured intramuscular temperature every 10 minutes, determining peak intramuscular temperature by 2 identical sequential measurements, and we analyzed the time to peak temperature. We analyzed the amount of HSP70 expression and HSP27P:T (ratio of HSP27 to the total HSP27 expression) in the gastrocnemius and soleus muscles and measured baseline skinfold thickness and estradiol levels.Results: Fluidotherapy increased intramuscular temperature by 5.66 6 0.788C (t 11 ¼ 25.67, P , .001) compared with baseline temperature, with a peak temperature of 39.088C 6 0.398C occurring at 84.17 6 6.69 minutes. We did not find a heat treatment effect for HSP70 or HSP27P:T in the gastrocnemius or soleus muscles (P . .05). Peak temperature and the percentage change of HSP70 were positively correlated for the gastrocnemius and soleus muscles (P , .05). We found no other correlations for skinfold thickness, sex, or estradiol levels (P . .05). No effect of sex for skinfold thickness or estradiol levels at baseline was discovered (P . .05).Conclusions: This Fluidotherapy protocol increased the intramuscular temperature to a therapeutic level; however, it did not stimulate inducible HSP70 or HSP27P:T in the soleus and gastrocnemius muscles regardless of sex or skinfold thickness. These data confirmed that Fluidotherapy is an effective heating modality but suggested it is not an effective method for stimulating an HSP response in the lower limb.Key Words: heat shock protein 27, heat shock protein 70, exercise-induced muscle damage Key PointsHeat shock protein concentrations did not increase with Fluidotherapy treatment in the lower extremity. Therapeutic levels of temperature within commonly accepted treatment times occurred with Fluidotherapy. Fluidotherapy effectively increased the temperature of extremity tissue.

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“…Furthermore, overexpression of HSP27 also prevented muscle fiber atrophy of the soleus muscle induced by disuse in rats (5). HSPs are expressed in response to several types of stress such as heat (14,15) and exercise (12). Exercise increased HSP72 expression (2) and attenuated skeletal muscle fiber atrophy (3) in streptozotocin Nonaka K et al 294 (STZ)-induced diabetic rats.…”

mentioning

confidence: 99%

Heat stress attenuates skeletal muscle atrophy of extensor digitorum longus in streptozotocin-induced diabetic rats

Nonaka¹,

Une²,

Akiyama³

2015

Acta Physiologica Hungarica

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To investigate whether heat stress attenuates skeletal muscle atrophy of the extensor digitorum longus (EDL) muscle in streptozotocin-induced diabetic rats, 12-week-old male Wistar rats were randomly assigned to four groups (n = 6 per group): control (Con), heat stress (HS), diabetes mellitus (DM), and diabetes mellitus/heat stress (DM + HS). Diabetes was induced by intraperitoneal injection of streptozotocin (50 mg/kg). Heat stress was induced in the HS and DM + HS groups by immersion of the lower half of the body in hot water at 42 °C for 30 min; it was initiated 7 days after injection of streptozotocin, and was performed once a day, five times a week for 3 weeks. The muscle fiber cross-sectional area of EDL muscles from diabetic and non-diabetic rats was determined; heat stress protein (HSP) 72 and HSP25 expression levels were also analyzed by western blotting. Diabetes-induced muscle fiber atrophy was attenuated upon heat stress treatment in diabetic rats. HSP72 and HSP25 expression was upregulated in the DM + HS group compared with the DM group. Our findings suggest that heat stress attenuates atrophy of the EDL muscle by upregulating HSP72 and HSP25 expression.Keywords: extensor digitorum longus muscle, heat stress, HSP72, HSP25, skeletal muscle atrophy, streptozotocin-induced diabetes Diabetes mellitus (DM) induces skeletal muscle atrophy (7, 19) and decreases muscle strength (7, 9). Consequently, patients with diabetes have a higher risk of falls because of reduced muscle strength (8, 16) and DM-induced-muscle atrophy. Where DM induces skeletal muscle atrophy, fast-twitch fibers are prone to greater atrophy than are slow-twitch fibers (1). Fast-twitch fibers are responsible for high force/power/speed production and maintaining physical balance during postural perturbation. Hence, atrophy of fast-twitch muscle increases the risk of falling in DM patients. Therefore, an efficient strategy is required to attenuate muscle atrophy in order to reduce the risk of falls in DM patients. Heat shock proteins (HSPs) have a protective effect on muscle atrophy. Senf et al. reported that HSP70 overexpression prevented rat soleus muscle fiber atrophy induced by hind limb immobilization (18). Furthermore, overexpression of HSP27 also prevented muscle fiber atrophy of the soleus muscle induced by disuse in rats (5). HSPs are expressed in response to several types of stress such as heat (14, 15) and exercise (12). Exercise increased HSP72 expression (2) and attenuated skeletal muscle fiber atrophy (3) in streptozotocin

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Differential responses of HSPs to heat stress in slow and fast regions of rat gastrocnemius muscle

Cited by 34 publications

References 33 publications

Diathermy treatment increases heat shock protein expression in female, but not male skeletal muscle

Diathermy treatment increases heat shock protein expression in female, but not male skeletal muscle

Intramuscular Heating Through Fluidotherapy and Heat Shock Protein Response

Heat stress attenuates skeletal muscle atrophy of extensor digitorum longus in streptozotocin-induced diabetic rats

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