Differential sensitivity of d-galactose-binding lectins from fruits of dwarf elder (Sambucus ebulus L.) to a simulated gastric fluid

Jiménez, Pilar; Tejero, Jesús; Cabrero, Patricia; Córdoba-Díaz, Damián; Girbés, Tomás

doi:10.1016/j.foodchem.2012.09.011

Cited by 22 publications

(42 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As shown in Figure 2, both proteins, which we named ebulin blo (the smallest) and SELblo, moved as single and well-defined protein peaks in the absence of 2-ME, with apparent Mr close to 60,000 and 68,000, respectively. Both proteins were very close to those found in the extracts of green fruits [19]. In the presence of 2-ME, both proteins dissociated to yield two subunits of different apparent Mr, in the case of ebulin blo, presumably an A-type with an apparent Mr of 30,000, a B-type chain with an apparent Mr of 34,000 and, in the case of SELblo, two apparently identical B-type chains of 38,000.…”

Section: Resultssupporting

confidence: 67%

“…The major peak accounted for SELblo and the other one for ebulin blo. The ratio of SELblo to ebulin blo in blossoms was higher than that reported for ebulin f in early and late green fruits [19]. …”

Section: Resultsmentioning

confidence: 56%

“…The isolation of these lectins was performed as reported recently to avoid the water-soluble mucilage that binds to the chromatography resin [19]. The procedure involves the first step of affinity chromatography on acid-treated Sepharose 6B, and the bound protein was eluted with lactose.…”

Section: Resultsmentioning

confidence: 99%

“…Ebulin toxins are known as type 2 (two chains) ribosome-inactivating proteins, or RIPs, since they have two chains, an A chain with N -glycosidase activity (E.C.3.2.2.22) on 28S rRNA, which is responsible for the irreversible inactivation of ribosomes, and a B chain with d -galactose-specific lectin activity [5,16,17]. S. ebulus also contains lectins of the B-B type with d -galactose-specific activity only, like SELld in leaves [17], SELlm in shoots [18] and SELfd in fruits [19,20]. …”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Isolation and Molecular Characterization of Two Lectins from Dwarf Elder (Sambucus ebulus L.) Blossoms Related to the Sam n1 Allergen

Jiménez

Cabrero

Basterrechea

et al. 2013

Toxins

Self Cite

View full text Add to dashboard Cite

Sambucus species contain a number of lectins with and without antiribosomal activity. Here, we show that dwarf elder (Sambucus ebulus L.) blossoms express two d-galactose-binding lectins that were isolated and purified by affinity chromatography and gel filtration. These proteins, which we named ebulin blo (A-B toxin) and SELblo (B-B lectin)—blo from blossoms—were subjected to molecular characterization and analysis by MALDI-TOF mass spectrometry and tryptic peptide fingerprinting. Both lectins share a high degree of amino acid sequence homology with Sambucus lectins related to the Sam n1 allergen. Ebulin blo, but not SELblo, was highly toxic by nasal instillation to mice. Overall, our results suggested that both lectins would belong to an allergen family exemplified by Sam n1 and could trigger allergy responses. Furthermore, they raise a concern about ebulin blo toxicity.

show abstract

Section: Resultssupporting

confidence: 67%

Section: Resultsmentioning

confidence: 56%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Isolation and Molecular Characterization of Two Lectins from Dwarf Elder (Sambucus ebulus L.) Blossoms Related to the Sam n1 Allergen

Jiménez

Cabrero

Basterrechea

et al. 2013

Toxins

Self Cite

View full text Add to dashboard Cite

show abstract

“…Lectins are widely distributed in nature, being found in animals, plants, and microorganisms [5][6][7]. In plants, the main source of lectins is the seeds, but they are also found in the fruits, stems, and leaves [8,6,9].…”

Section: Introductionmentioning

confidence: 99%

Antibacterial and Hemolytic Activity of a new Lectin Purified from the Seeds of Sterculia Foetida L.

Braga

Lacerda

Medeiros

et al. 2014

Appl Biochem Biotechnol

View full text Add to dashboard Cite

The aim of this study was to isolate, characterize, and verify possible antibacterial and hemolytic activity for a lectin found in the seeds of Sterculia foetida L. Purification of the lectin from S. foetida (SFL) was realized with ion exchange chromatography DEAE-Sephacel coupled to HPLC. The purity and the molecular weight was determined by SDS-PAGE. The isolated SFL was characterized as to its glycoprotein nature, and sugar specificity, as well as resistance to pH, temperature, denaturing agents, reduction, oxidation, and chelation. A microdilution method was used to determine antibacterial activity, and hemolytic activity was observed in human erythrocytes. The SFL has a molecular weight of 17 kDa, and a carbohydrate content of 53 μg/mL, specific for arabinose and xylose, and is resistant to treatment with urea, sensitive to treatment with sodium metaperiodate and β-mercaptoethanol, and in the presence of EDTA lost its hemagglutinating activity (HA). However, in the presence of divalent cations (Ca(2 +) and Mn(2 +)) the HA was increased. The SFL remained active even after incubation at 80 °C, and, within pH values of between 5 and 11. The SFL inhibited the bacterial growth of all the tested strains and caused little hemolysis in human erythrocytes when compared to the positive control Triton X-100.

show abstract

Effects of Short-term Heating on Total Polyphenols, Anthocyanins, Antioxidant Activity and Lectins of Different Parts of Dwarf Elder (Sambucus ebulus L.)

Jiménez

Cabrero

Basterrechea

et al. 2014

Plant Foods Hum Nutr

View full text Add to dashboard Cite

Dwarf elder (Sambucus ebulus L.) berries are rich in health-promoting phytochemicals such as polyphenols and anthocyanins, and display a significant antioxidant activity. They are also rich in two lectins (ebulin f and SELfd) that share amino acid sequence homology with the elderberry allergen Sam n1 present in Sambucus nigra pollen and fruits. Ebulin f displays toxicity by oral ingestion. This study was aimed at eliminating the toxicity of these lectins whilst having little or no effect on the antioxidant properties of dwarf elder berries. We thus investigated the potential effects of incubation in a boiling water bath of extracts from several parts of the plant on total polyphenol content, antioxidant activity, total anthocyanins, cyanidin-3-glycoside content, and the sensitivity of purified dwarf elder fruit lectins to a simulated gastric fluid. The study shows that five minutes of said heat treatment fully sensitized both lectins to pepsin digestion, whilst minimally reducing phenol and antioxidant as well as free radical scavenging activities to below 13%. It proved possible to eliminate the potential risks derived from the presence of lectins in dwarf elder juices without any significant reduction in the content of the antioxidant compounds. Dwarf elder berries may thus be a valuable nutritional source.

show abstract

Differential sensitivity of d-galactose-binding lectins from fruits of dwarf elder (Sambucus ebulus L.) to a simulated gastric fluid

Cited by 22 publications

References 30 publications

Isolation and Molecular Characterization of Two Lectins from Dwarf Elder (Sambucus ebulus L.) Blossoms Related to the Sam n1 Allergen

Isolation and Molecular Characterization of Two Lectins from Dwarf Elder (Sambucus ebulus L.) Blossoms Related to the Sam n1 Allergen

Antibacterial and Hemolytic Activity of a new Lectin Purified from the Seeds of Sterculia Foetida L.

Effects of Short-term Heating on Total Polyphenols, Anthocyanins, Antioxidant Activity and Lectins of Different Parts of Dwarf Elder (Sambucus ebulus L.)

Contact Info

Product

Resources

About