Differential Role of Cation and Anion Exchange in Lymphocyte pH Regulation

Grinstein, Sergio; Garcia-Soto, J. J.; Mason, Mike J.

doi:10.1002/9780470513699.ch5

Cited by 8 publications

(2 citation statements)

References 14 publications

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“…Activation of this exchanger occurs by phosphorylation (Bianchini et al, ; Tominaga and Barber, ; Moor and Fliegel, ; Takahashi et al, ; Yan et al, ; Kintner et al, ; Luo et al, ; Malo et al, ; Meima et al, ), intracellular acidosis (Lagadic‐Gossmann et al, ; Casey et al, ), or osmotic stress (Holt et al, ). Additionally, NHE1 has been shown to become activated as a result of growth factor stimulation (Moolenaar et al, ; Grinstein et al, ; Strazzabosco et al, ) and integrin activation (Tominaga and Barber, ). Previously, we have shown that its function is also necessary for mouse mammary branching morphogenesis, and that its inhibition alters growth factor–induced signaling (Jenkins et al, ).…”

Section: Introductionmentioning

confidence: 99%

Na⁺/H⁺ exchanger 1 (NHE1) function is necessary for maintaining mammary tissue architecture

Jenkins

Debnath

Varriano

et al. 2013

Developmental Dynamics

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Background: The mammary gland is an ideal model to study the link between form and function in normal tissue. Perhaps as interesting as the cues necessary to generate this structure are the signals required to maintain its branched architecture over the lifetime of the organism, since likely these pathways are de-regulated in malignancies. Previously, we have shown that the Na 1 /H 1 exchanger 1 (NHE1), a critical regulator of intracellular pH, was necessary for mammary branching morphogenesis. Here we provide strong evidence that NHE1 function is also necessary for maintaining mammary branched architecture. Results: Inhibition of NHE1 with 5-N-Methy-N-isobutyl amiloride (MIA) on branched structures resulted in a rapid (within 24 hr) and reversible loss of branched architecture that was not accompanied by any overt changes in cell proliferation or cell death. NHE1 inhibition led to a significant acidification of intracellular pH in the branched end buds that preceded a number of events, including altered tissue polarity of myoepithelial cells, loss of NHE1 basal polarity, F-actin rearrangements, and decreased Ecadherin expression. Conclusions: Our results implicate NHE1 function and intracellular pH homeostasis as key factors that maintain mammary tissue architecture, thus, indirectly allowing for mammary function as a milk-providing (form) and -producing (function) gland. Developmental Dynamics 243:229-242, 2014. V C 2013 Wiley Periodicals, Inc.Key words: mammary tissue architecture; mammary epithelial ducts; sodium hydrogen exchanger; NHE1; intracellular pH; tissue polarity; cellular polarity Key Findings:NHE1 inhibition causes a rapid loss of mammary branched architecture. NHE1 function is necessary for mammary tissue organization and cellular polarity. NHE1 inhibition decreases E-cadherin expression.

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Section: Introductionmentioning

confidence: 99%

Na⁺/H⁺ exchanger 1 (NHE1) function is necessary for maintaining mammary tissue architecture

Jenkins

Debnath

Varriano

et al. 2013

Developmental Dynamics

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“…Some of the loop integrals will be divergent and, analogously to the pure light sector case [20,22,23,15], they have to be related to physical parameters. The resulting strong chiral Lagrangian has the following form [24,25,26,27,28,29,30]:…”

Section: Introductionmentioning

confidence: 99%

Isgur-Wise function within a modified heavy-light chiral quark model

Eeg

Kumerički

2010

Phys. Rev. D

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We consider the Isgur-Wise function ξ(ω) within a new modified version of a heavy-light chiral quark model. While early versions of such models gave too small absolute value of the slope, namely ξ ′ (1) ≃ −0.4 to −0.3, we show how extended version(s) may lead to values around −1, in better agreement with recent measurements. This is obtained by introducing a new mass parameter in the heavy quark propagator.We also shortly comment on the consequences for the decay modes B → DD.

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pHi regulation in Ehrlich mouse ascites tumor cells: Role of sodium-dependent and sodium-independent chloride-bicarbonate exchange

Kramhøft¹,

Hoffmann²,

Simonsen³

1994

J. Membarin Biol.

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pHi recovery in acid-loaded Ehrlich ascites tumor cells and pHi maintenance at steady-state were studied using the fluorescent probe BCECF. Both in nominally HCO3(-)-free media and at 25 mM HCO3-, the measured pHi (7.26 and 7.82, respectively) was significantly more alkaline than the pHi value calculated assuming the transmembrane HCO3- gradient to be equal to the Cl- gradient. Thus, pHi in these cells is not determined by the Cl- gradient and by Cl-/HCO3- exchange. pHi recovery following acid loading by propionate exposure, NH4+ withdrawal, or CO2 exposure is mediated by amiloride-sensitive Na+/H+ exchange in HCO3(-)-free media, and in the presence of HCO3- (25 mM) by DIDS-sensitive, Na+)-dependent Cl-/HCO3- exchange. A significant residual pHi recovery in the presence of both amiloride and DIDS suggests an additional role for a primary active H+ pump in pHi regulation. pHi maintenance at steady-state involves both Na+/H+ exchange and Na(+)-dependent Cl-/HCO3- exchange. Acute removal of external Cl- induces a DIDS-sensitive, Na(+)-dependent alkalinization, taken to represent HCO3- influx in exchange for cellular Cl-. Measurements of 36Cl- efflux into Cl(-)-free gluconate media with and without Na+ and/or HCO3- (10 mM) directly demonstrate a DIDS-sensitive, Na(+)-dependent Cl-/HCO3- exchange operating at slightly acidic pHi (pHo 6.8), and a DIDS-sensitive, Na+)-independent Cl-/HCO3- exchange operating at alkaline pHi (pHo 8.2).

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Differential Role of Cation and Anion Exchange in Lymphocyte pH Regulation

Cited by 8 publications

References 14 publications

Na⁺/H⁺ exchanger 1 (NHE1) function is necessary for maintaining mammary tissue architecture

Na⁺/H⁺ exchanger 1 (NHE1) function is necessary for maintaining mammary tissue architecture

Isgur-Wise function within a modified heavy-light chiral quark model

pHi regulation in Ehrlich mouse ascites tumor cells: Role of sodium-dependent and sodium-independent chloride-bicarbonate exchange

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Differential Role of Cation and Anion Exchange in Lymphocyte pH Regulation

Cited by 8 publications

References 14 publications

Na+/H+ exchanger 1 (NHE1) function is necessary for maintaining mammary tissue architecture

Na+/H+ exchanger 1 (NHE1) function is necessary for maintaining mammary tissue architecture

Isgur-Wise function within a modified heavy-light chiral quark model

pHi regulation in Ehrlich mouse ascites tumor cells: Role of sodium-dependent and sodium-independent chloride-bicarbonate exchange

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Na⁺/H⁺ exchanger 1 (NHE1) function is necessary for maintaining mammary tissue architecture

Na⁺/H⁺ exchanger 1 (NHE1) function is necessary for maintaining mammary tissue architecture