Differential sensitivity of Madin-Darby canine kidney (MDCK) cells to epinephrine

Abstract

Catecholamines regulate a variety of cellular functions in the mammalian kidney. The present study was aimed to investigate the differential sensitivity of Madin-Darby Kidney Cells (MDCK cells) to epinephrine in a dose-dependent manner. The loss of adhesion and altered cell shape were observed in MDCK cells. The presence of apoptosis and necrosis were studied by the fluorescence microscope and Confocal Laser Scanning Microscope (CLSM). Scanning Electron Microscope (SEM) analysis showed several surface microvilli, and cells were rounded having ruffled and crenated surface. Agarose gel electrophoresis study showed the presence of smearing, which further confirms the occurrence of necrosis. The fluorescence staining study showed the increased reactive oxygen species (ROS) level. Up-regulation of p53, bax, and caspase 3 mRNA expressions was evidenced by quantitative PCR (qPCR). Caspase 3 activity was also increased in epinephrine treated cells. Our experimental results do not imply that the epinephrine should not be used in the clinical treatments. However, our results add a research note of caution on the possible cytotoxic effect of maximal doses of epinephrine over a prolonged time.

Nitroso‐Oxidative Stress, Acute Phase Response, and Cytogenetic Damage in Wistar Rats Treated with Adrenaline

“…In our work, tested catecholamine (1.5 and 3 mg/kg) decreased the PCE/NCE ratio indicating its cytotoxicity in bone marrow. Muthuraman et al [70] showed that adrenaline possessed a cytotoxic effect and affects DNA fragmentation in a dose-dependent manner in MDCK cells.…”

Renal‐protective and ameliorating impacts of omega‐3 fatty acids against aspartame damaged MDCK cells

“…MDCK cells were cultured and grown in 96‐well plates, and allowed to adhere for 24 h at 37°C. Cells were treated with different concentration of aspartame (2, 4, 6, 8, & 10 mM) and omega‐3 fatty acids (250 µM, 500 µM, 750 µM, 1 mM, & 1.25 mM) individual and combined form for 48 h. At the end of treatment, the cell viability was determined .…”

“…After 90% cell confluence, the cells were treated with 10 mM of and 1.25 mM of omega‐3 fatty acids. The morphology of MDCK cells was visualized with the Scanning electron microscope (SEM) . Cells were fixed in a fixative containing 2.5% glutaraldehyde/0.1 M PBS for 30 min at 4°C and postfixed with 1% osmium tetroxide for 30 min at room temperature after repeated wash with PBS.…”