Differential solubilization of proteins, phospholipids, and cholesterol of erythrocyte membranes by detergents

Kirkpatrick, Francis H.; Gordesky, Stanley E.; Marinetti, G.V.

doi:10.1016/0005-2736(74)90254-5

Cited by 108 publications

(30 citation statements)

References 33 publications

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“…The actual cause of the particle aggregation must be related to another effect of the Triton X-100, possibly a lateral separation of lipid components as a result of preferential interactions with the detergent. Differences in the interaction between Triton X-100 and the various lipids are suggested by the differential solubilization of lipids from the membrane [42]. In a model system such differences have been demonstrated for phosphatidylcholine and sphingomyelin [43].…”

Section: Discussionmentioning

confidence: 97%

“…From observations on recombinant vesicles Yu and Branton [10] concluded that spectrin-actin was bound to integral proteins, and that this association caused the particles to aggregate at low pH. However, the recombinant vesicles were prepared from a crude Triton X-100 extract which contains a considerable amount of phospholipids, including phosphatidylserine [32,42]. This could also be responsible for the binding of spectrin-actin.…”

Section: Discussionmentioning

confidence: 99%

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The lateral distribution of intramembrane particles in the erythrocyte membrane and recombinant vesicles

Gerritsen

Verkleij

Deenen

1979

Biochimica et Biophysica Acta (BBA) - Biomembranes

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SummaryTriton X-100 (in concentrations which did not cause a significant solubilization of membrane material) caused aggregation of the intramembrane particles of human erythrocyte ghosts.Ghosts from which the extrinsic proteins had been removed by alkali treatment showed a temperature-induced aggregation of the particles. With virtually no spectrin present, the particles in these stripped ghosts could still be aggregated by manipulations with ionic strength and pH, or by the addition of calcium.Recombinant vesicles were made from a Triton X-100 extract and a mixture of phospholipids with a composition which resembled that of the inner monolayer of erythrocyte membrane. In these recombinants the same manipulations with ionic strength and pH and the addition of calcium caused a rearrangement of the particles, resulting in the appearance of particle-free areas. In recombinants prepared from a Triton X-100 extract and egg phosphatidylcholine the lateral distribution of the particles was not altered by these manipulations.It is concluded that in the erythrocyte membrane the intramembrane particles can be aggregated by effects of external agents on lipid components. In this light the role of spectrin in stabilizing the membrane by interactions with lipids in the inner monolayer is discussed.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

The lateral distribution of intramembrane particles in the erythrocyte membrane and recombinant vesicles

Gerritsen

Verkleij

Deenen

1979

Biochimica et Biophysica Acta (BBA) - Biomembranes

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“…2,23,24 For example, sodium lauryl sulfate is capable of lysing biological membranes by lipid solubilization and protein denaturation and solubilization. 25,26 Other enhancers remove extracellular Ca 2þ leading to disruption of actin filaments, disruption of adherent junctions, and diminished cell adhesion. 27 For example, palmitoyl carnitine has displayed clear signs of cell damage at effective concentrations (LDH leakage and inability of the cells to recover full viability).…”

Section: Discussionmentioning

confidence: 99%

The effect of ΔG on the transport and oral absorption of macromolecules

Salama¹,

Fasano²,

Thakar³

et al. 2004

Journal of Pharmaceutical Sciences

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“…The present studies on the effects of sodium deoxycholate were performed because detergents are now widely used in the study of biomembranes, both to isolate components and to probe the structure of the membrane itself (Kirkpatrick et al, 1974). The conversion of cytochrome P450 into cytochrome P420 in the presence of sodium deoxycholate (Fig.…”

Section: Discussionmentioning

confidence: 99%

Mechanism of inhibition by carbonyl cyanide m-chlorophenylhydrazone and sodium deoxycholate of cytochrome P-450-catalysed hepatic microsomal drug metabolism

Громова

Lyakhovich

1976

Biochemical Journal

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1. Treatment of liver microsomal fraction with 0.03-0.12% sodium deoxycholate and 0.005-0.06mM carbonyl cyanide m-chlorophenylhydrazone decreases phospholipiddependent hydrophobicity of the microsomal membrane, assayed by the kinetics of 8-anilinonaphthalene-l-sulphonate binding and ethyl isocyanide difference spectra. 2. Sodium deoxycholate at a concentration of 0.01 % lacks its detergent properties, but competitively inhibits aminopyrine binding and activates the initial rate of NADPH-cytochrome P450 reductase. In the presence of 0.03-0.09% sodium deoxycholate the ratelimiting factor in p-hydroxylation of aniline is the content of cytochrome P450, and that for N-demethylation of aminopyrine is the activity of NADPH-cytochrome P450 reductase. 3. Carbonyl cyanide m-chlorophenylhydrazone has no effect on the binding and metabolism of aniline; investigation of its inhibiting effect on aminopyrine N-demethylase established that the rate-limiting reaction is the dissociation of the enzyme-substrate complex in the microsomal preparations. 4. In the mechanism of action of carbonyl cyanide m-chlorophenylhydrazone the key step may be the electrostatic interaction of its protonated form and one of the forms of activated oxygen at the catalytic centre of cytochrome P450. 5. At least two different phospholipid-dependent hydrophobic zones are assumed to exist in the microsomal membrane, both coupled with cytochrome PA450. One of them reveals selective sensitivity to the protonation action of carbonyl cyanide mchlorophenylhydrazone and contains the 'binding protein' for type I substrates and NADPH-cytochrome P450 reductase; the other contains the cytochrome P450 haem group and binding sites for type II substrates.

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Differential solubilization of proteins, phospholipids, and cholesterol of erythrocyte membranes by detergents

Cited by 108 publications

References 33 publications

The lateral distribution of intramembrane particles in the erythrocyte membrane and recombinant vesicles

The lateral distribution of intramembrane particles in the erythrocyte membrane and recombinant vesicles

The effect of ΔG on the transport and oral absorption of macromolecules

Mechanism of inhibition by carbonyl cyanide m-chlorophenylhydrazone and sodium deoxycholate of cytochrome P-450-catalysed hepatic microsomal drug metabolism

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